Ask NMR questions! Earn NMR points Redeem NMR points Vote for NMR papers Twitter Ask to aggregate a site  Our Linkedin group BioNMR User Name Remember Me? Password Home Forums Wiki NMR feeds Downloads Register Today's Posts Search Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues NMR books: NMR books NMR theses: NMR theses All papers: All papers By topic: Protein-ligand interactions Solid-state high-resolution NMR NMR online resources: Educational web pages NMR presentations Educational web pages Educational videos News from other NMR forums NMR news NMR tweets NMR bookmarks NMR pictures NMR links BioNMR stuff: Feedback, suggestions, requests Announcements Test forum Sample preparation: Proteins General: General Software feature requests NMR software Spectrum analysis Molecular Modeling: NMR structure calculation Dynamics by NMR Structure analysis Molecular Dynamics Downloads: BioNMR downloads NMR processing: NMRPipe MDD NMR assignment: Backbone: Autoassign MARS UNIO Match PINE Side-chains: UNIO ATNOS-Ascan NOEs: UNIO ATNOS-Candid UNIO Candid ASDP Structure from NMR restraints: Ab initio: GeNMR Dynamo Cyana XPLOR-NIH ASDP UNIO ATNOS-Candid UNIO Candid Amber Fragment-based: BMRB CS-Rosetta Rosetta-NMR (Robetta) Template-based: GeNMR I-TASSER Refinement: Amber Structure from chemical shifts: Fragment-based: WeNMR CS-Rosetta BMRB CS-Rosetta Homology-based: CS23D Simshift Torsion angles from chemical shifts: TALOS Preditor Promega- Proline Secondary structure from chemical shifts: TALOS MICS caps, β-turns d2D PECAN CSI (via RCI server) Flexibility from chemical shifts: RCI Interactions from chemical shifts: HADDOCK Chemical shifts re-referencing: Shiftcor UNIO Shiftinspector LACS CheckShift RefDB NMR model quality: Agreement with distance restraints: PROSESS PSVS RPF scores iCing Agreement with chemical shifts: PROSESS CheShift2 Vasco iCing Agreement with RDCs: DC Anisofit Agreement with pseudocontact shifts: Anisofit Agreement with typical protein geomtery: Resolution-by-Proxy PROSESS What-If iCing PSVS MolProbity SAVES2 or SAVES4 Vadar Prosa ProQ MetaMQAPII PSQS Eval123D STAN Ramachandran Plot Rampage ERRAT Verify_3D Harmony Quality Control Check NMR spectrum prediction: FANDAS MestReS V-NMR Flexibility from structure: Backbone S2 Methyl S2 B-factor Molecular dynamics: Gromacs Amber Antechamber Chemical shifts prediction: From structure: Shiftx2 Sparta+ Camshift CH3shift- Methyl ArShift- Aromatic ShiftS Proshift PPM CheShift-2- Cα From sequence: Shifty Camcoil Poulsen_rc_CS Disordered proteins: MAXOCC Format conversion & validation: CCPN From NMR-STAR 3.1 Validate NMR-STAR 3.1 NMR sample preparation: Protein disorder: DisMeta Protein solubility: camLILA ccSOL Camfold camGroEL Zyggregator Isotope labeling: UPLABEL Solid-state NMR: sedNMR

» NMR Jobs Professor or Associate Professor in Biological NMR Spetroscopy at Aarhus University (874792) |... by nmrlearner Principal Analytical Chemist | Calhoun Vision, Inc. by nmrlearner Assistant, Associate or Full Professor of Pharmacognosy | The University of Mississippi, School of... by nmrlearner » Cool papers Including the Ensemble of Unstructured Conformations in the Analysis of Protein's Native State by... by nmrlearner NMR metabolomic modeling of age and lifespan: A multicohort analysis by nmrlearner Solution NMR chemical shift assignment of apo and molybdate-bound ModA at two pHs by nmrlearner » NMR books Solid State Properties of Pharmaceutical Materials by nmrlearner NMR Data Interpretation Explained: Understanding 1D and 2D NMR Spectra of Organic Compounds and... by nmrlearner Guide to Fluorine NMR for Organic Chemists, 2nd Edition by nmrlearner » NMR theses A Pulse NMR Trilogy: Anomalies, Anisotropies, and Adsorption by nmrlearner NMR imaging of solids with multiple-pulse line narrowing and radiofrequency gradients by nmrlearner Metabolic engineering applications of in vivo [superscript 31]P and [superscript 13]C NMR