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Global Protein Trends & Technologies Market 2020 Top Key Players | Rigaku Corporation (Japan), Hampton Research Corp. (U.S.), Jena Bioscience GmbH (Germany), Thermo Fisher Scientific Inc. (U.S), Molecular Dimensions Ltd. (U.K.) | Impact of COVID-
May 21, 2020 - 8:29 PM - by nmrlearner
nmrlearner's Avatar Global Protein Trends & Technologies Market 2020 Top Key Players | Rigaku Corporation (Japan), Hampton Research Corp. (U.S.), Jena Bioscience GmbH (Germany), Thermo Fisher Scientific Inc. (U.S), Molecular Dimensions Ltd. (U.K.) | Impact of COVID-19 Galus Australis
Global Protein Trends & Technologies Market 2020 Top Key Players | Rigaku Corporation (Japan), Hampton Research Corp. (U.S.), Jena Bioscience GmbH (Germany), Thermo Fisher Scientific Inc. (U.S), Molecular Dimensions Ltd. (U.K.) | Impact of COVID-19 - Galus Australis
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0 Replies | 51 Views
Postdoc in Protein Dynamics & NMR Spectroscopy - Nature.com
May 21, 2020 - 8:29 PM - by nmrlearner
nmrlearner's Avatar Postdoc in Protein Dynamics & NMR Spectroscopy - Nature.com

Postdoc in Protein Dynamics & NMR Spectroscopy Nature.com Read here
0 Replies | 51 Views
[NMR paper] The near-symmetry of protein oligomers: NMR-derived structures.
May 21, 2020 - 8:29 PM - by nmrlearner
nmrlearner's Avatar The near-symmetry of protein oligomers: NMR-derived structures.

The near-symmetry of protein oligomers: NMR-derived structures.

Sci Rep. 2020 May 20;10(1):8367

Authors: Bonjack M, Avnir D

Abstract
The majority of oligomeric proteins form clusters which have rotational or dihedral symmetry. Despite the many advantages of symmetric packing, protein oligomers are only nearly symmetric, and the origin of this phenomenon is still in need to be fully explored. Here we apply near-symmetry analyses by the Continuous Symmetry Measures methodology of protein homomers to their natural state, namely their structures in solution. NMR-derived structural data serves us for that purpose. We find that symmetry deviations of proteins are by far higher in solution, compared to the crystalline state; that much of the symmetry distortion is due to amino acids along the interface between the subunits; that the distortions are mainly due to hydrophilic amino acids; and that distortive oligomerization processes such as the swap-domain mechanism can be identified by the symmetry analysis. Most of the analyses were carried out on distorted C2-symmetry dimers, but C3 and D2 cases were analyzed as well. Our NMR analysis supports the idea that the crystallographic B-factor represents non-classical crystals, in which different conformers pack in the crystal, perhaps from the conformers which the NMR analysis provides.


PMID: 32433550 [PubMed - as supplied by publisher]



... [Read More]
0 Replies | 54 Views
[NMR paper] Precision Conjugation: An Emerging Tool for Generating Protein-Polymer Conjugates
May 21, 2020 - 8:29 PM - by nmrlearner
nmrlearner's Avatar Precision Conjugation: An Emerging Tool for Generating Protein-Polymer Conjugates


Angewandte Chemie International Edition, Accepted Article.

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0 Replies | 56 Views
[NMR paper] Monitoring protein ubiquitination and SUMOylation in real-time by NMR.
May 20, 2020 - 5:17 PM - by nmrlearner
nmrlearner's Avatar Monitoring protein ubiquitination and SUMOylation in real-time by NMR.

Monitoring protein ubiquitination and SUMOylation in real-time by NMR.

Chem Commun (Camb). 2020 May 19;:

Authors: Habibullah BI, Tripathi V, Surana P, Das R

Abstract
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.


PMID: 32426760 [PubMed - as supplied by publisher]



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0 Replies | 63 Views
Relaxation Mechanisms #DNPNMR #EPR
May 20, 2020 - 5:17 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

Relaxation Mechanisms #DNPNMR #EPR

This is an excellent review and summary on different relaxation mechanisms observed in EPR spectroscopy. Understanding EPR relaxation is crucial to understand the DNP process.




