CBCANH experment on 700 vnmr
Iam working on protein family which can form dimers (total 20kd size ) using 25mM TRIS , 100 mM KCL Ph-6.8 , protein conc -2mM ( limitaion )
Iam always geting bad quality of CBCANH contures experment on 700 vnmr cryob .
quality of N-H hsqc is good
Could you please suggest me about expermental parameters or buffer conditions to get good quality of CBCANH experment
penumutchu.srinivas
NMR Questions and Answers
2
11-15-2011 11:46 AM
[Question from NMRWiki Q&A forum] dmf problem in vnmr
dmf problem in vnmr
Hi, dear all,
I try to use garp1 sequence for decoupling with the statement " obsprgon("garp1", pw, angle, 0) ".
If angle = 1.0, when pw = 4us or larger than 4us, the sequence works. When pw less that 4us, it shows the error "effective dmf too high prg dec on on controller dec".
If angle = 2.0, when pw = 2us or larger than 2us, it works. When pw less that 2us, the it shows the same error message.
nmrlearner
News from other NMR forums
0
06-27-2011 04:31 PM
2D processing in VNMR
2D processing in VNMR
Basic Processing, Display and Plotting of 2D Spectra in VNMR
More...
nmrlearner
General
0
05-27-2011 10:44 PM
3d NMR data processing on vnmr
Q-1. Could you pls suggest me that default or reference Linear Prediction parameters of following to enter to process 3d nmr in t1 t2 i need to enter following parameters on vnmrj
coef -
basic pts -
starting at -
predicted pts -
starting at -
while iam processing data it giving error messasge as value for Strtlp is too small
Q-2. weighting functions which can we prefer more frquntely to process 3d nmr on VNMRJ ?
[Question from NMRWiki Q&A forum] What is the easiest way to emulate the "array" command from VNMR in XWINNMR/TOPSPIN?
What is the easiest way to emulate the "array" command from VNMR in XWINNMR/TOPSPIN?
What is the easiest way to emulate the "array" command from VNMR in XWINNMR/TOPSPIN? I recently moved from a lab using a Varian (Unity plus 500) with VNMR 6.whatever to a lab with only Bruker (AVANCE 300/400/500) spectrometers available. I am used to using the "array" caommand in VNMR and viewing the spectra with "dssa" or "dssh." I don't know a convenient way to do the same thing in XWINNMR. I know there are some AU programs but I'm not sure what they all do or if what I want is available. I tried...
nmrlearner
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09-24-2010 07:36 PM
[Question from NMRWiki Q&A forum] Vnmr: What to do when explib command does not work?
Vnmr: What to do when explib command does not work?
Hello, in one of accounts on our system explib command does not work but works fine in other accounts.
explib lists experiments loaded into the given user space and shows which are queued or running.
Is there something I can do on the file system - maybe fix/delete some file or record to make it work again?
Thanks.
nmrlearner
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0
08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] How to set up 15N direct detection on UnityInova?
How to set up 15N direct detection on UnityInova?
Has anyone recorded directly detected 15N spectra on UnityInova? We have a triple resonance HCN probe. The sample I've used was natural abundance 1M NaNO3 - sodium nitrate.
I've tried to set up cable analogously to 13C detection - through the second preamp, but could not see any signal at all.
Check if somebody has answered this question on NMRWiki QA forum
nmrwiki: How to fix explib command in vnmr? we have a unityinova #nmr spectrometer. T
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