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Development of an In Situ G Protein-Coupled Receptor Fragment Molecule Screening Approach with High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance - ACS Publications
Feb 04, 2025 - 10:40 AM - by nmrlearner
nmrlearner's Avatar Development of an In Situ G Protein-Coupled Receptor Fragment Molecule Screening Approach with High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance ACS Publications
Development of an In Situ G Protein-Coupled Receptor Fragment Molecule Screening Approach with High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance - ACS Publications
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0 Replies | 43 Views
Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR - ACS Publications
Feb 03, 2025 - 6:10 PM - by nmrlearner
nmrlearner's Avatar Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR - ACS Publications

Assessing Site-Specific Enhancements Imparted by Hyperpolarized Water in Folded and Unfolded Proteins by 2D HMQC NMR ACS Publications Read here
0 Replies | 45 Views
[NMR paper] In situ NMR reveals a pH sensor motif in an outer membrane protein that drives bacterial vesicle production
Feb 03, 2025 - 6:10 PM - by nmrlearner
nmrlearner's Avatar In situ NMR reveals a pH sensor motif in an outer membrane protein that drives bacterial vesicle production

The outer membrane vesicles (OMVs) produced by diderm bacteria have important roles in cell envelope homeostasis, secretion, interbacterial communication, and pathogenesis. The facultative intracellular pathogen Salmonella enterica Typhimurium (STm) activates OMV biogenesis inside the acidic vacuoles of host cells by upregulating the expression of the outer membrane (OM) protein PagC, one of the most robustly activated genes in a host environment. Here, we used solid-state nuclear magnetic...

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0 Replies | 125 Views
NMR Methods for Structural Characterization of Protein-Protein Complexes - Frontiers
Feb 03, 2025 - 6:00 AM - by nmrlearner
nmrlearner's Avatar NMR Methods for Structural Characterization of Protein-Protein Complexes - Frontiers

NMR Methods for Structural Characterization of Protein-Protein Complexes Frontiers Read here
0 Replies | 42 Views
NMR Experiments for Studies of Dilute and Condensed Protein Phases: Application to the Phase-Separating Protein CAPRIN1 - ACS Publications
Feb 01, 2025 - 10:06 PM - by nmrlearner
nmrlearner's Avatar NMR Experiments for Studies of Dilute and Condensed Protein Phases: Application to the Phase-Separating Protein CAPRIN1 - ACS Publications

NMR Experiments for Studies of Dilute and Condensed Protein Phases: Application to the Phase-Separating Protein CAPRIN1 ACS Publications Read here
0 Replies | 50 Views
NMR Structure Determination for Larger Proteins Using Backbone-Only Data - Science
Jan 30, 2025 - 3:23 PM - by nmrlearner
nmrlearner's Avatar NMR Structure Determination for Larger Proteins Using Backbone-Only Data - Science

NMR Structure Determination for Larger Proteins Using Backbone-Only Data Science Read here
0 Replies | 50 Views
[NMR paper] Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding
Jan 30, 2025 - 3:23 PM - by nmrlearner
nmrlearner's Avatar Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding

The NMR signals from protein sidechains are rich in information about intra- and inter-molecular interactions, but their detection can be complicated due to spectral overlap as well as conformational and hydrogen exchange. In this work, we demonstrate a protocol for multi-dimensional solid-state NMR spectral editing of signals from basic sidechains based on Hadamard matrix encoding. The Hadamard method acquires multi-dimensional experiments in such a way that both the backbone and under-sampled...

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0 Replies | 44 Views
[NMR paper] Time-resolved compositional and dynamics analysis of biofilm maturation and dispersal via solid-state NMR spectroscopy
Jan 30, 2025 - 3:23 PM - by nmrlearner
nmrlearner's Avatar Time-resolved compositional and dynamics analysis of biofilm maturation and dispersal via solid-state NMR spectroscopy

Dispersal plays a crucial role in the development and ecology of biofilms. While extensive studies focused on elucidating the molecular mechanisms governing this process, few have characterized the associated temporal changes in composition and structure. Here, we employed solid-state nuclear magnetic resonance (NMR) techniques to achieve time-resolved characterization of Bacillus subtilis biofilms over a 5-day period. The mature biofilm, established within 48 h, undergoes significant...

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0 Replies | 53 Views
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