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 How wide is the window opened by high-resolution relaxometry on the internal dynamics of proteins in solution? |
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Mar 23, 2021 - 7:56 PM - by nmrlearner
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How wide is the window opened by high-resolution relaxometry on the internal dynamics of proteins in solution?
Abstract
The dynamics of molecules in solution is usually quantified by the determination of timescale-specific amplitudes of motions. High-resolution nuclear magnetic resonance (NMR) relaxometry experimentsâ??where the sample is transferred to low fields for longitudinal (T1) relaxation, and back to high field for detection with residue-specific resolutionâ??seeks to increase the ability to distinguish the contributions from motion on timescales slower than a few nanoseconds. However, tumbling of a molecule in solution masks some of these motions. Therefore, we investigate to what extent relaxometry improves timescale resolution, using the â??detectorâ?? analysis of dynamics. Here, we demonstrate improvements in the characterization of internal dynamics of methyl-bearing side chains by carbon-13 relaxometry in the small protein ubiquitin. We show that relaxometry data leads to better information about nanosecond motions as compared to high-field relaxation data only. Our calculations show that gains from relaxometry are greater with increasing correlation time of rotational diffusion.
Source: Journal of Biomolecular NMR
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0 Replies | 30 Views
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Protein Crystallization & Crystallography Market Upcoming Challenges, Opportunities and Forecast, Global Size, Status and Forecast, Market Strategy, Outlook and Product Development, Market Segmentation By And Region, Type and Application - The Bi |
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Mar 23, 2021 - 7:56 PM - by nmrlearner
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Protein Crystallization & Crystallography Market Upcoming Challenges, Opportunities and Forecast, Global Size, Status and Forecast, Market Strategy, Outlook and Product Development, Market Segmentation By And Region, Type and Application - The Bisouv Network
Protein Crystallization & Crystallography Market Upcoming Challenges, Opportunities and Forecast, Global Size, Status and Forecast, Market Strategy, Outlook and Product Development, Market Segmentation By And Region, Type and Application The Bisouv Network Read here
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0 Replies | 16 Views
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