BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 




Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


» Online Users: 62
0 members and 62 guests
No Members online
Most users ever online was 292, 07-25-2011 at 03:18 PM.
» Welcome!

Welcome, NMR world!


Our visitors map.


» Log in
User Name Not a member yet?
Register Now!
Password
DELTEX2 C-terminal domain recognizes and recruits ADP-ribosylated proteins for ubiquitination - Science Advances
Aug 26, 2020 - 2:46 PM - by nmrlearner
nmrlearner's Avatar DELTEX2 C-terminal domain recognizes and recruits ADP-ribosylated proteins for ubiquitination Science Advances
DELTEX2 C-terminal domain recognizes and recruits ADP-ribosylated proteins for ubiquitination - Science Advances
More...
0 Replies | 12 Views
Protein Crystallization Market 2020: Potential growth, attractive valuation make it is a long-term investment | Know the COVID19 Impact | Top Players: Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher, etc. | InForGrowth - T
Aug 26, 2020 - 2:46 PM - by nmrlearner
nmrlearner's Avatar Protein Crystallization Market 2020: Potential growth, attractive valuation make it is a long-term investment | Know the COVID19 Impact | Top Players: Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher, etc. | InForGrowth - The Scarlet

Protein Crystallization Market 2020: Potential growth, attractive valuation make it is a long-term investment | Know the COVID19 Impact | Top Players: Hampton Research, Molecular Dimensions, PerkinElmer, GE Healthcare, Danaher, etc. | InForGrowth The Scarlet Read here
0 Replies | 18 Views
[NMR paper] Conformational ensembles of an intrinsically disordered protein consistent with NMR, SAXS and single-molecule FRET.
Aug 26, 2020 - 2:46 PM - by nmrlearner
nmrlearner's Avatar Conformational ensembles of an intrinsically disordered protein consistent with NMR, SAXS and single-molecule FRET.

Related Articles Conformational ensembles of an intrinsically disordered protein consistent with NMR, SAXS and single-molecule FRET.

J Am Chem Soc. 2020 Aug 25;:

Authors: Gomes GW, Krzeminski M, Namini A, Martin EW, Mittag T, Head-Gordon T, Forman-Kay JD, Gradinaru CC

Abstract
Intrinsically disordered proteins (IDPs) have fluctuating heterogeneous conformations, which makes structural characterization challenging, but of great interest, since their conformational ensembles are the link between their sequences and functions. An accurate description of IDP conformational ensembles depends crucially on the amount and quality of the experimental data, how it is integrated, and if it mutually supports a consistent structural picture. We used integrative modelling and validation to apply conformational restraints and assess agreement with the most common structural techniques for IDPs: Nuclear Magnetic Resonance (NMR) spectroscopy, Small-angle X-ray Scattering (SAXS), and single-molecule Förster Resonance Energy Transfer (smFRET) reach concordance on structural ensembles for Sic1 and phosphorylated Sic1 (pSic1). To resolve apparent discrepancies between smFRET and SAXS, we integrated SAXS data with non-smFRET (NMR) data and reserved the new smFRET data for Sic1 and pSic1, as an... [Read More]
0 Replies | 13 Views
Global Protein Trends & Technologies Market : Industry Analysis and Forecast (2020-2027) - Good Night, Good Hockey
Aug 25, 2020 - 1:19 PM - by nmrlearner
nmrlearner's Avatar Global Protein Trends & Technologies Market : Industry Analysis and Forecast (2020-2027) Good Night, Good Hockey
Global Protein Trends & Technologies Market : Industry Analysis and Forecast (2020-2027) - Good Night, Good Hockey
More...
0 Replies | 13 Views
[NMR paper] Structural characterization of a dimeric complex between the short cytoplasmic domain of CEACAM1 and the pseudo tetramer of S100A10-Annexin A2 using NMR and molecular dynamics.
Aug 25, 2020 - 1:19 PM - by nmrlearner
nmrlearner's Avatar Structural characterization of a dimeric complex between the short cytoplasmic domain of CEACAM1 and the pseudo tetramer of S100A10-Annexin A2 using NMR and molecular dynamics.

Related Articles Structural characterization of a dimeric complex between the short cytoplasmic domain of CEACAM1 and the pseudo tetramer of S100A10-Annexin A2 using NMR and molecular dynamics.

