[Question from NMRWiki Q&A forum] Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
I am facing problems in calibration of my HCCH-TOCSY spectrum in reference to HBHACONH and HCCCONH spectrum. Can somebody tell me how to solve this issue? Can I autoassign my HCCH-TOCSY spectrum using HBHACONH spectrum
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10-02-2011 08:25 AM
4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins
4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins
Abstract A four-dimensional (4D) APSY (automated projection spectroscopy)-HBCB(CG)CDHD experiment is presented. This 4D experiment correlates aromatic with aliphatic carbon and proton resonances from the same amino acid side chain of proteins in aqueous solution. It thus allows unambiguous sequence-specific assignment of aromatic amino acid ring signals based on backbone assignments. Compared to conventional 2D approaches, the inclusion of evolution periods on 1Hβ and 13Cδ...
[NMR paper] Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(1
Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(13)C groups in proteins.
Related Articles Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(13)C groups in proteins.
J Magn Reson. 1999 Aug;139(2):447-50
Authors: Meissner A, Sorensen OW
Improved methods for three-dimensional TROSY-Type HCCH correlation involving protons of negligible CSA are presented. The TROSY approach differs from the conventional approach of heteronuclear decoupling in evolution and detection periods by...
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11-18-2010 08:31 PM
[Question from NMRWiki Q&A forum] HCCH-Tocsy connectivities
HCCH-Tocsy connectivities
What I see from this picture (linked from www.protein-nmr.org.uk):
http://www.protein-nmr.org.uk/pictures/experiment_types/hcch_tocsy.png
that shows an HCCH-TOCSY magnetisation transfer I do not see any transfer from HD to HB, but in my peaks I see a peak that seems it can be connected az Leu CB-HB2-HD22Can I have these peaks in HCCH-TOCSY?
would you please introduce me a reference that tell me what kind of connection can be visible in HC(C)H-Tocsy
[Nature network NMR forum] HCCH-TOCSY (0 replies)
HCCH-TOCSY (0 replies)
Hello everybody,
I am a biologist who has jumped into NMR :(
I hope this is an active forum so that I can improve myself and can help someday to others.
I have a very stupid question!!! Do you know if I can see some HN peaks correlated with C-beta in HCCH-TOCSY?
Cheers,