Pf1 Phage - BioNMR

Has anyone tried to use Pf1 phage to align their sample and collect RDCs? I've tried to use it several times. At first, I had trouble getting consistent alignment with each sample. I thought that was mainly due to pipetting errors since the phage is so viscous. Next, I used the teflon tubing that Asla Biochem suggests, and now with 8 mg/mL of phage in my sample, I am not seeing any splitting in the D2O spectrum. I can't add more phage as it is too expensive and will start to interact with my protein.

Does anyone out there have any tips or secrets to working with phage? I've searched extensively online, and no one seems to have much trouble with it. In fact, everyone says its a great way to create alignment.

Thanks,

Jenn

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