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(S)Pinning down protein interactions by NMR - Teilum - 2017 - Protein Science - Wiley Online Library
Apr 03, 2025 - 10:27 AM - by nmrlearner
nmrlearner's Avatar (S)Pinning down protein interactions by NMR - Teilum - 2017 - Protein Science - Wiley Online Library

(S)Pinning down protein interactions by NMR - Teilum - 2017 - Protein Science Wiley Online Library Read here
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NMR-Based Determination of the 3D Structure of the Ligand–Protein Interaction Site without Protein Resonance Assignment - ACS Publications
Apr 03, 2025 - 10:27 AM - by nmrlearner
nmrlearner's Avatar NMR-Based Determination of the 3D Structure of the Ligand–Protein Interaction Site without Protein Resonance Assignment - ACS Publications

NMR-Based Determination of the 3D Structure of the Ligand–Protein Interaction Site without Protein Resonance Assignment ACS Publications Read here
0 Replies | 5 Views
Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin? - ACS Publications
Apr 03, 2025 - 10:27 AM - by nmrlearner
nmrlearner's Avatar Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin? - ACS Publications

Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin? ACS Publications Read here
0 Replies | 6 Views
[NMR paper] NMR characterisation of the antibiotic resistance-mediating 32mer RNA from the 23S ribosomal RNA
Apr 03, 2025 - 10:27 AM - by nmrlearner
nmrlearner's Avatar NMR characterisation of the antibiotic resistance-mediating 32mer RNA from the 23S ribosomal RNA

The increasing prevalence of antibiotic resistance represents a significant public health concern, underscoring the urgent need for the development of novel therapeutic strategies. The antibiotic effects of macrolides, the second most widely used class of antibiotics, are counteracted by Erm proteins through the methylation of adenosine 2058 of the 23S ribosomal RNA (rRNA) (~ 2900 nucleotides), yielding either monomethylated or dimethylated A2058. This methylation is the molecular basis for...

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0 Replies | 32 Views
Solid-State NMR Approaches to Internal Dynamics of Proteins: From Picoseconds to Microseconds and Seconds - ACS Publications
Apr 02, 2025 - 10:22 PM - by nmrlearner
nmrlearner's Avatar Solid-State NMR Approaches to Internal Dynamics of Proteins: From Picoseconds to Microseconds and Seconds - ACS Publications

Solid-State NMR Approaches to Internal Dynamics of Proteins: From Picoseconds to Microseconds and Seconds ACS Publications Read here
0 Replies | 18 Views
[NMR paper] Complete assignment of (1)H and (13)C NMR signals of monoglucosylated high-mannose type glycan attached to asparagine
Apr 02, 2025 - 8:39 AM - by nmrlearner
nmrlearner's Avatar Complete assignment of (1)H and (13)C NMR signals of monoglucosylated high-mannose type glycan attached to asparagine

Glc(1)Man(9)GlcNAc(2) (G1M9) glycan and other high mannose-type glycans play key roles in the quality control mechanisms of glycoprotein synthesis. The lectin-like proteins calnexin (CNX) and calreticulin (CRT) specifically recognize G1M9 glycan and assist newly synthesized glycoproteins to achieving correct folding. Nuclear magnetic resonance (NMR) spectroscopy is a unique method for analyzing the conformation, dynamics and interactions of glycans like G1M9 glycan and CNX/CRT. Accurate...

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[NMR paper] Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases
Apr 02, 2025 - 8:39 AM - by nmrlearner
nmrlearner's Avatar Genetic Encoding of Fluorinated Analogues of Phenylalanine for (19)F NMR Spectroscopy: Detection of Conformational Heterogeneity in Flaviviral NS2B-NS3 Proteases

Substituting a single hydrogen atom in a protein by fluorine provides a probe for site-specific sensing by ^(19)F nuclear magnetic resonance (NMR) spectroscopy with minimal impact on the properties of the protein. Genetic encoding systems are presented for five different fluorinated analogues of phenylalanine: 2-, 3-, 4-fluorophenylalanine, 2,6-difluorophenylalanine, and 3,5-difluorophenylalanine. The systems allow the installation of each of these amino acids with high fidelity during in vivo...

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[NMR paper] Detection of liver mitochondrial oxaloacetate by NMR spectroscopy and membrane potential-dependent accumulation
Apr 01, 2025 - 8:23 PM - by nmrlearner
nmrlearner's Avatar Detection of liver mitochondrial oxaloacetate by NMR spectroscopy and membrane potential-dependent accumulation

Oxaloacetate (OAA) is a central liver metabolite fundamental to critical metabolic pathways. However, understanding OAA metabolism in the liver has been limited because the compound is very difficult to measure by mass spectroscopy and not abundant enough for detection by other methods. Here we describe a novel approach to quantifying OAA in liver mitochondria. Moreover, we provide evidence for membrane potential-dependent OAA accumulation in mitochondria during complex II-energized respiration...

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