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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password [NMR paper] Nanosecond Molecular Motion in pHP1alpha Liquid-Liquid Phase Separation Captured by Solid-State NMR Jan 23, 2025 - 5:30 PM - by nmrlearner Nanosecond Molecular Motion in pHP1alpha Liquid-Liquid Phase Separation Captured by Solid-State NMR The relationship among protein structure, function, and dynamics is fundamental to biological activity, particularly in more complex biomolecular systems. Solid-state and solution-state NMR techniques offer powerful means to probe these dynamics across various time scales. However, standard assumptions about molecular motion are often challenged in phase-separated systems like phosphorylated heterochromatin protein 1 alpha (pHP1?), which exhibit both solid- and solution-like characteristics.... More... 0 Replies | 108 Views 15N-detected TROSY for 1H-15N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality Jan 22, 2025 - 10:44 PM - by nmrlearner 15N-detected TROSY for 1H-15N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality Abstract Intrinsically disordered proteins and protein regions are central to many biological processes but difficult to characterize at atomic resolution. Nuclear magnetic resonance is particularly well-suited for providing structural and dynamical information on intrinsically disordered proteins, but existing NMR methodologies need to be constantly refined to provide greater sensitivity and resolution, particularly to capitalise on the potential of high magnetic fields to investigate large proteins. In this paper, we describe how 15N-detected 2D NMR experiments can be optimised for better performance. We show that using selective aliphatic 1H decoupling in N-TROSY type experiments results in significant increases in sensitivity and resolution for a prototypical intrinsically disordered protein, α-synuclein, as well as for a heterogeneous intrinsically disordered region of a large multidomain protein, CBP-ID4. We also investigated the performance of incorporating longitudinal relaxation enhancement in N-TROSY experiments, both with and without aliphatic 1H decoupling, and discussed the findings in light of the available information for the two systems. Source: Journal of Biomolecular NMR 0 Replies | 79 Views Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30Â*kDa membrane protein GlpG Jan 22, 2025 - 10:44 PM - by nmrlearner Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30Â*kDa membrane protein GlpG Abstract Chemical shift assignments of large membrane proteins by solid-state NMR experiments are challenging. Recent advancements in sensitivity-enhanced pulse sequences, have made it feasible to acquire 1H-detected 4D spectra of these challenging protein samples within reasonable timeframes. However, obtaining unambiguous assignments remains difficult without access to side-chain chemical shifts. Drawing inspiration from sensitivity-enhanced TOCSY experiments in solution NMR, we have explored the potential of 13C- 13C TOCSY mixing as a viable option for triple sensitivity-enhanced 4D experiments aimed at side-chain assignments in solid-state NMR. Through simulations and experimental trials, we have identified optimal conditions to achieve uniform transfer efficiency for both transverse components and to minimize undesired cross-transfers. Our experiments, conducted on the 30Â*kDa membrane protein GlpG embedded in E. coli liposomes, have demonstrated enhanced sensitivity compared to the most effective dipolar and J-coupling-based 13C- 13C mixing sequences. Notably, a non-uniformly sampled 4D hCXCANH spectrum with exceptionally high sensitivity was obtained in just a few days using a 600Â*MHz spectrometer equipped with a 1.3Â*mm probe operating at a magic angle spinning rate of 55Â*kHz. ... [Read More] 0 Replies | 14 Views [NMR paper] Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG Jan 22, 2025 - 10:44 PM - by nmrlearner Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG Chemical shift assignments of large membrane proteins by solid-state NMR experiments are challenging. Recent advancements in sensitivity-enhanced pulse sequences, have made it feasible to acquire ¹H-detected 4D spectra of these challenging protein samples within reasonable timeframes. However, obtaining unambiguous assignments remains difficult without access to side-chain chemical shifts. Drawing inspiration from sensitivity-enhanced TOCSY experiments in solution NMR, we have explored the... More... 0 Replies | 20 Views [NMR paper] Solution NMR goes big: Atomic resolution studies of protein components of molecular machines and phase-separated condensates Jan 22, 2025 - 10:38 AM - by nmrlearner Solution NMR goes big: Atomic resolution studies of protein components of molecular machines and phase-separated condensates The tools of structural biology have undergone remarkable advances in the past decade. These include new computational and experimental approaches that have enabled studies at a level of detail - and ease - that were not previously possible. Yet, significant deficiencies in our understanding of biomolecular function remain and new challenges must be overcome to go beyond static pictures towards a description of function in terms of structural dynamics. Solution Nuclear Magnetic Resonance (NMR)... More... 0 Replies | 45 Views [NMR paper] Backbone and Sidechain (1) H, (15) N and (13) C Resonance Assignments of a Multidrug Efflux Membrane Protein using Solution and Solid-State NMR Jan 21, 2025 - 4:06 AM - by nmrlearner Backbone and Sidechain (1) H, (15) N and (13) C Resonance Assignments of a Multidrug Efflux Membrane Protein using Solution and Solid-State NMR EmrE is a bacterial membrane-embedded multidrug transporter that functions as an asymmetric homodimer. EmrE is implicated in antibiotic resistance, but is now known to confer either resistance or susceptibility depending on the identity of the small molecule substrate. Here, we report both solution- and solid-state NMR assignments of S64V-EmrE at pH 5.8, below the pKa of critical residues E14 and H110. This includes ¹ H, ^(15) N, and ^(13) C resonance assignments of the backbone, methyl groups... More... 0 Replies | 115 Views [NMR paper] Modulation of the Oxygenation State and Intracellular pH of Erythrocytes by Inositol-Trispyrophosphate Investigated by (31)P NMR Study of 2,3-DPG Jan 21, 2025 - 4:06 AM - by nmrlearner Modulation of the Oxygenation State and Intracellular pH of Erythrocytes by Inositol-Trispyrophosphate Investigated by (31)P NMR Study of 2,3-DPG The hypoxic microenvironment is crucial for tumour cell growth and invasiveness. Tumour tissue results from adaptation to reduced oxygen availability. Hypoxia first activates pro-angiogenic signals for alleviation. Pathologic, tumour angiogenesis maintains hypoxia, impairing treatment outcomes. Vessel normalisation requires physioxia. Oxygen delivery by red blood cell (RBC) carrying haemoglobin (Hb) is enhanced by myo-inositol trispyrophosphate (ITPP), an effector of oxygen transport by RBCs.... More... 0 Replies | 24 Views Protein–ice interaction of an antifreeze protein observed with solid-state NMR - pnas.org Jan 19, 2025 - 8:54 PM - by nmrlearner Protein–ice interaction of an antifreeze protein observed with solid-state NMR - pnas.org Protein–ice interaction of an antifreeze protein observed with solid-state NMR pnas.org Read here 0 Replies | 25 Views Page 1 of 100 1 2 3 11 51 > Last »

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