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Solid-State NMR Approaches to Internal Dynamics of Proteins: From Picoseconds to Microseconds and Seconds - ACS Publications
Apr 02, 2025 - 10:22 PM - by nmrlearner
nmrlearner's Avatar Solid-State NMR Approaches to Internal Dynamics of Proteins: From Picoseconds to Microseconds and Seconds - ACS Publications

Solid-State NMR Approaches to Internal Dynamics of Proteins: From Picoseconds to Microseconds and Seconds ACS Publications Read here
0 Replies | 4 Views
[NMR paper] Complete assignment of (1)H and (13)C NMR signals of monoglucosylated high-mannose type glycan attached to asparagine
Apr 02, 2025 - 8:39 AM - by nmrlearner
nmrlearner's Avatar Complete assignment of (1)H and (13)C NMR signals of monoglucosylated high-mannose type glycan attached to asparagine

Glc(1)Man(9)GlcNAc(2) (G1M9) glycan and other high mannose-type glycans play key roles in the quality control mechanisms of glycoprotein synthesis. The lectin-like proteins calnexin (CNX) and calreticulin (CRT) specifically recognize G1M9 glycan and assist newly synthesized glycoproteins to achieving correct folding. Nuclear magnetic resonance (NMR) spectroscopy is a unique method for analyzing the conformation, dynamics and interactions of glycans like G1M9 glycan and CNX/CRT. Accurate...

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0 Replies | 56 Views
[NMR paper] Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases
Apr 02, 2025 - 8:39 AM - by nmrlearner
nmrlearner's Avatar Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases

Substituting a single hydrogen atom in a protein by fluorine provides a probe for site-specific sensing by ^(19)F nuclear magnetic resonance (NMR) spectroscopy with minimal impact on the properties of the protein. Genetic encoding systems are presented for five different fluorinated analogues of phenylalanine: 2-, 3-, 4-fluorophenylalanine, 2,6-difluorophenylalanine, and 3,5-difluorophenylalanine. The systems allow the installation of each of these amino acids with high fidelity during in vivo...

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0 Replies | 15 Views
NMR Structure of Retinal Guanylate Cyclase Activating Protein 5 (GCAP5) with R22A Mutation That Abolishes Dimerization and Enhances Cyclase Activation - ACS Publications
Apr 01, 2025 - 8:23 PM - by nmrlearner
nmrlearner's Avatar NMR Structure of Retinal Guanylate Cyclase Activating Protein 5 (GCAP5) with R22A Mutation That Abolishes Dimerization and Enhances Cyclase Activation - ACS Publications

NMR Structure of Retinal Guanylate Cyclase Activating Protein 5 (GCAP5) with R22A Mutation That Abolishes Dimerization and Enhances Cyclase Activation ACS Publications Read here
0 Replies | 13 Views
Measuring Protein–Ligand Binding by Hyperpolarized Ultrafast NMR - ACS Publications
Apr 01, 2025 - 8:23 PM - by nmrlearner
nmrlearner's Avatar Measuring Protein–Ligand Binding by Hyperpolarized Ultrafast NMR - ACS Publications

Measuring Protein–Ligand Binding by Hyperpolarized Ultrafast NMR ACS Publications Read here
0 Replies | 13 Views
[NMR paper] Detection of liver mitochondrial oxaloacetate by NMR spectroscopy and membrane potential-dependent accumulation
Apr 01, 2025 - 8:23 PM - by nmrlearner
nmrlearner's Avatar Detection of liver mitochondrial oxaloacetate by NMR spectroscopy and membrane potential-dependent accumulation

Oxaloacetate (OAA) is a central liver metabolite fundamental to critical metabolic pathways. However, understanding OAA metabolism in the liver has been limited because the compound is very difficult to measure by mass spectroscopy and not abundant enough for detection by other methods. Here we describe a novel approach to quantifying OAA in liver mitochondria. Moreover, we provide evidence for membrane potential-dependent OAA accumulation in mitochondria during complex II-energized respiration...

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0 Replies | 51 Views
[NMR paper] Solid-State NMR Analysis of Schizosaccharomyces pombe Reveals Role of alpha -Amylase Family Enzymes in Cell Wall Structure and Function
Apr 01, 2025 - 8:12 AM - by nmrlearner
nmrlearner's Avatar Solid-State NMR Analysis of Schizosaccharomyces pombe Reveals Role of alpha -Amylase Family Enzymes in Cell Wall Structure and Function

The fission yeast Schizosaccharomyces pombe is a widely employed model organism for studying the eukaryotic cell cycle. Like plants and bacteria, S. pombe must build a cell wall in concert with its cell cycle, but how cell wall-synthesizing and remodeling enzymes mediate this process remains unclear. Here we characterize the functions of Aah1 and Aah3, two related S. pombe ?-amylases that are putative members of this evolutionarily conserved family of cell wall-modifying proteins. We found that...

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0 Replies | 26 Views
[NMR paper] Proton-Detected Solid-State NMR for Deciphering Structural Polymorphism and Dynamic Heterogeneity of Cellular Carbohydrates in Pathogenic Fungi
Apr 01, 2025 - 8:12 AM - by nmrlearner
nmrlearner's Avatar Proton-Detected Solid-State NMR for Deciphering Structural Polymorphism and Dynamic Heterogeneity of Cellular Carbohydrates in Pathogenic Fungi

Carbohydrate polymers in their cellular context display highly polymorphic structures and dynamics essential to their diverse functions, yet they are challenging to analyze biochemically. Proton-detection solid-state NMR spectroscopy offers high isotopic abundance and sensitivity, enabling rapid and high-resolution structural characterization of biomolecules. Here, an array of 2D/3D ¹ H-detection solid-state NMR techniques are tailored to investigate polysaccharides in fully protonated or...

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0 Replies | 24 Views
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