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[NMR paper] NMR-based metabolomics in giant cell arteritis and polymyalgia rheumatica sequential sera differentiates active and inactive disease
Nov 08, 2023 - 11:20 AM - by nmrlearner
nmrlearner's Avatar NMR-based metabolomics in giant cell arteritis and polymyalgia rheumatica sequential sera differentiates active and inactive disease

CONCLUSION: The NMR profile of serum metabolome could identify and propose sensitive biomarkers of inflammation. Metabolome alterations, following GCs treatment, could provide predictors for future steroid-induced side effects.

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0 Replies | 237 Views
[NMR paper] Real-time monitoring of the reaction of KRAS G12C mutant specific covalent inhibitor by in vitro and in-cell NMR spectroscopy
Nov 08, 2023 - 11:20 AM - by nmrlearner
nmrlearner's Avatar Real-time monitoring of the reaction of KRAS G12C mutant specific covalent inhibitor by in vitro and in-cell NMR spectroscopy

KRAS mutations are major drivers of various cancers. Recently, allele-specific inhibitors of the KRAS G12C mutant were developed that covalently modify the thiol of Cys12, thereby trapping KRAS in an inactive GDP-bound state. To study the mechanism of action of the covalent inhibitors in both in vitro and intracellular environments, we used real-time NMR to simultaneously observe GTP hydrolysis and inhibitor binding. In vitro NMR experiments showed that the rate constant of ARS-853 modification...

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0 Replies | 119 Views
Scientists Uncover Structural Secrets of Key Cancer Protein - SciTechDaily
Nov 07, 2023 - 7:59 AM - by nmrlearner
nmrlearner's Avatar Scientists Uncover Structural Secrets of Key Cancer Protein - SciTechDaily

Scientists Uncover Structural Secrets of Key Cancer Protein SciTechDaily Read here
0 Replies | 143 Views
[NMR paper] Building a Cost-Efficient High-Pressure Cell for Online High-Field NMR and MRI Using Standard Static Probe Heads: An In Situ Demonstration on Clathrate Hydrate Formation
Nov 07, 2023 - 7:59 AM - by nmrlearner
nmrlearner's Avatar Building a Cost-Efficient High-Pressure Cell for Online High-Field NMR and MRI Using Standard Static Probe Heads: An In Situ Demonstration on Clathrate Hydrate Formation

High-pressure nuclear magnetic resonance (NMR) spectroscopy finds remarkable applications in catalysis, protein biochemistry and biophysics, analytical chemistry, material science, energy, and environmental control but requires expensive probe heads and/or sample cells. This contribution describes the design, construction, and testing of a low-cost 5 mm NMR tube suitable for high-pressure NMR measurements of up to 30 MPa. The sample cell comprises a standard, 5 mm single-crystal sapphire tube...

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0 Replies | 197 Views
[NMR paper] Structural Analysis of Proteins from Bacterial Secretion Systems and Their Assemblies by NMR Spectroscopy
Nov 06, 2023 - 7:18 PM - by nmrlearner
nmrlearner's Avatar Structural Analysis of Proteins from Bacterial Secretion Systems and Their Assemblies by NMR Spectroscopy

Bacterial secretion systems are built up from proteins with different physicochemical characteristics, such as highly hydrophobic transmembrane polypeptides, and soluble periplasmic or intracellular domains. A single complex can be composed of more than ten proteins with distinct features, spreading through different cellular compartments. The membrane and multicompartment nature of the proteins, and their large molecular weight make their study challenging. However, information on their...

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0 Replies | 153 Views
[NMR paper] Streamlined Protein-Protein Interface Loop Mimicry
Nov 05, 2023 - 6:10 PM - by nmrlearner
nmrlearner's Avatar Streamlined Protein-Protein Interface Loop Mimicry


Angewandte Chemie International Edition, EarlyView.

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0 Replies | 157 Views
A viral protein targets mitochondria and chloroplasts by subverting ... - American Society for Microbiology
Nov 05, 2023 - 5:44 AM - by nmrlearner
nmrlearner's Avatar A viral protein targets mitochondria and chloroplasts by subverting ... - American Society for Microbiology

A viral protein targets mitochondria and chloroplasts by subverting ... American Society for Microbiology Read here
0 Replies | 207 Views
[NMR paper] Solid-state NMR structure determination of a membrane protein in E. coli cellular inner membrane
Nov 02, 2023 - 12:38 PM - by nmrlearner
nmrlearner's Avatar Solid-state NMR structure determination of a membrane protein in E. coli cellular inner membrane

Structure determination of membrane proteins in native cellular membranes is critical to precisely reveal their structures in physiological conditions. However, it remains challenging for solid-state nuclear magnetic resonance (ssNMR) due to the low sensitivity and high complexity of ssNMR spectra of cellular membranes. Here, we present the structure determination of aquaporin Z (AqpZ) by ssNMR in Escherichia coli inner membranes. To enhance the signal sensitivity of AqpZ, we optimized protein...

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0 Replies | 336 Views
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