studies... by nmrlearner » NMR blogs New blog site by nmrlearner EasySpin Academy (online), Oct 13-15, 2020 by nmrlearner A compact X-Band ODNP spectrometer towards hyperpolarized 1H spectroscopy #DNPNMR #ODNP by nmrlearner » NMR news Optimal isotope labelling for NMR protein structure determinations - Nature.com by nmrlearner Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane... by nmrlearner Bruker Introduces Novel NMR Scientific Capabilities for Structural Biology Discoveries in Membrane... by nmrlearner » NMR tweets NMR tweet Hyperpolarized diamond nuclei increase NMR/MRI sensitivity: Today?s nuclear magnetic... by nmrlearner Hyperpolarized diamond nuclei increase NMR/MRI sensitivity: Today?s nuclear magnetic resonance... by nmrlearner iNMR enables you to process and visualize Nuclear Magnetic Resonance spectra. Have you been longing... by nmrlearner » NMR videos Jack Vale Interview | NMR Feature by nmrlearner NMR Made Easy! Part 6A - NMR to Molecule Structure - Organic Chemistry by nmrlearner Another introduction to proton NMR (9) by nmrlearner » NMR pictures butanone by nmrlearner ... temporal lobe epilepsy: a 1 H magnetic resonance spectroscopy study by nmrlearner Figure 12. Closed field magnetic resonance imaging machine [16]. by nmrlearner » Online Users: 326 0 members and 326 guests No Members online Most users ever online was 1,278, 01-09-2024 at 07:38 AM.

» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR Feb 29, 2024 - 10:00 PM - by nmrlearner Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR Abstract N-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where Xâ??â?*â??Proline) of the nascent protein. Oligosaccharyltransferase (OST), a multi-subunit membrane embedded enzyme catalyzes this glycosylation reaction in eukaryotes. In yeast, Ost4 is the smallest of nine subunits and bridges the interaction of the catalytic subunit, Stt3, with Ost3 (or its homolog, Ost6). Mutations of any C-terminal hydrophobic residues in Ost4 to a charged residue destabilizes the enzyme and negatively impacts its function. Specifically, the V23D mutation results in a temperature-sensitive phenotype in yeast. Here, we report the reconstitution of both purified recombinant Ost4 and Ost4V23D each in a POPC/POPE lipid bilayer and their resonance assignments using heteronuclear 2D and 3D solid-state NMR with magic-angle spinning. The chemical shifts of Ost4 changed significantly upon the V23D mutation, suggesting a dramatic change in its chemical environment. ... [Read More] 0 Replies | 117 Views NMR spectroscopy brings invisible protein states into focus - Nature.com Feb 29, 2024 - 10:00 PM - by nmrlearner NMR spectroscopy brings invisible protein states into focus - Nature.com NMR spectroscopy brings invisible protein states into focus Nature.com Read here 0 Replies | 80 Views [NMR paper] Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR Feb 29, 2024 - 10:00 PM - by nmrlearner Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR N-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ? Proline) of the nascent... More... 0 Replies | 78 Views Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com Feb 29, 2024 - 10:00 AM - by nmrlearner Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses Nature.com Read here 0 Replies | 92 Views [NMR paper] Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning Feb 29, 2024 - 10:00 AM - by nmrlearner Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning Deuterium rotating frame solid-state NMR relaxation measurements (²H R(1?)) are important tools in quantitative studies of molecular dynamics. We demonstrate how ²H to ^(13)C cross-polarization (CP) approaches under 10-40 kHz magic angle spinning rates can be combined with the ²H R(1?) blocks to allow for extension of deuterium rotating frame relaxation studies to methyl groups in biomolecules. This extension permits detection on the ^(13)C nuclei and, hence, for the achievement