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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password Protein folding and unfolding studied at atomic resolution by fast two-dimensional NMR spectroscopy - pnas.org Mar 08, 2025 - 1:15 AM - by nmrlearner Protein folding and unfolding studied at atomic resolution by fast two-dimensional NMR spectroscopy - pnas.org Protein folding and unfolding studied at atomic resolution by fast two-dimensional NMR spectroscopy pnas.org Read here 0 Replies | 36 Views [NMR paper] Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies Mar 07, 2025 - 4:49 AM - by nmrlearner Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies The use of Escherichia coli for recombinant protein production is a cornerstone in structural biology, particularly for nuclear magnetic resonance (NMR) spectroscopy studies. Understanding the metabolic behavior of E. coli under different carbon sources is critical for optimizing isotope labeling strategies, which are essential for protein structure determination by NMR. Recent advancements, such as mixed pyruvate labeling, have enabled improved backbone resonance assignment in large proteins,... More... 0 Replies | 180 Views Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies Mar 06, 2025 - 4:47 PM - by nmrlearner Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies The use of Escherichia coli for recombinant protein production is a cornerstone in structural biology, particularly for nuclear magnetic resonance (NMR) spectroscopy studies. Understanding the metabolic behavior of E. coli under different carbon sources is critical for optimizing isotope labeling strategies, which are essential for protein structure determination by NMR. Recent advancements, such as mixed pyruvate labeling, have enabled improved backbone resonance assignment in large proteins, making selective isotopic labeling strategies more important than ever for NMR studies. In this study, we aimed to investigate the metabolic adaptations of E. coli when grown on pyruvate as the sole carbon source, a common condition used to achieve selective labeling for NMR spectroscopy. Using NMR-based metabolomics, we tracked key metabolic shifts throughout the culture process to better understand how pyruvate metabolism affects protein production and isotopic labeling. Our results reveal that pyruvate is rapidly depleted before IPTG induction, while acetate and lactate accumulate due to overflow metabolism. These byproducts persist after induction, indicating that pyruvate is diverted into waste pathways, which limits its efficient use in isotope incorporation. This metabolic inefficiency presents a challenge for isotopic labeling protocols that rely on pyruvate as a carbon source for NMR studies. Our results highlight the need to fine-tune pyruvate supplementation to improve metabolic efficiency and isotopic labeling, making this study directly relevant to optimizing protocols for NMR studies involving protein structure determination. These insights provide valuable guidance for enhancing the quality and yield of isotopically labeled proteins in NMR... [Read More] 0 Replies | 72 Views Integrated description of protein dynamics from room-temperature X-ray crystallography and NMR - pnas.org Mar 06, 2025 - 4:47 PM - by nmrlearner Integrated description of protein dynamics from room-temperature X-ray crystallography and NMR - pnas.org Integrated description of protein dynamics from room-temperature X-ray crystallography and NMR pnas.org Read here 0 Replies | 36 Views Assessment of prediction methods for protein structures determined by NMR in CASP14: Impact of AlphaFold2 - Wiley Online Library Mar 06, 2025 - 4:47 PM - by nmrlearner Assessment of prediction methods for protein structures determined by NMR in CASP14: Impact of AlphaFold2 - Wiley Online Library Assessment of prediction methods for protein structures determined by NMR in CASP14: Impact of AlphaFold2 Wiley Online Library Read here 0 Replies | 41 Views Xplor-NIH: Better parameters and protocols for NMR protein structure determination - Wiley Online Library Mar 06, 2025 - 4:34 AM - by nmrlearner Xplor-NIH: Better parameters and protocols for NMR protein structure determination - Wiley Online Library Xplor-NIH: Better parameters and protocols for NMR protein structure determination Wiley Online Library Read here 0 Replies | 41 Views Protein NMR Structures Refined with Rosetta Have Higher Accuracy Relative to Corresponding X-ray Crystal Structures - ACS Publications Mar 05, 2025 - 2:05 PM - by nmrlearner Protein NMR Structures Refined with Rosetta Have Higher Accuracy Relative to Corresponding X-ray Crystal Structures - ACS Publications Protein NMR Structures Refined with Rosetta Have Higher Accuracy Relative to Corresponding X-ray Crystal Structures ACS Publications Read here 0 Replies | 44 Views [NMR paper] Hidden structural states of proteins revealed by conformer selection with AlphaFold-NMR Mar 04, 2025 - 11:03 PM - by nmrlearner Hidden structural states of proteins revealed by conformer selection with AlphaFold-NMR We introduce AlphaFold-NMR, a novel approach to NMR structure determination that reveals previously undetected protein conformational states. Unlike conventional NMR methods which rely on NOE-derived spatial restraints, AlphaFold-NMR combines AI-driven conformational sampling with Bayesian scoring of realistic protein models against NOESY and chemical shift data. This method uncovers alternative conformational states of the enzyme Gaussia luciferase, involving large-scale changes in the lid,... More... 0 Replies | 125 Views Page 11 of 100 « First < 9 10 11 12 13 21 61 > Last »

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