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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password F NMR spectroscopy Mar 24, 2025 - 3:53 PM - by nmrlearner F NMR spectroscopy The cellular environment is a complex and crowded space, with organelles, compartments and multitudes of molecules engaged in intricate networks of communication that modulate binary protein-ligand/protein interactions. As a result, it is becoming increasingly appreciated that evaluations of protein-drug binding should be carried out in the native cellular environment. Here, we present a proof-of-concept study where we measured the lifetime (1/koff) of a protein-drug complex in human cells by 19F NMR spectroscopy using fluorinated Cyclophilin A (CypA) bound to Cyclosporine A (CsA). Harnessing the exceptional detection sensitivity of the trifluoromethyl group attached at the para position of Phe60 in CypA, high-quality 2D 19F-19F exchange spectra were obtained in cells. Essentially identical koff values were observed in cells and in vitro, suggesting that the overall impact of the cellular environment on the lifetime of tfmF60 CypA/CsA complex is minimal. Using similar approaches for quantifying protein-drug lifetimes in the native cellular environment paves the way for efficiently screening drug libraries in human cells by 19F NMR spectroscopy. Source: Journal of Biomolecular NMR 0 Replies | 46 Views Genetic Encoding of Fluoro-l-tryptophans for Site-Specific Detection of Conformational Heterogeneity in Proteins by NMR Spectroscopy - ACS Publications Mar 24, 2025 - 3:53 PM - by nmrlearner Genetic Encoding of Fluoro-l-tryptophans for Site-Specific Detection of Conformational Heterogeneity in Proteins by NMR Spectroscopy - ACS Publications Genetic Encoding of Fluoro-l-tryptophans for Site-Specific Detection of Conformational Heterogeneity in Proteins by NMR Spectroscopy ACS Publications Read here 0 Replies | 30 Views F NMR-Monitored Chemical Denaturation of a Fold-Switching Protein RfaH Reveals Its Slow Folding Dynamics - ACS Publications Mar 24, 2025 - 3:53 PM - by nmrlearner F NMR-Monitored Chemical Denaturation of a Fold-Switching Protein RfaH Reveals Its Slow Folding Dynamics - ACS Publications F NMR-Monitored Chemical Denaturation of a Fold-Switching Protein RfaH Reveals Its Slow Folding Dynamics ACS Publications Read here 0 Replies | 20 Views Protein recognition by NMR - Nature.com Mar 24, 2025 - 3:53 PM - by nmrlearner Protein recognition by NMR - Nature.com Protein recognition by NMR Nature.com Read here 0 Replies | 17 Views Quantitating Metabolites in Protein Precipitated Serum Using NMR Spectroscopy - ACS Publications Mar 24, 2025 - 3:53 PM - by nmrlearner Quantitating Metabolites in Protein Precipitated Serum Using NMR Spectroscopy - ACS Publications Quantitating Metabolites in Protein Precipitated Serum Using NMR Spectroscopy ACS Publications Read here 0 Replies | 31 Views [NMR paper] Refolding of the Deinococcus Radiodurans phytochrome photosensory module and an extended backbone resonance assignment by solution NMR Mar 24, 2025 - 3:53 PM - by nmrlearner Refolding of the Deinococcus Radiodurans phytochrome photosensory module and an extended backbone resonance assignment by solution NMR Solution NMR reveals the structure and dynamics of biomolecules in solution. In particular, the method can detect changes due to perturbation of the molecules, without limiting effects of frozen particles or crystal environments. Phytochromes are photosensors which control the response to red/far-red light in bacteria, fungi and plants, undergo specific structural changes when photoactivated from the Pr to the Pfr state. While structures of phytochromes have been revealed in both states, the... More... 0 Replies | 37 Views [NMR paper] Solid-state NMR observation of chitin in whole cells by indirect (15)N detection with NC, NCC, CNC and CNCC polarization transfers Mar 23, 2025 - 9:41 PM - by nmrlearner Solid-state NMR observation of chitin in whole cells by indirect (15)N detection with NC, NCC, CNC and CNCC polarization transfers Chitin is the most important nitrogen-containing polysaccharide found on Earth. This polysaccharide is a polymer of an N-acetylglucosamine and it is a crucial structural component of fungal cell walls and crustaceans. Magic-angle spinning solid-state NMR is emerging as a powerful analytical approach to study polysaccharides in the context of intact cell walls and whole cells. The presence of an acetamido group in chitin is attractive for ^(15)N solid-state NMR. Here we investigate the use of... More... 0 Replies | 61 Views [NMR paper] In situ light-driven pH modulation for NMR studies Mar 23, 2025 - 9:41 PM - by nmrlearner In situ light-driven pH modulation for NMR studies Proton exchange is a fundamental chemical event, and NMR provides the most direct readout of protonation events with site-specific resolution. Conventional approaches require manual titration of sample pH to collect a series of NMR spectra at different pH values. This requires extensive sample handling and often results in significant sample loss, leading to reduced signal or the need to prepare additional samples. Here, we introduce a novel approach to control pH in NMR samples using water... 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