of site-specific... More... 0 Replies | 98 Views [NMR paper] Interactions between the protein barnase and co-solutes studied by NMR Feb 29, 2024 - 10:00 AM - by nmrlearner Interactions between the protein barnase and co-solutes studied by NMR Protein solubility and stability depend on the co-solutes present. There is little theoretical basis for selection of suitable co-solutes. Some guidance is provided by the Hofmeister series, an empirical ordering of anions according to their effect on solubility and stability; and by osmolytes, which are small organic molecules produced by cells to allow them to function in stressful environments. Here, NMR titrations of the protein barnase with Hofmeister anions and osmolytes are used to... More... 0 Replies | 60 Views [NMR paper] Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR Feb 29, 2024 - 10:00 AM - by nmrlearner Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR The coexistence of rigid and mobile molecules or molecular segments abounds in biomolecular assemblies. Examples include the carbohydrate-rich cell walls of plants and intrinsically disordered proteins that contain rigid ?-sheet cores. In solid-state nuclear magnetic resonance (NMR) spectroscopy, dipolar polarization transfer experiments are well suited for detecting rigid components, whereas scalar-coupling experiments are well suited for detecting highly mobile components. However, few NMR... More... 0 Replies | 69 Views Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid - ScienceDirect.com Feb 28, 2024 - 9:53 PM - by nmrlearner Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid - ScienceDirect.com Specific Interaction of the Human Mitochondrial Uncoupling Protein 1 with Free Long-Chain Fatty Acid ScienceDirect.com Read here 0 Replies | 79 Views Page 14 of 100 « First < 4 12 13 14 15 16 24 64 > Last »

» Search Forums » Advanced Search » BioNMR wiki NMR job online resources by nmrlearner List of Linux distributions that offer easy PyMol installation via a package manager by markber Compiling and installing MolMol under Linux from Louic S. Vermeer blog by markber » NMR discussion Journal Highlight: 3D printed NMR spectra: From 1D and 2D acquisition to 3D visualization by nmrlearner Golden probe: Opening the cluster by nmrlearner Journal Highlight: Explosive emulsion characterisation using nuclear magnetic resonance by nmrlearner » NMR conferences PANIC 2018 - Practical Applications of NMR in Industry Conference by nmrlearner Practical Applications of NMR in Industry Conference (PANIC) by nmrlearner PANIC 2016 - Practical Applications of NMR in Industry Conference by nmrlearner » NMR software A source of RPMs for several NMR-related programs by nmrlearner New File Added: GARANT by Chris. Bart. Basics of Mathematica to Understand POMA by nmrlearner » Pulse sequences Pulse programs by sivanmr Bruker GFT-NMR pulse programs from Dr. Gao website by nmrlearner BMRB library of Bruker pulse sequences by nmrlearner » NMR community NMR Group of Gary Shaw at the University of Western Ontario by mrevingt "Eureka moments" push U of A researcher to top publishing spot - The Gateway Online by nmrlearner Prof. Konstantin Pervushin's NMR group by markber » NMR presentations Humans, aliens, and eHarmony or why there is no such thing as a free lunch in protein structure... by markber » NMR web resources NMR and MRI related tools and short programs by nmrlearner Advanced NMR Spectroscopy by nmrlearner Understanding NMR Spectroscopy - The Solutions and Figures by nmrlearner » NMR feature requests Are you interested in further development of PPT-DB: the protein property prediction and testing... by markber » NMR bookmarks Aldrich library of NMR spectra by nmrlearner NMR spectra of polymers and polymer additives by nmrlearner nmr basics by nmrlearner » Stats Members: 3,202 Threads: 25,741 Posts: 26,127 Top Poster: nmrlearner (23,185) Welcome to our newest member, bpadmanabhan