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[NMR paper] A 3D MAS NMR experiment utilizing through-space 15N-15N correlations.
Apr 28, 2017 - 2:27 PM - by nmrlearner
nmrlearner's Avatar A 3D MAS NMR experiment utilizing through-space 15N-15N correlations.

Related Articles A 3D MAS NMR experiment utilizing through-space 15N-15N correlations.

J Am Chem Soc. 2017 Apr 27;:

Authors: Donovan KJ, Silvers R, Linse S, Griffin RG

Abstract
We demonstrate a novel 3D NNC magic angle spinning (MAS) NMR experiment that generates 15N- 15N internuclear contacts in protein systems using an optimized 15N- 15N proton assisted recoupling (PAR) mixing period and a 13C dimension for improved resolution. The optimized PAR condition permits the acquisition of high signal-to-noise 3D data that enables backbone chemical shift assignments using a strategy that is complementary to current schemes. The spectra can also provide distance constraints. The utility of the experiment is demonstrated on an M0Ab1-42 fibril sample that yields high-quality data that is readily assigned and interpreted. The 3D NNC experiment therefore provides a powerful platform for solid-state protein studies and is broadly applicable to a variety of systems and experimental conditions.


PMID: 28447786 [PubMed - as supplied by publisher]



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0 Replies | 4 Views
Delivering strong 1H nuclear hyperpolarization levels and long magnetic lifetimes through signal amplification by reversible exchange
Apr 28, 2017 - 2:27 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Delivering strong 1H nuclear hyperpolarization levels and long magnetic lifetimes through signal amplification by reversible exchange

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Rayner, P.J., et al., Delivering strong 1H nuclear hyperpolarization levels and long magnetic lifetimes through signal amplification by reversible exchange. Proc Natl Acad Sci U S A, 2017. 114(16): p. E3188-E3194.


https://www.ncbi.nlm.nih.gov/pubmed/28377523


Hyperpolarization turns typically weak NMR and MRI responses into strong signals so that ordinarily impractical measurements become possible. The potential to revolutionize analytical NMR and clinical diagnosis through this approach reflect this area's most compelling outcomes. Methods to optimize the low-cost parahydrogen-based approach signal amplification by reversible exchange with studies on a series of biologically relevant nicotinamides and methyl nicotinates are detailed. These procedures involve specific 2H labeling in both the agent and catalyst and achieve polarization lifetimes of ca 2 min with 50% polarization in the case of methyl-4,6-d2 -nicotinate. Because a 1.5-T hospital scanner has an effective 1H polarization level of just 0.0005% this strategy should result in compressed detection times for chemically discerning measurements that probe disease. To demonstrate this technique's generality, we exemplify further studies on a range of pyridazine, pyrimidine, pyrazine, and isonicotinamide analogs that feature as building blocks in biochemistry and many... [Read More]
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Protein Crystallization Market Set to Witness an Uptick During 2020 : Persistence Market Research - MilTech
Apr 27, 2017 - 5:44 PM - by nmrlearner
nmrlearner's Avatar Protein Crystallization Market Set to Witness an Uptick During 2020 : Persistence Market Research - MilTech



Protein Crystallization Market Set to Witness an Uptick During 2020 : Persistence Market Research
MilTech
Various techniques used during protein crystallization technology are ion-exchange chromatography, high-performance liquid chromatography (HPLC), gel-electrophoresis, x-ray crystallography and nuclear magnetic resonance (NMR). On the basis of ...

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[NMR paper] Synthesis and hyperpolarisation of eNOS substrates for quantification of NO production by (1)H NMR spectroscopy.
Apr 27, 2017 - 5:44 PM - by nmrlearner
nmrlearner's Avatar Synthesis and hyperpolarisation of eNOS substrates for quantification of NO production by (1)H NMR spectroscopy.

Related Articles Synthesis and hyperpolarisation of eNOS substrates for quantification of NO production by (1)H NMR spectroscopy.

Bioorg Med Chem. 2017 May 15;25(10):2730-2742

Authors: Fernandez Diaz-Rullo F, Zamberlan F, Mewis RE, Fekete M, Broche L, Cheyne LA, Dall'Angelo S, Duckett SB, Dawson D, Zanda M

Abstract
Hyperpolarization enhances the intensity of the NMR signals of a molecule, whose in vivo metabolic fate can be monitored by MRI with higher sensitivity. SABRE is a hyperpolarization technique that could potentially be used to image nitric oxide (NO) production in vivo. This would be very important, because NO dysregulation is involved in several pathologies, including cardiovascular ones. The... [Read More]
0 Replies | 6 Views
[NMR paper] Water Proton NMR-A Tool for Protein Aggregation Characterization.
Apr 26, 2017 - 2:16 PM - by nmrlearner
nmrlearner's Avatar Water Proton NMR-A Tool for Protein Aggregation Characterization.

Related Articles Water Proton NMR-A Tool for Protein Aggregation Characterization.

Anal Chem. 2017 Apr 25;:

Authors: Taraban MB, DePaz RA, Lobo B, Yu YB

Abstract
Formulation stability is a critical attribute of any protein-based biopharmaceutical drug due to a protein's inherent tendency to aggregate. Advanced analytical techniques currently used for characterization of protein aggregates are prone to a number of limitations, and usually require additional manipulations with the sample, such as dilution, separation, labeling and use of special cuvettes. In the present work, we compared conventional techniques for the analysis of protein aggregates with a novel approach that employs the water proton transverse relaxation rate R2(1H2O). We explored differences in the sensitivity of conventional techniques-size-exclusion chromatography (SEC), micro-flow imaging (MFI), and dynamic light scattering (DLS)-and water NMR (wNMR) towards the presence of monoclonal antibody aggregates generated by different stresses. We demonstrate that wNMR outperformed SEC, DLS, and MFI in that it was most consistently sensitive to increases in both soluble and insoluble aggregates, including subvisible particles. The simplicity of wNMR, its sensitivity, and possibility of noninvasive measurements are unique advantages that would permit its application for more efficient and higher throughput optimization of protein... [Read More]
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