NMRwiki tweet nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled samp

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[NMRwiki tweet] nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled samp

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08-22-2010, 01:49 AM

nmrlearner

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nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled samp

nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/

nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/

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[Question from NMRWiki Q&A forum] Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe? Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe? If I'm running relaxation experiments (15N) for R1, R2, and hetnoe on a double labeled (2H/15N) sample, do I need to do any deuterium decoupling added to the program? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	08-22-2010 02:30 AM
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Structure determination of proteins in 2H2O solution aided by a deuterium-decoupled 3 Abstract We developed an NMR pulse sequence, 3D HCA(N)CO, to correlate the chemical shifts of protein backbone 1HÎ± and 13CÎ± to those of 13Câ?² in the preceding residue. By applying 2H decoupling, the experiment was accomplished with high sensitivity comparable to that of HCA(CO)N. When combined with HCACO, HCAN and HCA(CO)N, the HCA(N)CO sequence allows the sequential assignment using backbone 13Câ?² and amide 15N chemical shifts without resort to backbone amide protons. This assignment strategy was demonstrated for 13C/15N-labeled GB1 dissolved in 2H2O. The quality of the GB1 structure...	nmrlearner	Journal club	0	08-14-2010 04:19 AM

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