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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression Mar 30, 2024 - 9:30 PM - by nmrlearner Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression Abstract In NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high field-shifted resonance, which was assigned by mutational studies to the â??toggle switch tryptophanâ??. Residue-selective labeling thus enables site-specific investigations of this functionally important residue. The method described here is inexpensive, requires minimal genetic manipulation and can be expected to be applicable for yeast expression of GPCRs at large. Source: Journal of Biomolecular NMR 0 Replies | 512 Views All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy - Nature.com Mar 30, 2024 - 9:22 AM - by nmrlearner All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy - Nature.com All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy Nature.com Read here 0 Replies | 91 Views Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy - Nature.com Mar 30, 2024 - 9:22 AM - by nmrlearner Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy - Nature.com Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy Nature.com Read here 0 Replies | 81 Views Protein structure determination in living cells by in-cell NMR spectroscopy - Nature.com Mar 30, 2024 - 9:22 AM - by nmrlearner Protein structure determination in living cells by in-cell NMR spectroscopy - Nature.com Protein structure determination in living cells by in-cell NMR spectroscopy Nature.com Read here 0 Replies | 84 Views Expanding the membrane-protein NMR toolkit - Nature.com Mar 30, 2024 - 9:22 AM - by nmrlearner Expanding the membrane-protein NMR toolkit - Nature.com Expanding the membrane-protein NMR toolkit Nature.com Read here 0 Replies | 105 Views [NMR paper] Dimerization of the beta-Hairpin Membrane-Active Cationic Antimicrobial Peptide Capitellacin from Marine Polychaeta: An NMR Structural and Thermodynamic Study Mar 29, 2024 - 7:50 PM - by nmrlearner Dimerization of the beta-Hairpin Membrane-Active Cationic Antimicrobial Peptide Capitellacin from Marine Polychaeta: An NMR Structural and Thermodynamic Study Capitellacin is the ?-hairpin membrane-active cationic antimicrobial peptide from the marine polychaeta Capitella teleta. Capitellacin exhibits antibacterial activity, including against drug-resistant strains. To gain insight into the mechanism of capitellacin action, we investigated the structure of the peptide in the membrane-mimicking environment of dodecylphosphocholine (DPC) micelles using high-resolution NMR spectroscopy. In DPC solution, two structural forms of capitellacin were observed:... More... 0 Replies | 180 Views [NMR paper] Elucidation of the mechanisms of inter-domain coupling in the monomeric state of Enzyme I by high-pressure NMR Mar 29, 2024 - 7:43 AM - by nmrlearner Elucidation of the mechanisms of inter-domain coupling in the monomeric state of Enzyme I by high-pressure NMR The catalytic cycle of Enzyme I (EI), a phosphotransferase enzyme responsible for converting phosphoenolpyruvate (PEP) into pyruvate, is characterized by a series of local and global conformational rearrangements. This multistep process includes a monomer-to-dimer transition, followed by an open-to-closed rearrangement of the dimeric complex upon PEP binding. In the present study, we investigate the thermodynamics of EI dimerization using a range of high-pressure solution NMR techniques... More... 0 Replies | 108 Views [NMR paper] Metabolomic response to acute resistance exercise in healthy older adults by 1H-NMR Mar 29, 2024 - 7:43 AM - by nmrlearner Metabolomic response to acute resistance exercise in healthy older adults by 1H-NMR CONCLUSIONS: This study demonstrates that while many exercise-responsive metabolites change similarly in young and older adults, several demonstrate age-dependent changes even in the absence of evidence of sarcopenia or frailty. More... 0 Replies | 99 Views Page 8 of 100 « First < 6 7 8 9 10 18 58 > Last »

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