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[NMR paper] Peptoid-based macrodiscs of variable lipid composition for structural studies of membrane proteins by oriented-sample solid-state NMR
Dec 15, 2023 - 5:49 AM - by nmrlearner
nmrlearner's Avatar Peptoid-based macrodiscs of variable lipid composition for structural studies of membrane proteins by oriented-sample solid-state NMR

Solid-state Nuclear Magnetic Resonance (NMR) in combination with magnetically aligned discoidal lipid mimics allows for studying the conformations of membrane proteins in planar, lipid-rich bilayer environments and at the physiological temperature. We have recently demonstrated the general applicability of macrodiscs composed of DMPC lipids and peptoid belts, which yield magnetic alignment and NMR spectroscopic resolution comparable or superior to detergent-containing bicelles. Here we report on...

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0 Replies | 387 Views
[NMR paper] Richer than previously probed: An application of 1H NMR reveals one hundred metabolites using only fifty microliter serum
Dec 14, 2023 - 10:45 AM - by nmrlearner
nmrlearner's Avatar Richer than previously probed: An application of 1H NMR reveals one hundred metabolites using only fifty microliter serum

The classical approach restricts the detection of metabolites in serum samples by using nuclear magnetic resonance (NMR) spectroscopy; however, the presence of copious proteins and lipoproteins emphasize and necessitate the development of a contemporary, high-throughput tactic. To eliminate the lipoproteins and proteins from sera to engender filtered sera (FS), the study was executed with 50 ?l serum obtained from five healthy individuals with 5 years of age difference from 25 to 45 years old...

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0 Replies | 178 Views
[NMR paper] Optimal 13C NMR investigation of intrinsically disordered proteins at 1.2 GHz
Dec 14, 2023 - 10:45 AM - by nmrlearner
nmrlearner's Avatar Optimal 13C NMR investigation of intrinsically disordered proteins at 1.2 GHz

Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for characterizing biomolecules such as proteins and nucleic acids at atomic resolution. Increased magnetic field strengths drive progress in biomolecular NMR applications, leading to improved performance, e.g., higher resolution. A new class of NMR spectrometers with a 28.2 T magnetic field (1.2 GHz ¹H frequency) has been commercially available since the end of 2019. The availability of ultra-high-field NMR instrumentation...

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0 Replies | 97 Views
[NMR paper] Enlarging the scenario of site directed 19F labeling for NMR spectroscopy of biomolecules
Dec 14, 2023 - 10:45 AM - by nmrlearner
nmrlearner's Avatar Enlarging the scenario of site directed 19F labeling for NMR spectroscopy of biomolecules

The possibility of using selectively incorporated ^(19)F nuclei for NMR spectroscopic studies has retrieved increasing interest in recent years. The high gyromagnetic ratio of ^(19)F and its absence in native biomolecular systems make this nucleus an interesting alternative to standard ¹H NMR spectroscopy. Here we show how we can attach a label, carrying a ^(19)F atom, to protein tyrosines, through the use of a specific three component Mannich-type reaction. To validate the efficacy and the...

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0 Replies | 105 Views
Exploiting conformational dynamics to modulate the function of designed proteins | Proceedings of the National ... - pnas.org
Dec 13, 2023 - 2:17 PM - by nmrlearner
nmrlearner's Avatar Exploiting conformational dynamics to modulate the function of designed proteins | Proceedings of the National ... - pnas.org

Exploiting conformational dynamics to modulate the function of designed proteins | Proceedings of the National ... pnas.org Read here
0 Replies | 85 Views
Exploiting conformational dynamics to modulate the function of designed proteins | Proceedings of the National ... - pnas.org
Dec 13, 2023 - 2:17 PM - by nmrlearner
nmrlearner's Avatar Exploiting conformational dynamics to modulate the function of designed proteins | Proceedings of the National ... - pnas.org

Exploiting conformational dynamics to modulate the function of designed proteins | Proceedings of the National ... pnas.org Read here
0 Replies | 98 Views
[NMR paper] Activation of caspase-9 on the apoptosome as studied by methyl-TROSY NMR
Dec 13, 2023 - 12:23 AM - by nmrlearner
nmrlearner's Avatar Activation of caspase-9 on the apoptosome as studied by methyl-TROSY NMR

Mitochondrial apoptotic signaling cascades lead to the formation of the apoptosome, a 1.1-MDa heptameric protein scaffold that recruits and activates the caspase-9 protease. Once activated, caspase-9 cleaves and activates downstream effector caspases, triggering the onset of cell death through caspase-mediated proteolysis of cellular proteins. Failure to activate caspase-9 enables the evasion of programmed cell death, which occurs in various forms of cancer. Despite the critical apoptotic...

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0 Replies | 189 Views
[NMR paper] 19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor
Dec 11, 2023 - 11:33 PM - by nmrlearner
nmrlearner's Avatar 19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor

For the A(2A) adenosine receptor (A(2A)AR), a class A G-protein-coupled receptor (GPCR), reconstituted in n-dodecyl-?-D-maltoside (DDM)/?????cholesteryl hemisuccinate (CHS) mixed micelles, previous ^(19)F-NMR studies revealed the presence of multiple simultaneously populated conformational states. Here, we study the influence of a different detergent, lauryl maltose neopentyl glycol (LMNG) in mixed micelles with CHS, and of lipid bilayer nanodiscs on these conformational equilibria. The...

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0 Replies | 122 Views
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