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[NMR paper] Protein Allostery Study in Cells Using NMR Spectroscopy |
Apr 24, 2024 - 5:58 AM - by nmrlearner
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Protein Allostery Study in Cells Using NMR Spectroscopy
Protein allostery is commonly observed in vitro. But how protein allostery behaves in cells is unknown. In this work, a protein monomer-dimer equilibrium system was built with the allosteric effect on the binding characterized using NMR spectroscopy through mutations away from the dimer interface. A chemical shift linear fitting method was developed that enabled us to accurately determine the dissociation constant. A total of 28 allosteric mutations were prepared and grouped to negative...
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