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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password Visualizing Side Chains of Invisible Protein Conformers by Solution NMR - ScienceDirect.com Apr 08, 2024 - 3:06 PM - by nmrlearner Visualizing Side Chains of Invisible Protein Conformers by Solution NMR - ScienceDirect.com Visualizing Side Chains of Invisible Protein Conformers by Solution NMR ScienceDirect.com Read here 0 Replies | 101 Views [NMR paper] Application of an NMR/crystallography fragment screening platform for the assessment and rapid discovery of new HIV-CA binding fragments Apr 08, 2024 - 3:06 PM - by nmrlearner Application of an NMR/crystallography fragment screening platform for the assessment and rapid discovery of new HIV-CA binding fragments Identification and assessment of novel targets is essential to combat drug resistance in the treatment of HIV/AIDS. HIV Capsid (HIV-CA), the protein playing a major role in both the early and late stages of the viral life cycle, has emerged as an important target. We have applied an NMR fragment screening platform and identified molecules that bind to the N-terminal domain (NTD) of HIV-CA at a site close to the interface with the C-terminal domain (CTD). Using X-ray crystallography, we have been... More... 0 Replies | 234 Views [NMR paper] Assessment of monoclonal antibody glycosylation: a comparative study using HRMS, NMR, and HILIC-FLD Apr 08, 2024 - 3:06 PM - by nmrlearner Assessment of monoclonal antibody glycosylation: a comparative study using HRMS, NMR, and HILIC-FLD Monoclonal antibodies (mAbs) represent the largest class of therapeutic protein drug products. mAb glycosylation produces a heterogeneous, analytically challenging distribution of glycoforms that typically should be adequately characterized because glycosylation-based product quality attributes (PQAs) can impact product quality, immunogenicity, and efficacy. In this study, two products were compared using a panel of analytical methods. Two high-resolution mass spectrometry (HRMS) workflows were... More... 0 Replies | 55 Views [NMR paper] Protocol to identify the ligand binding site of Mincle using NMR spectroscopy Apr 08, 2024 - 3:06 PM - by nmrlearner Protocol to identify the ligand binding site of Mincle using NMR spectroscopy Mincle (macrophage-inducible C-type lectin, CLEC4E) is a C-type lectin immune-stimulatory receptor that can be targeted for inducing potent adjuvant effects. Mincle can recognize trehalose dimycolate and related glycolipids. Here, we present a protocol to identify the ligand binding mode of Mincle. We describe steps for preparing labeled Mincle ectodomain, data acquisition, and analysis of nuclear magnetic resonance experiments using non-detergent sulfobetaine-195. This protocol can be applied... More... 0 Replies | 52 Views [NMR paper] Real-Time Monitoring of RAS Activity Using In Vitro and In-Cell NMR Spectroscopy Apr 08, 2024 - 3:06 PM - by nmrlearner Real-Time Monitoring of RAS Activity Using In Vitro and In-Cell NMR Spectroscopy The activation level of RAS can be determined by GTP hydrolysis rate (k(hy)) and GDP-GTP exchange rates (k(ex)). Either impaired GTP hydrolysis or enhanced GDP-GTP exchange causes the aberrant activation of RAS in oncogenic mutants. Therefore, it is important to quantify the k(hy) and k(ex) for understanding the mechanisms of RAS oncogenesis and drug development. Conventional methods have individually measured the k(ex) and k(hy) of RAS. However, within the intracellular environment, GTP... More... 0 Replies | 51 Views [NMR paper] Profiling Complex RAS-Effector Interactions Using NMR Spectroscopy Apr 08, 2024 - 3:06 PM - by nmrlearner Profiling Complex RAS-Effector Interactions Using NMR Spectroscopy Knowledge of how effectors interact with RAS GTPases is key to understanding how these switch-like proteins function in cells. Effectors bind specifically to GTP-loaded RAS using RAS association (RA) or RAS binding domains (RBDs) that show wide-ranging affinities and thermodynamic characteristics. Both normal development and RAS-induced tumorigenesis depend on multiple distinct effector proteins that are frequently co-expressed and co-localized, suggesting an antagonistic nature to signaling... More... 0 Replies | 53 Views [NMR paper] 1D and 2D NMR for KRAS:Ligand Binding Apr 08, 2024 - 3:06 PM - by nmrlearner 1D and 2D NMR for KRAS:Ligand Binding Fragment-based screening by ligand-observed 1D NMR and binding interface mapping by protein-observed 2D NMR are popular methods used in drug discovery. These methods allow researchers to detect compound binding over a wide range of affinities and offer a simultaneous assessment of solubility, purity, and chemical formula accuracy of the target compounds and the ^(15)N-labeled protein when examined by 1D and 2D NMR, respectively. These methods can be applied for screening fragment binding to the... More... 0 Replies | 55 Views [NMR paper] Predicting the Stability of Lyophilized Human Serum Albumin Formulations Containing Sucrose and Trehalose Using Solid-State NMR Spectroscopy: Effect of Storage Temperature on (1)H T(1) Relaxation Times Apr 08, 2024 - 3:06 PM - by nmrlearner Predicting the Stability of Lyophilized Human Serum Albumin Formulations Containing Sucrose and Trehalose Using Solid-State NMR Spectroscopy: Effect of Storage Temperature on (1)H T(1) Relaxation Times In a lyophilized protein/disaccharide system, the ability of the disaccharide to form a homogeneous mixture with the protein and to slow the protein mobility dictates the stabilization potential of the formulation. Human serum albumin was lyophilized with sucrose or trehalose in histidine, phosphate, or citrate buffer. ¹H T(1) relaxation times were measured by solid-state NMR spectroscopy and were used to assess the homogeneity and mobility of the samples after zero, six, and twelve months at... 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