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Insights Into the Structure and Function of the “Immortality Protein” - Technology Networks
Sep 18, 2020 - 7:30 AM - by nmrlearner
nmrlearner's Avatar Insights Into the Structure and Function of the “Immortality Protein” - Technology Networks

Insights Into the Structure and Function of the “Immortality Protein” Technology Networks Read here
0 Replies | 7 Views
Analysis of {beta}2AR-Gs and {beta}2AR-Gi complex formation by NMR spectroscopy [Pharmacology]
Sep 17, 2020 - 7:46 AM - by nmrlearner
nmrlearner's Avatar Analysis of {beta}2AR-Gs and {beta}2AR-Gi complex formation by NMR spectroscopy [Pharmacology]

Xiuyan Ma, Yunfei Hu, Hossein Batebi, Jie Heng, Jun Xu, Xiangyu Liu, Xiaogang Niu, Hongwei Li, Peter W. Hildebrand, Changwen Jin, Brian K. Kobilka...
Date: 2020-09-15

The ?2-adrenergic receptor (?2AR) is a prototypical G protein-coupled receptor (GPCR) that preferentially couples to the stimulatory G protein Gs and stimulates cAMP formation. Functional studies have shown that the ?2AR also couples to inhibitory G protein Gi, activation of which inhibits cAMP formation [R. P. Xiao, Sci. STKE 2001,... Read More


PNAS:
Number: 37
Volume: 117
0 Replies | 18 Views
[NMR paper] Characterization of LP-Z Lipoprotein Particles and Quantification in Subjects with Liver Disease Using a Newly Developed NMR-Based Assay.
Sep 17, 2020 - 7:46 AM - by nmrlearner
nmrlearner's Avatar Characterization of LP-Z Lipoprotein Particles and Quantification in Subjects with Liver Disease Using a Newly Developed NMR-Based Assay.

Related Articles Characterization of LP-Z Lipoprotein Particles and Quantification in Subjects with Liver Disease Using a Newly Developed NMR-Based Assay.

J Clin Med. 2020 Sep 10;9(9):

Authors: Bedi S, Garcia E, Jeyarajah EJ, Shalaurova I, Perez-Matos MC, Jiang ZG, Dullaart RPF, Matyus SP, Kirk WJ, Otvos JD, Davidson WS, Connelly MA

Abstract
BACKGROUND: Lipoprotein particles with abnormal compositions, such as lipoprotein X (LP-X) and lipoprotein Z (LP-Z), have been described in cases of obstructive jaundice and cholestasis. The study objectives were to: (1) develop an NMR-based assay for quantification of plasma/serum LP-Z particles, (2) evaluate the assay performance, (3) isolate LP-Z particles and characterize them by lipidomic and proteomic analysis, and (4) quantify LP-Z in subjects with various liver diseases.
METHODS: Assay performance was assessed for linearity, sensitivity, and precision. Mass spectroscopy was used to characterize the protein and lipid content of isolated LP-Z particles.
RESULTS: The assay showed good linearity and precision (2.5-6.3%). Lipid analyses revealed that LP-Z particles exhibit lower cholesteryl esters and higher free cholesterol, bile acids, acylcarnitines, diacylglycerides,... [Read More]
0 Replies | 14 Views
[NMR paper] CHESPA/CHESCA-SPARKY: automated NMR data analysis plugins for SPARKY to map protein allostery.
Sep 17, 2020 - 7:46 AM - by nmrlearner
nmrlearner's Avatar CHESPA/CHESCA-SPARKY: automated NMR data analysis plugins for SPARKY to map protein allostery.

Related Articles CHESPA/CHESCA-SPARKY: automated NMR data analysis plugins for SPARKY to map protein allostery.

