xTract: software for characterizing conformational changes of protein ... - Nature.com
xTract: software for characterizing conformational changes of protein ... - Nature.com
Nature.com xTract: software for characterizing conformational changes of protein... Nature.com
Chemical cross-linking in combination with mass spectrometry generates distance restraints of amino acid pairs in close proximity on the surface of native proteins and protein complexes. ..... with mass spectrometry (CX-MS) is increasingly being used ...
Protein engineering: The power of four - Nature.com
Protein engineering: The power of four - Nature.com
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Protein engineering: The power of four
Nature.com
The final structure of the dimer of dimers, which they called 'rocker', was characterized by X-ray crystallography, and a variety of NMR techniques, which, in combination with molecular dynamic simulations, indicated that the peptide assembly did ...
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03-24-2015 09:58 PM
[NMR paper] Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
Methods Mol Biol. 2015;1229:325-33
Authors: Joseph PR, Poluri KM, Sepuru KM, Rajarathnam K
Abstract
Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for characterizing the binding interface of macromolecular complexes. (1)H-(15) N-HSQC-based CSP studies have...
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10-19-2014 01:27 PM
[NMR paper] Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction.
Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction.
Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction.
Biomacromolecules. 2013 Sep 11;
Authors: Sampath S, Jenkins JE, Butler E, Kim J, Henning RW, Holland GP, Yarger JL
Abstract
This study provides a detailed secondary structural characterization of major ampullate dragline silk from Latrodectus hesperus (black widow) spiders. X-ray diffraction results show...
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09-13-2013 12:05 PM
[NMR paper] Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Characterizing carbohydrate-protein interactions by NMR.
Biopolymers. 2013 Jun 20;
Authors: Bewley CA, Shahzad-Ul-Hussan S
Abstract
Interactions between proteins and soluble carbohydrates and/or surface displayed glycans are central to countless recognition, attachment and signaling events in biology. The physical chemical features associated with these binding events vary considerably, depending on the biological system of interest. For example, carbohydrate-protein...
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06-21-2013 01:10 PM
[NMR paper] Solid State NMR Strategy for Characterizing Native Membrane Protein Structures.
Solid State NMR Strategy for Characterizing Native Membrane Protein Structures.
Related Articles Solid State NMR Strategy for Characterizing Native Membrane Protein Structures.
Acc Chem Res. 2013 Mar 7;
Authors: Murray DT, Das N, Cross TA
Abstract
Unlike water soluble proteins, the structures of helicaltransmembrane proteins depend on a very complex environment. These proteins sit in the midst of dramatic electrical and chemical gradients and are often subject to variations in the lateral pressure profile, order parameters, dielectric...
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03-09-2013 11:05 AM
Lysine methylation strategies for characterizing protein conformations by NMR
Lysine methylation strategies for characterizing protein conformations by NMR
Abstract In the presence of formaldehyde and a mild reducing agent, reductive methylation is known to achieve near complete dimethylation of protein amino groups under non-denaturing conditions, in aqueous media. Amino methylation of proteins is employed in mass spectrometric, crystallographic, and NMR studies. Where biosynthetic labeling is prohibitive, amino 13C-methylation provides an attractive option for monitoring folding, kinetics, proteinā??protein and protein-DNA interactions by NMR. Here, we...
xTract: software for characterizing conformational changes of protein ... - Nature.com
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