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[NMR paper] Protein Allostery Study in Cells Using NMR Spectroscopy
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar Protein Allostery Study in Cells Using NMR Spectroscopy

Protein allostery is commonly observed in vitro. But how protein allostery behaves in cells is unknown. In this work, a protein monomer-dimer equilibrium system was built with the allosteric effect on the binding characterized using NMR spectroscopy through mutations away from the dimer interface. A chemical shift linear fitting method was developed that enabled us to accurately determine the dissociation constant. A total of 28 allosteric mutations were prepared and grouped to negative...

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[NMR paper] Imaging of adeno-associated viral capsids for purposes of gene editing using CEST NMR/MRI
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar Imaging of adeno-associated viral capsids for purposes of gene editing using CEST NMR/MRI

CONCLUSION: AAV2 viral capsids exhibit strong capacity as an endogenous CEST contrast agent and can potentially be used for monitoring and evaluation of AAV vector-mediated gene therapy protocols.

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0 Replies | 30 Views
[NMR paper] Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure

Domain Z7 of nuclear transcription factor ZNF711 has the consensus last metal-ligand H23 found in odd-numbered zinc-fingers of this protein replaced by a phenylalanine. Ever since the discovery of ZNF711 it has been thought that Z7 is probably non-functional because of the H23F substitution. The presence of H26 three positions downstream prompted us to examine if this histidine could substitute as the last metal ligand. The Z7 domain adopts a stable tertiary structure upon metal binding. The NMR...

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0 Replies | 23 Views
Optimal isotope labelling for NMR protein structure determinations - Nature.com
Apr 22, 2024 - 2:18 PM - by nmrlearner
nmrlearner's Avatar Optimal isotope labelling for NMR protein structure determinations - Nature.com

Optimal isotope labelling for NMR protein structure determinations Nature.com Read here
0 Replies | 49 Views
Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... - Yahoo Finance
Apr 21, 2024 - 7:50 AM - by nmrlearner
nmrlearner's Avatar Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... - Yahoo Finance

Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... Yahoo Finance Read here
0 Replies | 54 Views
Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... - Financial Times
Apr 21, 2024 - 7:50 AM - by nmrlearner
nmrlearner's Avatar Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... - Financial Times

Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane Proteins and ... Financial Times Read here
0 Replies | 55 Views
[NMR paper] Quantification of Biopharmaceutically Relevant Nonionic Surfactant Excipients Using Benchtop qNMR
Apr 18, 2024 - 7:01 PM - by nmrlearner
nmrlearner's Avatar Quantification of Biopharmaceutically Relevant Nonionic Surfactant Excipients Using Benchtop qNMR

Nonionic surfactant excipients (NISEs) are commonly added to biologics formulations to mitigate the effects of stress incurred by the active biotherapeutic during manufacturing, transport, and storage. During manufacturing, NISEs are added by dilution of a stock solution directly into a protein formulation, and their accurate addition is critical in maintaining the quality and integrity of the drug product and thus ensuring patient safety. This is especially true for the common NISEs,...

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