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Biochemistry Seminar: Melanie Cocco, “Protein Dynamics ... - City University of New York
Oct 18, 2023 - 6:17 AM - by nmrlearner
nmrlearner's Avatar Biochemistry Seminar: Melanie Cocco, “Protein Dynamics ... - City University of New York

Biochemistry Seminar: Melanie Cocco, “Protein Dynamics ... City University of New York Read here
0 Replies | 212 Views
[NMR paper] NMR screening method based on 19F spin-spin relaxation time for analyses of fluorinated compound bound to proteins
Oct 17, 2023 - 3:24 AM - by nmrlearner
nmrlearner's Avatar NMR screening method based on 19F spin-spin relaxation time for analyses of fluorinated compound bound to proteins

NMR screening methods based on ^(19)F spin-spin relaxation time (^(19)F-T(2)) were applied to fluorinated compounds bound to human serum albumin. Diflunisal and fleroxacin (the fluorinated compounds) contain two and three fluorine atoms per molecule, respectively, and are suitable as the model system for ^(19)F NMR analysis. It was shown that ^(19)F-T(2) was more sensitive in monitoring the binding affinity to the target protein than ^(19)F spin-lattice relaxation time (^(19)F-T(1)). The...

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0 Replies | 248 Views
[NMR paper] NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes
Oct 15, 2023 - 2:43 AM - by nmrlearner
nmrlearner's Avatar NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes

In mitochondria, the major subunits of oxidative phosphorylation complexes are translated by the mitochondrial ribosome (mito-ribosome). The correct insertion and assembly of these subunits into the inner mitochondrial membrane (IMM) are facilitated by mitochondrial oxidase assembly protein 1 (Oxa1) during the translation process. This co-translational insertion process involves an association between the mito-ribosome and the C-terminus of Oxa1 (Oxa1-CTD) Nuclear magnetic resonance (NMR)...

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0 Replies | 314 Views
A Holy Grail — The Prediction of Protein Structure | NEJM - nejm.org
Oct 13, 2023 - 3:16 PM - by nmrlearner
nmrlearner's Avatar A Holy Grail — The Prediction of Protein Structure | NEJM nejm.org
A Holy Grail — The Prediction of Protein Structure | NEJM - nejm.org
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0 Replies | 207 Views
[NMR paper] High and fast: NMR protein-proton side-chain assignments at 160 kHz and 1.2 GHz
Oct 13, 2023 - 3:16 PM - by nmrlearner
nmrlearner's Avatar High and fast: NMR protein-proton side-chain assignments at 160 kHz and 1.2 GHz

The NMR spectra of side-chain protons in proteins provide important information, not only about their structure and dynamics, but also about the mechanisms that regulate interactions between macromolecules. However, in the solid-state, these resonances are particularly difficult to resolve, even in relatively small proteins. We show that magic-angle-spinning (MAS) frequencies of 160 kHz, combined with a high magnetic field of 1200 MHz proton Larmor frequency, significantly improve their spectral...

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0 Replies | 270 Views
Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics
Oct 11, 2023 - 3:50 PM - by nmrlearner
nmrlearner's Avatar Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics

Abstract

In this study, we present the synthesis and incorporation of a metabolic isoleucine precursor compound for selective methylene labeling. The utility of this novel α-ketoacid isotopologue is shown by incorporation into the protein Brd4-BD1, which regulates gene expression by binding to acetylated histones. High quality single quantum 13Câ??1Â*H-HSQC were obtained, as well as triple quantum HTQC spectra, which are superior in terms of significantly increased 13C-T2 times. Additionally, large chemical shift perturbations upon ligand binding were observed. Our study thus proves the great sensitivity of this precursor as a reporter for side-chain dynamic studies and for investigations of CH-Ď? interactions in protein-ligand complexes.



Source: Journal of Biomolecular NMR
0 Replies | 326 Views
Biochemistry Seminar: Rachel Martin, “Protein aging and cataract ... - City University of New York
Oct 11, 2023 - 3:50 PM - by nmrlearner
nmrlearner's Avatar Biochemistry Seminar: Rachel Martin, “Protein aging and cataract ... - City University of New York

Biochemistry Seminar: Rachel Martin, “Protein aging and cataract ... City University of New York Read here
0 Replies | 235 Views
Cryo-EM reveals structure of key enzyme involved in protein ... - News-Medical.Net
Oct 10, 2023 - 2:10 PM - by nmrlearner
nmrlearner's Avatar Cryo-EM reveals structure of key enzyme involved in protein ... - News-Medical.Net

Cryo-EM reveals structure of key enzyme involved in protein ... News-Medical.Net Read here
0 Replies | 240 Views
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