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[NMR paper] Metal interactions of ?-synuclein probed by NMR amide-proton exchange
May 22, 2023 - 3:10 AM - by nmrlearner
nmrlearner's Avatar Metal interactions of ?-synuclein probed by NMR amide-proton exchange

The aberrant aggregation of ?-synuclein (?S), a disordered protein primarily expressed in neuronal cells, is strongly associated with the underlying mechanisms of Parkinson's disease. It is now established that ?S has a weak affinity for metal ions and that these interactions alter its conformational properties by generally promoting self-assembly into amyloids. Here, we characterised the nature of the conformational changes associated with metal binding by ?S using nuclear magnetic resonance...

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[NMR paper] Quantitative NMR analysis of the mechanism and kinetics of chaperone Hsp104 action on amyloid-?42 aggregation and fibril formation
May 22, 2023 - 3:10 AM - by nmrlearner
nmrlearner's Avatar Quantitative NMR analysis of the mechanism and kinetics of chaperone Hsp104 action on amyloid-?42 aggregation and fibril formation

The chaperone Hsp104, a member of the Hsp100/Clp family of translocases, prevents fibril formation of a variety of amyloidogenic peptides in a paradoxically substoichiometric manner. To understand the mechanism whereby Hsp104 inhibits fibril formation, we probed the interaction of Hsp104 with the Alzheimer's amyloid-?42 (A?42) peptide using a variety of biophysical techniques. Hsp104 is highly effective at suppressing the formation of Thioflavin T (ThT) reactive mature fibrils that are readily...

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[NMR paper] Expression and purification of 15N-labeled Fra a 1, a strawberry allergen, to prepare samples for NMR measurements
May 17, 2023 - 5:43 AM - by nmrlearner
nmrlearner's Avatar Expression and purification of 15N-labeled Fra a 1, a strawberry allergen, to prepare samples for NMR measurements

Raw strawberries contain allergens that cause oral allergic syndrome. Fra a 1 is one of the major allergens in strawberries and might decrease their allergenicity by heating, likely due to structural changes in the allergen leading to decreased recognition of the allergens in the oral cavity. In the present study, to understand the relationship between allergen structure and allergenicity, the expression and purification of 15N-labeled Fra a 1 were examined and the sample was used for NMR...

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[NMR paper] Using NMR Titration Experiments to Study E. coli FAS-II- and AcpP-Mediated Protein-Protein Interactions
May 15, 2023 - 9:27 PM - by nmrlearner
nmrlearner's Avatar Using NMR Titration Experiments to Study E. coli FAS-II- and AcpP-Mediated Protein-Protein Interactions

Acyl carrier proteins (ACPs) are central to many primary and secondary metabolic pathways. In E. coli fatty acid biosynthesis (FAB), the central ACP, AcpP, transports intermediates to a suite of partner proteins (PP) for iterative modification and elongation. The regulatory protein-protein interactions that occur between AcpP and the PP in FAB are poorly understood due to the dynamic and transient nature of these interactions. Solution-state NMR spectroscopy can reveal information at the atomic...

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