Biochim Biophys Acta Biomembr. 2020 Aug 21;:183451

Authors: Hu W, Bhattacharya S, Hong T, Wong P, Li L, Vaidehi N, Kalkum M, Shively JE

Abstract
AIIt, a heterotetramer of S100A10 (P11) and Annexin A2, plays a key role in calcium dependent, membrane associations with a variety of proteins. We previously showed that AIIt interacts with the short cytoplasmic domain (12 amino acids) of CEACAM1 (CEACAM1-SF). Since the cytoplasmic domains of CEACAM1 help regulate the formation of cis- or trans-dimers at the cell membrane, we investigated the possible role of their association with AIIt in this process. Using NMR and molecular dynamics, we show that AIIt and its pseudoheterodimer interacts with two molecules of short cytoplasmic domain isoform peptides, and that interaction depends on the binding motif 454-Phe-Gly-Lys-Thr-457 where Phe-454 binds in a hydrophobic pocket of AIIt, the null mutation Phe454Ala reduces binding by 2.5 fold, and the pseudophosphorylation mutant Thr457Glu reduces binding by three fold. Since these two... [Read More]
0 Replies | 25 Views
[NMR paper] Solution structure of insect CSP and OBPs by NMR.
Aug 24, 2020 - 9:54 PM - by nmrlearner
nmrlearner's Avatar Solution structure of insect CSP and OBPs by NMR.

Solution structure of insect CSP and OBPs by NMR.

Methods Enzymol. 2020;642:169-192

Authors: Leone S, Emendato A, Spadaccini R, Picone D

Abstract
Insect odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are proteins deputed to the solubilization, transport and stabilization of lipophilic and odorant compounds. These proteins have a conserved fold, which undergoes massive structural rearrangements in order to accommodate medium to large-sized lipophilic ligands. Solution NMR spectroscopy, due to its intrinsically dynamic nature, is the perfect technique to extrapolate structural information and dynamic parameters and to elucidate the conformational changes that occur upon ligand binding. This chapter will describe in detail the experimental protocols for the production and purification of isotope-labeled recombinant CSPs and OBPs for NMR studies. Detailed procedures for spectra acquisition, processing and analysis will be presented, focusing on the protein CSP-sg4 from Schistocerca gregaria as a model. Finally, experiments aimed at providing information on protein flexibility and ligand binding modes will also be described.


PMID: 32828252 [PubMed - as supplied by publisher]



More...
0 Replies | 25 Views
Catalytic cycle of carbohydrate dehydration by Lewis acids: structures and rates from synergism of conventional and DNP NMR #DNPNMR #DDNP
Aug 24, 2020 - 9:54 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Catalytic cycle of carbohydrate dehydration by Lewis acids: structures and rates from synergism of conventional and DNP NMR #DNPNMR #DDNP


Jensen, Pernille Rose, and Sebastian Meier. “Catalytic Cycle of Carbohydrate Dehydration by Lewis Acids: Structures and Rates from Synergism of Conventional and DNP NMR.” Chemical Communications 56, no. 46 (June 9, 2020): 6245–48.


https://doi.org/10.1039/D0CC01756F.


Lewis acids play key roles in many chemical reactions. Structural and functional (kinetic) detail in Lewis acid catalysed fructose conversion are derived herein by the combined use of conventional and dissolution dynamic nuclear polarization (D-DNP) NMR. Structural information obtained with D-DNP NMR was used to identify conditions that stabilize an elusive initial intermediate and to determine its chemical structure. Carbohydrate dehydration through this intermediate had been predicted computationally. Complementary kinetic NMR assays yielded rate constants spanning three orders of magnitude for the three biggest energy barriers in the catalytic cycle.


Go to The DNP-NMR Blog for more info.
0 Replies | 26 Views
» Stats
Members: 3,202
Threads: 22,731
Posts: 23,117
Top Poster: nmrlearner (20,175)
Welcome to our newest member, bpadmanabhan
Powered by vBadvanced CMPS v3.2.2

All times are GMT. The time now is 05:36 AM.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2020, Jelsoft Enterprises Ltd.
vBCredits II Deluxe v1.2.0 Copyright © 2010 DragonByte Technologies
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

Map