Eaton, Sandra S., and Gareth R. Eaton. “Relaxation Mechanisms.” In EMagRes, edited by Robin K. Harris and Roderick L. Wasylishen, 1543–56. Chichester, UK: John Wiley & Sons, Ltd, 2016.


https://doi.org/10.1002/9780470034590.emrstm1507.


After a paramagnetic species absorbs energy, there are various relaxation processes by which the excitation energy is lost to the surroundings thereby enabling return to the ground state. The focus of this article is on relaxation of species with S= 1/2 in magnetically dilute samples. The relative importance of various spin–lattice relaxation processes for each paramagnetic species is strongly dependent on temperature, electronic, and molecular structure. The Raman and local-mode processes make significant contributions to T 1 relaxation in rigid and semirigid lattices for a wide range of species at temperature above about 10 K. The Orbach process requires a low-lying excited state. The thermally activated process is significant when a stochastic process averages inequivalent environments on a timescale comparable to the Larmor frequency, as occurs by rotation of methyl groups or hopping of a hydrogen-bonded proton. Spin-echo dephasing at low temperatures is dominated by nuclear spin diffusion. It is... [Read More]
0 Replies | 51 Views
[NMR paper] Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.
May 19, 2020 - 9:51 PM - by nmrlearner
nmrlearner's Avatar Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.

Related Articles Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.

Langmuir. 2020 May 16;:

Authors: Meikle T, Keizer DW, Babon JJ, Drummond CJ, Separovic F, Conn CE, Yao S

Abstract
Lipidic inverse bicontinuous cubic phases (LCPs), formed via the spontaneous self-assembly of lipids such as monoolein, have found increasing applications in the stabilization and crystallization of integral membrane proteins for structural characterization using X-ray crystallography. Their use as effective drug release matrices has also been demonstrated. Nuclear magnetic resonance (NMR) spectroscopy, both solution and solid-state, has previously been employed for the characterization of LCPs and related systems. Herein, we report a number of novel features of solution NMR for probing the fundamental composition and structural properties of monoolein-based LCPs. These include: (1) more complete assignments of both 1H and 13C chemical shifts, (2) direct quantification of hydration level in LCPs using 1D 1H NMR, and (3) monitoring longer term stability of LCPs and evaluating alterations introduced into standard LCPs at the sub-molecular level.


PMID: 32418433 [PubMed - as supplied by publisher]



... [Read More]
0 Replies | 49 Views
[NMR] Continuing Search for Research Faculty – 36T SCH Solid-State NMR at NHMFL
May 19, 2020 - 9:51 PM - by nmrlearner
nmrlearner's Avatar From The DNP-NMR Blog:

[NMR] Continuing Search for Research Faculty – 36T SCH Solid-State NMR at NHMFL

Research Faculty – 36T SCH Solid-State NMR


The National High Magnetic Field Laboratory (NHMFL) of the U.S. has an opening for a solid-state NMR Research Faculty position. The primary responsibilities will be developing a research and collaborative user program utilizing the 36 Tesla Series-Connected-Hybrid (SCH), a 12 MWatt powered magnet supporting sub-ppm NMR at 1.5 GHz. Equipped with a custom-made Bruker NEO console, a unique field regulation system and a suite of MAS and oriented sample solid-state NMR probes developed at the NHMFL the 36T SCH magnet provides the highest magnetic field available today capable for high-resolution solid-state NMR of biosolids and materials applications [J. Magn. Reson., 284, 125-136 (2017)]. The Research Faculty will work with a team of engineers and scientists and be responsible for: (1) developing a research program in biosolids and in materials applications with quadrupolar and spin-1/2 nuclei utilizing the high-field magnets at the NHMFL; (2) supporting the 36T SCH user program, that includes NMR system management, project initiation, planning and execution with users. This Faculty is expected to be a motivated researcher with excellent communication skills, a collegial personality, a team-player, and a scientist with the vision to become a world-class researcher.


The NHMFL’s NMR/MRI user program operates a state-of-the-art user facility at the Magnet Lab conducting research at the frontiers of physics, chemistry, biology, material sciences with unique magnet technologies. It has an outstanding infrastructure with an array of NMR... [Read More]
0 Replies | 49 Views
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