Validating hits through NMR as an orthogonal biophysical method - News-Medical.net

		BioNMR > NMR community > Online News
Validating hits through NMR as an orthogonal biophysical method - News-Medical.net

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-03-2017, 08:50 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Validating hits through NMR as an orthogonal biophysical method - News-Medical.net

News-Medical.net

Validating hits through NMR as an orthogonal biophysical method
News-Medical.net
It can therefore be complemented with the use of Nuclear Magnetic Resonance (NMR), an orthogonal biophysical method that can detect target-ligand interactions with high sensitivity but that necessitates a high consumption of protein compared to other ...

Validating hits through NMR as an orthogonal biophysical method - News-Medical.net
More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
What is Structural Genomics? - News-Medical.net http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcTc7BTZDAqNY9IP0XKaT3YgQ5rHzjRoKeElI2zGkLtcvYg2WazMaMDnRUTChYpJ8Ma5z9lytBh8 News-Medical.net <img alt="" height="1" width="1"> What is Structural Genomics? News-Medical.net The purified and crystallized proteins can be analyzed with X-ray crystallography or Nuclear Magnetic Resonance. This allows the structure of every protein encoded by the genome to be determined. Ab initio modeling: information about the protein ... What is Structural Genomics? - News-Medical.net	nmrlearner	Online News	0	03-09-2017 12:11 AM
Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins - News-Medical.net Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins - News-Medical.net http://www.bionmr.com//t2.gstatic.com/images?q=tbn:ANd9GcSjxWhOWctvAA_L-9UDE3ufkiT94K_xq-P-YeTc-09I3x4H04SiM6ADLdY2zBipCXjujvZLEBQy News-Medical.net <img alt="" height="1" width="1"> Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins News-Medical.net Structural studies like nuclear magnetic resonance (NMR) and X-ray crystallography provide additional validation and characterization. This article describes the...	nmrlearner	Online News	0	11-19-2016 08:35 PM
Protein allostery and NMR - News-Medical.net Protein allostery and NMR - News-Medical.net http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcSUYWi2bHelN_RTBhh7qYfmfflIRmDxiYVSzcgPFRjATNYZC8QP-GVT_0mnQ_aBj29OydIJOPJ6 News-Medical.net <img alt="" height="1" width="1"> Protein allostery and NMR News-Medical.net Allostery â?? the phenomenon by which an event in one part of a molecule causes an effect in another â?? is a key feature of protein regulation in all living cells. Rather than directly affecting a protein's active site, allostery can modulate the active ...	nmrlearner	Online News	0	08-03-2016 05:31 PM
Automated Method for Purifying Protein:Ligand Complexes - News-Medical.net Automated Method for Purifying Protein:Ligand Complexes - News-Medical.net http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcQutdD6NnjJDnC9but3zjmMB4bBDLXFw5LX7xJfJWUzs2t8oAX-oS63Npooi2n9ra6w4V88Fp8j News-Medical.net <img alt="" height="1" width="1"> Automated Method for Purifying Protein:Ligand Complexes News-Medical.net Common techniques for further analyzing protein:ligand complexes to gain structural information include X-ray crystallography, NMR, and cryo-EM. However, a lack of reproducible and robust separation techniques means the purification of protein:ligand ...	nmrlearner	Online News	0	05-20-2015 10:27 AM
Researchers find new method to measure modified protein structures in ... - News-Medical.net <img alt="" height="1" width="1" /> Researchers find new method to measure modified protein structures in ... News-Medical.net Although there is a series of techniques to study structurally modified proteins, such as x-ray crystallography, nuclear magnetic resonance spectroscopy and other spectroscopic techniques, they cannot be used to analyse complex biological samples. Researchers find new method to measure modified protein structures in ... - News-Medical.net More...	nmrlearner	Online News	0	09-16-2014 08:38 PM
New solid state NMR method can help visualize structure of proteins - News-Medical.net New solid state NMR method can help visualize structure of proteins - News-Medical.net <img alt="" height="1" width="1" /> New solid state NMR method can help visualize structure of proteins News-Medical.net For roughly a decade, a technique called solid state nuclear magnetic resonance (NMR) spectroscopy has allowed researchers to detect the arrangements of atoms in proteins that defy study by traditional laboratory tools such as X-ray crystallography. and more » Read here	nmrlearner	Online News	0	03-20-2012 10:37 AM
Cotten, Baxter '11 and McGavin '12 Publish in Biophysical Journal - Hamilton College News http://nt0.ggpht.com/news/tbn/LA9z4IqqV7KCdM/6.jpg Hamilton College News <img alt="" height="1" width="1" /> Cotten, Baxter '11 and McGavin '12 Publish in Biophysical Journal Hamilton College News In the summer of 2010, Baxter and McGavin spent 10 days working at Opella's laboratory, the Center for Nuclear Magnetic Resonance (NMR) Spectroscopy and Imaging of Proteins at UCSD. There they learned how to make bicelles (â??bilayered micellesâ??), ... Cotten, Baxter '11 and McGavin '12 Publish in Biophysical Journal - Hamilton College News	nmrlearner	Online News	0	09-16-2011 10:01 AM
Biophysical Society names 2011 award recipients - Genetic Engineering News (press rel Biophysical Society names 2011 award recipients - Genetic Engineering News (press release) <img alt="" height="1" width="1" /> Biophysical Society names 2011 award recipients Genetic Engineering News (press release) ... membrane protein biochemistry. Eric Oldfield, University of Illinois, will be awarded the Avanti Award in Lipids for his pioneering research using NMR ... and more » Read here	nmrlearner	Online News	0	09-04-2010 06:53 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off