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Revealing structural secrets of a key cancer protein - The Ohio State University News
Oct 22, 2023 - 11:46 AM - by nmrlearner
nmrlearner's Avatar Revealing structural secrets of a key cancer protein - The Ohio State University News

Revealing structural secrets of a key cancer protein The Ohio State University News Read here
0 Replies | 273 Views
[NMR paper] Protein methylation characterization using NMR without isotopic labeling
Oct 21, 2023 - 12:03 PM - by nmrlearner
nmrlearner's Avatar Protein methylation characterization using NMR without isotopic labeling

Protein methylation is crucial in epigenetics, and targeting the involved methyltransferases shows great potential for therapeutic intervention with several inhibitors in clinical trials for oncology indications. Therefore, characterization of protein methylation is essential for understanding the methyltransferase function and discovering chemical inhibitors and antagonists. While NMR has been used to measure methylation rates, isotopic labeling of protein or methyl donors can be costly and...

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0 Replies | 270 Views
[NMR paper] Assignment of the disordered, proline-rich N-terminal domain of the tumour suppressor p53 protein using 1HN and 1H?-detected NMR measurements
Oct 21, 2023 - 12:03 PM - by nmrlearner
nmrlearner's Avatar Assignment of the disordered, proline-rich N-terminal domain of the tumour suppressor p53 protein using 1HN and 1H?-detected NMR measurements

Protein p53 is mostly known for playing a key role in tumour suppression, and mutations in the p53 gene are amongst the most frequent genomic events accompanying oncogenic transformation. Continuous research is conducted to target disordered proteins/protein regions for cancer therapy, for which atomic level information is also necessary. The disordered N-terminal part of p53 contains the transactivation and the proline-rich domains-which besides being abundant in proline residues-contains...

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0 Replies | 182 Views
[NMR paper] Solution NMR assignments and structure for the dimeric kinesin neck domain
Oct 21, 2023 - 12:03 PM - by nmrlearner
nmrlearner's Avatar Solution NMR assignments and structure for the dimeric kinesin neck domain

Kinesin is a motor protein, comprised of two heavy and two light chains that transports cargo along the cytoskeletal microtubule filament network. The heavy chain has a neck domain connecting the ATPase motor head responsible for walking along microtubules, with the stalk and subsequent tail domains that bind cargo. The neck domain consists of a coiled coli homodimer with about five heptad repeats, preceded by a linker region that joins to the ATPase head. Here we report ¹H, ^(15)N, and ^(13)C...

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0 Replies | 205 Views
Revealing structural secrets of a key cancer protein - Phys.org
Oct 20, 2023 - 12:52 PM - by nmrlearner
nmrlearner's Avatar Revealing structural secrets of a key cancer protein - Phys.org

Revealing structural secrets of a key cancer protein Phys.org Read here
0 Replies | 238 Views
[NMR paper] Drug repurposing for the identification of new Bcl-2 inhibitors: In vitro, STD-NMR, molecular docking, and dynamic simulation studies
Oct 20, 2023 - 12:52 PM - by nmrlearner
nmrlearner's Avatar Drug repurposing for the identification of new Bcl-2 inhibitors: In vitro, STD-NMR, molecular docking, and dynamic simulation studies

BACKGROUND: The anti-apoptotic protein B-Cell Lymphoma 2 (Bcl-2) is a key target for the development of anti-cancer agents, as its overexpression can render cancer cells resistant to chemotherapeutic treatments.

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0 Replies | 260 Views
[NMR paper] Fragment-based screening by protein-detected NMR spectroscopy
Oct 20, 2023 - 12:52 PM - by nmrlearner
nmrlearner's Avatar Fragment-based screening by protein-detected NMR spectroscopy

Fragment-based drug discovery (FBDD) identifies low molecular weight compounds that can be developed into ligands with high affinity and selectivity for therapeutic targets. Screening fragment libraries (
0 Replies | 178 Views
[NMR paper] Analysis of enzyme reactions using NMR techniques: A case study with ?-methylacyl-CoA racemase (AMACR)
Oct 20, 2023 - 12:52 PM - by nmrlearner
nmrlearner's Avatar Analysis of enzyme reactions using NMR techniques: A case study with ?-methylacyl-CoA racemase (AMACR)

?-Methylacyl-CoA racemase (AMACR; P504S) catalyzes the conversion of R-2-methylacyl-CoA esters into their corresponding S-2-methylacyl-CoA epimers enabling their degradation by ?-oxidation. The enzyme also catalyzes the key epimerization reaction in the pharmacological activation pathway of ibuprofen and related drugs. AMACR protein levels and enzymatic activity are increased in prostate cancer, and the enzyme is a recognized drug target. Key to the development of novel treatments based on AMACR...

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0 Replies | 186 Views
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