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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins Sep 10, 2023 - 2:39 AM - by nmrlearner A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins Abstract Introducing the flow through the bioreactor has revolutionized in-cell NMR spectroscopy by prolonging the measurement time available to acquire spectral information about biomacromolecules in metabolically active cells. Bioreactor technology relies on immobilizer matrices, which secure cells in the active volume of the NMR coil and enable uniform perfusion of the growth medium, supplying fresh nutrients to the cells while removing toxic byproducts of their metabolism. The main drawbacks of commonly used matrices include the inability to recover intact cells post-measurement for additional analyses and/or requirements for specific operating temperatures. Here, we report on the development and characterization of a set of thermosensitive and nontoxic triblock copolymers based on poly(D,L-lactide)-b-poly(ethylene glycol)-b-poly(D,L-lactide) (PLA-PEG-PLA). Here, we show for the first time that these copolymers are suitable as immobilizer matrices for the acquisition of in-cell NMR spectra of nucleic acids and proteins over a commonly used sample temperature range of 15â??40Â*°C and, importantly, allow recovery of cells after completion of in-cell NMR spectra acquisition. We compared the performances of currently used matrices in terms of cell viability (dye exclusion assays), cellular metabolism (1D 31P NMR), and quality of in-cell NMR spectra of two model biomacromolecules (hybrid double-stranded/i-motif DNA and ubiquitin). Our results demonstrate the suitability and advantages of PLA-PEG-PLA copolymers for application in bioreactor-assisted in-cell NMR. ... [Read More] 0 Replies | 834 Views Postdoctoral Scholar – Structural biology, NMR, protein chemistry ... - American Chemical Society Sep 10, 2023 - 2:39 AM - by nmrlearner Postdoctoral Scholar – Structural biology, NMR, protein chemistry ... - American Chemical Society Postdoctoral Scholar – Structural biology, NMR, protein chemistry ... American Chemical Society Read here 0 Replies | 225 Views [NMR paper] A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins Sep 10, 2023 - 2:39 AM - by nmrlearner A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins Introducing the flow through the bioreactor has revolutionized in-cell NMR spectroscopy by prolonging the measurement time available to acquire spectral information about biomacromolecules in metabolically active cells. Bioreactor technology relies on immobilizer matrices, which secure cells in the active volume of the NMR coil and enable uniform perfusion of the growth medium, supplying fresh nutrients to the cells while removing toxic byproducts of their metabolism. The main drawbacks of... More... 0 Replies | 177 Views HIV-1 Vpu protein forms stable oligomers in aqueous solution via its ... - Nature.com Sep 08, 2023 - 8:28 PM - by nmrlearner HIV-1 Vpu protein forms stable oligomers in aqueous solution via its ... - Nature.com HIV-1 Vpu protein forms stable oligomers in aqueous solution via its ... Nature.com Read here 0 Replies | 245 Views [NMR paper] Detection of Unfolded Cellular Proteins Using Nanochannel Array with Probe-Functionalized Outer-Surfaces Sep 08, 2023 - 8:28 PM - by nmrlearner Detection of Unfolded Cellular Proteins Using Nanochannel Array with Probe-Functionalized Outer-Surfaces Angewandte Chemie International Edition, Accepted Article. More... 0 Replies | 293 Views [NMR paper] Elongated Bacterial Pili as a Versatile Alignment Medium for NMR Spectroscopy Sep 07, 2023 - 12:54 AM - by nmrlearner Elongated Bacterial Pili as a Versatile Alignment Medium for NMR Spectroscopy Angewandte Chemie International Edition, Volume 62, Issue 37, September 11, 2023. More... 0 Replies | 293 Views [NMR paper] Methods for Structure Determination of SH2 Domain-Phosphopeptide Complexes by NMR Sep 06, 2023 - 1:37 AM - by nmrlearner Methods for Structure Determination of SH2 Domain-Phosphopeptide Complexes by NMR Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique to solve the structure of biomolecular complexes at atomic resolution in solution. Small proteins such as Src-homology 2 (SH2) domains have fast tumbling rates and long-lived NMR signals, making them particularly suited to be studied by standard NMR methods. SH2 domains are modular proteins whose function is the recognition of sequences containing phosphotyrosines. In this chapter, we describe the application of NMR to assess... More... 0 Replies | 201 Views [NMR paper] NMR assignment and dynamics of the dimeric form of soluble C-terminal domain major ampullate spidroin 2 from Latrodectus hesperus Sep 06, 2023 - 1:37 AM - by nmrlearner NMR assignment and dynamics of the dimeric form of soluble C-terminal domain major ampullate spidroin 2 from Latrodectus hesperus Spider dragline silk has attracted great interest due to its outstanding mechanical properties, which exceed those of man-made synthetic materials. Dragline silk, which is composed of at least major ampullate spider silk protein 1 and 2 (MaSp1 and MaSp2), contains a long repetitive domain flanked by N-terminal and C-terminal domains (NTD and CTD). Despite the small size of the CTD, this domain plays a crucial role as a molecular switch that regulates and directs spider silk self-assembly. In... More... 0 Replies | 250 Views Page 65 of 100 « First < 15 55 63 64 65 66 67 75 > Last »

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