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[NMR paper] A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity
Sep 22, 2023 - 10:23 PM - by nmrlearner
nmrlearner's Avatar A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity

Studying the structural aspects of proteins within sub-cellular compartments is of growing interest. Dynamic nuclear polarization supported solid-state NMR (DNP-ssNMR) is uniquely suited to provide such information, but critically lacks the desired sensitivity and resolution. Here we utilize SNAPol-1, a novel biradical, to conduct DNP-ssNMR at high-magnetic fields (800 MHz/527 GHz) inside HeLa cells and isolated cell nuclei electroporated with [^(13)C,^(15)N] labeled ubiquitin. We report that...

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0 Replies | 496 Views
[NMR paper] NMR chemical shift prediction and structural elucidation of linker-containing oligo- and polysaccharides using the computer program CASPER
Sep 22, 2023 - 10:23 PM - by nmrlearner
nmrlearner's Avatar NMR chemical shift prediction and structural elucidation of linker-containing oligo- and polysaccharides using the computer program CASPER

Carbohydrate structures containing alkyl groups as aglycones are useful for investigating enzyme activity and glycan-protein interactions. Moreover, linker-containing oligosaccharides with a spacer group are commonly used to print glycan microarrays or to prepare protein-conjugates as vaccine candidates. The structural accuracy of these synthesized glycans are essential for interpretation of results from biological experiments in which the compounds have been used and NMR spectroscopy can...

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0 Replies | 158 Views
[NMR paper] Interactions of human mitochondrial Ferredoxin 1 (Adrenodoxin) by NMR; modulation by cytochrome P450 substrate and by truncation of the C-terminal tail
Sep 22, 2023 - 10:23 PM - by nmrlearner
nmrlearner's Avatar Interactions of human mitochondrial Ferredoxin 1 (Adrenodoxin) by NMR; modulation by cytochrome P450 substrate and by truncation of the C-terminal tail

Human Ferredoxin 1, also referred to as Adrenodoxin (Adx), is the sole electron carrier supporting the function of all seven mitochondrial cytochrome P450 (CYP) enzymes. Adx utilizes conserved negatively charged residues along its ?-helix3 to interact with either the proximal surface of CYP enzymes or the binding surface of Adrendodoxin Reductase (AdR). However, in the oxidized state, Adx assumes a monomer-homodimer equilibrium that requires the presence of its unstructured C-terminal tail....

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0 Replies | 179 Views
Google DeepMind's protein-predicting AlphaFold, OCT imaging ... - FierceBiotech
Sep 21, 2023 - 8:10 PM - by nmrlearner
nmrlearner's Avatar Google DeepMind's protein-predicting AlphaFold, OCT imaging ... FierceBiotech
Google DeepMind's protein-predicting AlphaFold, OCT imaging ... - FierceBiotech
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0 Replies | 178 Views
Protein Characterization and Identification Market Size Envisions ... - InvestorsObserver
Sep 21, 2023 - 3:14 AM - by nmrlearner
nmrlearner's Avatar Protein Characterization and Identification Market Size Envisions ... InvestorsObserver
Protein Characterization and Identification Market Size Envisions ... - InvestorsObserver
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0 Replies | 171 Views
Protein Characterization and Identification Market Size Envisions ... - GlobeNewswire
Sep 19, 2023 - 8:52 PM - by nmrlearner
nmrlearner's Avatar Protein Characterization and Identification Market Size Envisions ... - GlobeNewswire

Protein Characterization and Identification Market Size Envisions ... GlobeNewswire Read here
0 Replies | 165 Views
[NMR paper] Probing the Conformational Space of the Cannabinoid Receptor 2 and a Systematic Investigation of DNP-Enhanced MAS NMR Spectroscopy of Proteins in Detergent Micelles
Sep 19, 2023 - 8:52 PM - by nmrlearner
nmrlearner's Avatar Probing the Conformational Space of the Cannabinoid Receptor 2 and a Systematic Investigation of DNP-Enhanced MAS NMR Spectroscopy of Proteins in Detergent Micelles

Tremendous progress has been made in determining the structures of G-protein coupled receptors (GPCR) and their complexes in recent years. However, understanding activation and signaling in GPCRs is still challenging due to the role of protein dynamics in these processes. Here, we show how dynamic nuclear polarization (DNP)-enhanced magic angle spinning nuclear magnetic resonance in combination with a unique pair labeling approach can be used to study the conformational ensemble at specific...

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0 Replies | 381 Views
Mixed, nonclassical behavior in a classic allosteric protein ... - pnas.org
Sep 15, 2023 - 1:33 AM - by nmrlearner
nmrlearner's Avatar Mixed, nonclassical behavior in a classic allosteric protein ... - pnas.org

Mixed, nonclassical behavior in a classic allosteric protein ... pnas.org Read here
0 Replies | 207 Views
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