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(PDF) Spinning faster: protein NMR at MAS frequencies up to 126 kHz - ResearchGate
Mar 30, 2025 - 8:00 PM - by nmrlearner
nmrlearner's Avatar (PDF) Spinning faster: protein NMR at MAS frequencies up to 126 kHz - ResearchGate

(PDF) Spinning faster: protein NMR at MAS frequencies up to 126 kHz ResearchGate Read here
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Selective Methods Promote Protein Solid-State NMR - ACS Publications
Mar 30, 2025 - 8:00 PM - by nmrlearner
nmrlearner's Avatar Selective Methods Promote Protein Solid-State NMR - ACS Publications

Selective Methods Promote Protein Solid-State NMR ACS Publications Read here
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Automated Assignment of 15N And 13C Enrichment Levels in Doubly-Labeled Proteins - ACS Publications
Mar 30, 2025 - 8:00 PM - by nmrlearner
nmrlearner's Avatar Automated Assignment of 15N And 13C Enrichment Levels in Doubly-Labeled Proteins - ACS Publications

Automated Assignment of 15N And 13C Enrichment Levels in Doubly-Labeled Proteins ACS Publications Read here
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Protein Allostery Study in Cells Using NMR Spectroscopy - ACS Publications
Mar 30, 2025 - 8:00 PM - by nmrlearner
nmrlearner's Avatar Protein Allostery Study in Cells Using NMR Spectroscopy - ACS Publications

Protein Allostery Study in Cells Using NMR Spectroscopy ACS Publications Read here
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Lipid Nanodiscs for High-Resolution NMR Studies of Membrane Proteins - ACS Publications
Mar 30, 2025 - 8:00 PM - by nmrlearner
nmrlearner's Avatar Lipid Nanodiscs for High-Resolution NMR Studies of Membrane Proteins - ACS Publications

Lipid Nanodiscs for High-Resolution NMR Studies of Membrane Proteins ACS Publications Read here
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Using NMR Spectroscopy To Measure Protein Binding Capacity on Gold Nanoparticles - ACS Publications
Mar 30, 2025 - 8:00 PM - by nmrlearner
nmrlearner's Avatar Using NMR Spectroscopy To Measure Protein Binding Capacity on Gold Nanoparticles - ACS Publications

Using NMR Spectroscopy To Measure Protein Binding Capacity on Gold Nanoparticles ACS Publications Read here
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Protein-Ligand NOE Matching: A High-Throughput Method for Binding Pose Evaluation That Does Not Require Protein NMR Resonance Assignments - ACS Publications
Mar 30, 2025 - 8:00 PM - by nmrlearner
nmrlearner's Avatar Protein-Ligand NOE Matching: A High-Throughput Method for Binding Pose Evaluation That Does Not Require Protein NMR Resonance Assignments - ACS Publications

Protein-Ligand NOE Matching: A High-Throughput Method for Binding Pose Evaluation That Does Not Require Protein NMR Resonance Assignments ACS Publications Read here
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Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints
Mar 29, 2025 - 10:20 AM - by nmrlearner
nmrlearner's Avatar Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints

Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the determination of protein structure. The key structural information is obtained in the form of spectral cross peaks between spatially close nuclear spins, but assigning these cross peaks unambiguously to unique spin pairs is often a tedious task because of spectral overlap. Here, we use a seven-helical membrane protein Anabaena Sensory Rhodopsin (ASR) as a model system to demonstrate that transverse Paramagnetic Relaxation Enhancements (PRE) extracted from 2D MAS NMR spectra could be used to obtain a protein structural model. Starting with near complete assignments (93%) of ASR residues, TALOSâ??+â??predicted backbone dihedral angles and secondary structure restraints in the form of backbone hydrogen bonds are combined with PRE-based restraints and used to generate a coarse model. This model is subsequently utilized as a template reference to facilitate automated assignments of highly ambiguous internuclear correlations. The template is used in an iterative cross peak assignment process and is progressively improved through the inclusion of disambiguated restraints, thereby converging to a low root-mean-square-deviation structural model. In addition to improving structure calculation conversion, the inclusion of PREs also improves packing between helices within an alpha-helical bundle.



Source: Journal of Biomolecular NMR
0 Replies | 45 Views
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