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Relationships Between Disease Severity and the C-reactive Protein ... - Cureus
Aug 26, 2023 - 9:35 PM - by nmrlearner
nmrlearner's Avatar Relationships Between Disease Severity and the C-reactive Protein ... - Cureus

Relationships Between Disease Severity and the C-reactive Protein ... Cureus Read here
0 Replies | 214 Views
Relationships Between Disease Severity and the C-reactive Protein ... - Cureus
Aug 25, 2023 - 2:40 PM - by nmrlearner
nmrlearner's Avatar Relationships Between Disease Severity and the C-reactive Protein ... - Cureus

Relationships Between Disease Severity and the C-reactive Protein ... Cureus Read here
0 Replies | 204 Views
[NMR paper] Illuminating GPCR signaling mechanisms by NMR spectroscopy with stable-isotope labeled receptors
Aug 25, 2023 - 2:40 PM - by nmrlearner
nmrlearner's Avatar Illuminating GPCR signaling mechanisms by NMR spectroscopy with stable-isotope labeled receptors

G protein-coupled receptors (GPCRs) exhibit remarkable structural plasticity, which underlies their capacity to recognize a wide range of extracellular molecules and interact with intracellular partner proteins. Nuclear magnetic resonance (NMR) spectroscopy is uniquely well-suited to investigate GPCR structural plasticity, enabled by stable-isotope "probes" incorporated into receptors that inform on structure and dynamics. Progress with stable-isotope labeling methods in Eukaryotic expression...

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0 Replies | 242 Views
[NMR paper] Discerning conformational dynamics and binding kinetics of GPCRs by 19F NMR
Aug 25, 2023 - 2:40 PM - by nmrlearner
nmrlearner's Avatar Discerning conformational dynamics and binding kinetics of GPCRs by 19F NMR

^(19)F NMR provides a way of monitoring conformational dynamics of G-protein coupled receptors (GPCRs) from the perspective of an ensemble. While X-ray crystallography provides exquisitely resolved high-resolution structures of specific states, it generally does not recapitulate the true ensemble of functional states. Fluorine (^(19)F) NMR provides a highly sensitive spectroscopic window into the conformational ensemble, generally permitting the direct quantification of resolvable states....

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0 Replies | 135 Views
[NMR paper] Fluoride permeation mechanism of the Fluc channel in liposomes revealed by solid-state NMR
Aug 25, 2023 - 2:40 PM - by nmrlearner
nmrlearner's Avatar Fluoride permeation mechanism of the Fluc channel in liposomes revealed by solid-state NMR

Solid-state nuclear magnetic resonance (ssNMR) methods can probe the motions of membrane proteins in liposomes at the atomic level and propel the understanding of biomolecular processes for which static structures cannot provide a satisfactory description. In this work, we report our study on the fluoride channel Fluc-Ec1 in phospholipid bilayers based on ssNMR and molecular dynamics simulations. Previously unidentified fluoride binding sites in the aqueous vestibules were experimentally...

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0 Replies | 188 Views
SARS-CoV-2: The grasping fingers of the viral N protein - EurekAlert
Aug 23, 2023 - 10:11 AM - by nmrlearner
nmrlearner's Avatar SARS-CoV-2: The grasping fingers of the viral N protein EurekAlert
SARS-CoV-2: The grasping fingers of the viral N protein - EurekAlert
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0 Replies | 191 Views
Protein Crystallization And Crystallography Market: Segmented: Outlook 2030: Industry Insights & Opportunity Evaluation, 2019-2030 - Yahoo Finance
Aug 23, 2023 - 10:11 AM - by nmrlearner
nmrlearner's Avatar Protein Crystallization And Crystallography Market: Segmented: Outlook 2030: Industry Insights & Opportunity Evaluation, 2019-2030 Yahoo Finance
Protein Crystallization And Crystallography Market: Segmented: Outlook 2030: Industry Insights & Opportunity Evaluation, 2019-2030 - Yahoo Finance
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0 Replies | 201 Views
[NMR paper] Rapid Determination of the Topology of Oligomeric ?-Helical Membrane Proteins by Water- and Lipid-Edited Methyl NMR
Aug 23, 2023 - 10:11 AM - by nmrlearner
nmrlearner's Avatar Rapid Determination of the Topology of Oligomeric ?-Helical Membrane Proteins by Water- and Lipid-Edited Methyl NMR

Single-span oligomeric ?-helical transmembrane proteins are common in virus ion channels, which are targets of antiviral drugs. Knowledge about the high-resolution structures of these oligomeric ?-helical bundles is so far scarce. Structure determination of these membrane proteins by solid-state NMR traditionally requires resolving and assigning protein chemical shifts and measuring many interhelical distances, which are time-consuming. To accelerate experimental structure determination, here we...

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0 Replies | 338 Views
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