BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Home Forums Wiki NMR feeds Downloads Register Today's Posts



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


» Online Users: 314
0 members and 314 guests
No Members online
Most users ever online was 1,278, 01-09-2024 at 07:38 AM.
» Welcome!

Welcome, NMR world!


Our visitors map.


» Log in
User Name Not a member yet?
Register Now!
Password
Global fold and backbone dynamics of the hepatitis C virus E2 glycoprotein transmembrane domain determined by NMR - ScienceDirect.com
Apr 25, 2024 - 4:20 PM - by nmrlearner
nmrlearner's Avatar Global fold and backbone dynamics of the hepatitis C virus E2 glycoprotein transmembrane domain determined by NMR - ScienceDirect.com

Global fold and backbone dynamics of the hepatitis C virus E2 glycoprotein transmembrane domain determined by NMR ScienceDirect.com Read here
0 Replies | 1 Views
[NMR paper] NMR methods to detect fluoride binding and transport by membrane proteins
Apr 25, 2024 - 4:20 PM - by nmrlearner
nmrlearner's Avatar NMR methods to detect fluoride binding and transport by membrane proteins

Solid-state nuclear magnetic resonance (NMR) methods can probe the motions of membrane proteins in liposomes at the atomic level, and propel the understanding of biomolecular processes for which static structures cannot provide a satisfactory description. High-resolution crystallography snapshots have provided a structural basis for fluoride channels. NMR is a powerful tool to build upon these snapshots and depict a dynamic picture of fluoride channels in native-like lipid bilayers. In this...

More...
0 Replies | 2 Views
[NMR paper] Including the Ensemble of Unstructured Conformations in the Analysis of Protein's Native State by High-Pressure NMR Spectroscopy
Apr 25, 2024 - 4:15 AM - by nmrlearner
nmrlearner's Avatar Including the Ensemble of Unstructured Conformations in the Analysis of Protein's Native State by High-Pressure NMR Spectroscopy

The analysis of pressure induced changes in the chemical shift of proteins allows statements on structural fluctuations proteins exhibit at ambient pressure. The inherent issue of separating general pressure effects from structural related effects on the pressure dependence of chemical shifts has so far been addressed by considering the characteristics of random coil peptides on increasing pressure. In this work, chemically and pressure denatured states of the cold shock protein B from Bacillus...

More...
0 Replies | 30 Views
[NMR paper] Imaging of adeno-associated viral capsids for purposes of gene editing using CEST NMR/MRI
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar Imaging of adeno-associated viral capsids for purposes of gene editing using CEST NMR/MRI

CONCLUSION: AAV2 viral capsids exhibit strong capacity as an endogenous CEST contrast agent and can potentially be used for monitoring and evaluation of AAV vector-mediated gene therapy protocols.

More...
0 Replies | 14 Views
[NMR paper] Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar Formerly degenerate seventh zinc finger domain from transcription factor ZNF711 rehabilitated by experimental NMR structure

Domain Z7 of nuclear transcription factor ZNF711 has the consensus last metal-ligand H23 found in odd-numbered zinc-fingers of this protein replaced by a phenylalanine. Ever since the discovery of ZNF711 it has been thought that Z7 is probably non-functional because of the H23F substitution. The presence of H26 three positions downstream prompted us to examine if this histidine could substitute as the last metal ligand. The Z7 domain adopts a stable tertiary structure upon metal binding. The NMR...

More...
0 Replies | 11 Views
[NMR paper] Quantification of Residual Water in Pharmaceutical Frozen Solutions via (1)H Solid-state NMR
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar Quantification of Residual Water in Pharmaceutical Frozen Solutions via (1)H Solid-state NMR

Freezing is essential for the stability of biological drug substances and products, particularly in frozen solution formulations and during the primary drying of lyophilized preparations. However, the unfrozen segment within the frozen matrix can alter solute concentration, ionic strength, and stabilizer crystallization, posing risks of increased biophysical instability and faster chemical degradation. While quantifying the unfrozen water content is important for designing stable...

More...
0 Replies | 11 Views
[NMR paper] Solution NMR chemical shift assignment of apo and molybdate-bound ModA at two pHs
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar Solution NMR chemical shift assignment of apo and molybdate-bound ModA at two pHs

ModA is a soluble periplasmic molybdate-binding protein found in most gram-negative bacteria. It is part of the ABC transporter complex ModABC that moves molybdenum into the cytoplasm, to be used by enzymes that carry out various redox reactions. Since there is no clear analog for ModA in humans, this protein could be a good target for antibacterial drug design. Backbone ¹H, ^(13)C and ^(15)N chemical shifts of apo and molybdate-bound ModA from E. coli were assigned at pHs 6.0 and 4.5. In...

More...
0 Replies | 13 Views
[NMR paper] NMR metabolomic modeling of age and lifespan: A multicohort analysis
Apr 24, 2024 - 5:58 AM - by nmrlearner
nmrlearner's Avatar NMR metabolomic modeling of age and lifespan: A multicohort analysis

Metabolomic age models have been proposed for the study of biological aging, however, they have not been widely validated. We aimed to assess the performance of newly developed and existing nuclear magnetic resonance spectroscopy (NMR) metabolomic age models for prediction of chronological age (CA), mortality, and age-related disease. Ninety-eight metabolic variables were measured in blood from nine UK and Finnish cohort studies (N ?31,000 individuals, age range 24-86 years). We used nonlinear...

More...
0 Replies | 15 Views
» Stats
Members: 3,202
Threads: 25,743
Posts: 26,129
Top Poster: nmrlearner (23,187)
Welcome to our newest member, bpadmanabhan
Powered by vBadvanced CMPS v3.2.2

All times are GMT. The time now is 05:05 PM.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
vBCredits II Deluxe v1.2.0 Copyright © 2010 DragonByte Technologies
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

Map