Bioinformatics. 2020 Sep 14;:

Authors: Shao H, Boulton S, Olivieri C, Mohamed H, Akimoto M, Subrahmanian MV, Veglia G, Markley JL, Melacini G, Lee W

Abstract
MOTIVATION: Correlated NMR chemical shift changes identified through the CHEmical Shift Projection Analysis (CHESPA) and CHEmical Shift Covariance Analysis (CHESCA) reveal pathways of allosteric transitions in biological macromolecules. To address the need for an automated platform that implements CHESPA and CHESCA and integrates them with other NMR analysis software packages, we introduce here integrated plugins for NMRFAM-SPARKY that implement the seamless detection and visualization of allosteric networks.
AVAILABILITY: CHESCA-SPARKY and CHESPA-SPARKY are available in the latest version of NMRFAM-SPARKY from the National Magnetic Resonance... [Read More]
0 Replies | 14 Views
[NMR paper] Packing Structure of Antiparallel ?-Sheet Polyalanine Region in a Sequential Model Peptide of Nephila clavipes Dragline Silk Studied Using 13C Solid-State NMR and MD Simulation.
Sep 17, 2020 - 7:46 AM - by nmrlearner
nmrlearner's Avatar Packing Structure of Antiparallel ?-Sheet Polyalanine Region in a Sequential Model Peptide of Nephila clavipes Dragline Silk Studied Using 13C Solid-State NMR and MD Simulation.

Related Articles Packing Structure of Antiparallel ?-Sheet Polyalanine Region in a Sequential Model Peptide of Nephila clavipes Dragline Silk Studied Using 13C Solid-State NMR and MD Simulation.

Biomacromolecules. 2019 10 14;20(10):3884-3894

Authors: Asakura T, Nishimura A, Aoki A, Naito A

Abstract
Packing structures of polyalanine regions, which are considered to be the reason for the extremely high strength of spider dragline silks, were studied using a series of sequential peptides: (Glu)4GlyGlyLeuGlyGlyGlnGlyAlaGly(Ala)nGlyGlyAlaGl yGlnGlyGlyTyrGlyGly(Glu)4 (n = 3-8) using 13C solid-state NMR spectroscopy. The conformations of (Ala)n in the freeze-dried peptides changed gradually with increasing n from random coils to ?-helices with partial antiparallel ?-sheet (AP-?) structures. Conversely, all the insolubilized peptides, n = 6-8 after low-pH treatment and n = 4-8 after formic... [Read More]
0 Replies | 12 Views
[NMR paper] NMR structures and mutational analysis of the two peptides constituting the bacteriocin plantaricin S.
Sep 17, 2020 - 7:46 AM - by nmrlearner
nmrlearner's Avatar NMR structures and mutational analysis of the two peptides constituting the bacteriocin plantaricin S.

Related Articles NMR structures and mutational analysis of the two peptides constituting the bacteriocin plantaricin S.

Sci Rep. 2019 02 20;9(1):2333

Authors: Ekblad B, Kristiansen PE

Abstract
The structure of the individual peptides of the two-peptide bacteriocin plantaricin S, an antimicrobial peptide produced by a Lactobacillus plantarum strain, has been determined in DPC micelles. The two peptides of plantaricin S, Pls-? and Pls-?, form an ?-helix from and including residue 8 to 24 with a less structured region around residue 16-19 and an amphiphilic ?-helix from and including residue 7 to 23, respectively.... [Read More]
0 Replies | 15 Views
[NMR paper] Sex differences in the 1H NMR metabolic profile of serum in cardiovascular risk patients.
Sep 17, 2020 - 7:46 AM - by nmrlearner
nmrlearner's Avatar Sex differences in the 1H NMR metabolic profile of serum in cardiovascular risk patients.

Related Articles Sex differences in the 1H NMR metabolic profile of serum in cardiovascular risk patients.

Sci Rep. 2019 02 20;9(1):2380

Authors: Barba I, Andrés M, Picón I, Aguade-Bruix S, Garcia-Dorado D

Abstract
Personalized diagnosis and risk stratification of cardiovascular diseases would allow optimizing therapeutic strategies and lifestyle changes. Metabolomics is a promising technique for personalized diagnosis and prognosis; however, various physiological parameters, including sex, influence the metabolic profile thus hampering its translation to the clinic. Knowledge of the variation in the metabolic profile... [Read More]
0 Replies | 17 Views
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