Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins - News-Medical.net

		BioNMR > NMR community > Online News
Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins - News-Medical.net

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-19-2016, 08:35 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins - News-Medical.net

Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins - News-Medical.net

News-Medical.net

Using Isothermal Titration Calorimetry for Biophysical Characterization of Chromatin-Binding Proteins
News-Medical.net
Structural studies like nuclear magnetic resonance (NMR) and X-ray crystallography provide additional validation and characterization. This article describes the features of the ITC assay and shows how ITC can be used to determine the KD of binding ...

Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Using NMR to investigate intrinsically disordered proteins: an interview with Dr Isabella Felli - News-Medical.net Using NMR to investigate intrinsically disordered proteins: an interview with Dr Isabella Felli - News-Medical.net <img alt="" height="1" width="1"> Using NMR to investigate intrinsically disordered proteins: an interview with Dr Isabella Felli News-Medical.net This mainstream understanding kept the scientific community distracted from also focusing on a lot of other important proteins, such as the more flexible ones. We all know how NMR spectroscopy is not only used to look at structural information, but can ... and more » Read here	nmrlearner	Online News	0	07-01-2016 03:06 AM
Unlocking intrinsically disordered proteins: an interview with Peter Wright - News-Medical.net Unlocking intrinsically disordered proteins: an interview with Peter Wright - News-Medical.net http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcQ0HUfsDqvUbSqQDsGm-tqskmSpF8YqwwODycyDO5HBNq0_aUwY-DX3k5_NRC5gMJjgYy2R33c News-Medical.net <img alt="" height="1" width="1"> Unlocking intrinsically disordered proteins: an interview with Peter Wright News-Medical.net I'm a professor in the Department of Integrative Structural and Computational Biology at The Scripps Research Institute. I have been performing NMR research on proteins for nearly 40 years. In very general terms, my...	nmrlearner	Online News	0	02-11-2016 10:30 PM
Using ITC Technique for Characterization of Protein-Protein Interactions - News-Medical.net Using ITC Technique for Characterization of Protein-Protein Interactions - News-Medical.net http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcSPV3efNTb3XMRYliY8vnCW7qC7xNsSnkJLHKLQbyk7-ZGnhpI_1OC_nTXOAGrrAU5GSwVsiSRz News-Medical.net <img alt="" height="1" width="1"> Using ITC Technique for Characterization of Protein-Protein Interactions News-Medical.net With each interaction, the protein structural changes occur through specific electrostatic and van der Waals interactions, the breaking and formation of hydrogen bonds and the burial of surfaces exposed to solvents. It is...	nmrlearner	Online News	0	06-24-2015 01:08 PM
[NMR paper] Electrostatic interactions in the binding pathway of a transient protein complex studied by NMR and isothermal titration calorimetry. Electrostatic interactions in the binding pathway of a transient protein complex studied by NMR and isothermal titration calorimetry. Related Articles Electrostatic interactions in the binding pathway of a transient protein complex studied by NMR and isothermal titration calorimetry. J Biol Chem. 2014 Aug 13; Authors: Meneses E, Mittermaier A Abstract Much of our knowledge of protein binding pathways is derived from extremely stable complexes that interact very tightly, with lifetimes of hours to days. Much less is known about...	nmrlearner	Journal club	0	08-15-2014 12:53 PM
Researchers solve complete 3-D structure of E6 proteins in HPV 16 - News-Medical.net Researchers solve complete 3-D structure of E6 proteins in HPV 16 - News-Medical.net <img alt="" height="1" width="1" /> Researchers solve complete 3-D structure of E6 proteins in HPV 16 News-Medical.net Success depended on two factors: the development of methods for isolating protein E6 as well as the use of nuclear magnetic resonance (NMR) and crystallography techniques. Having dealt with various aggregation and purification problems, the researchers ... and more » Read here	nmrlearner	Online News	0	02-16-2013 05:53 AM
New solid state NMR method can help visualize structure of proteins - News-Medical.net New solid state NMR method can help visualize structure of proteins - News-Medical.net <img alt="" height="1" width="1" /> New solid state NMR method can help visualize structure of proteins News-Medical.net For roughly a decade, a technique called solid state nuclear magnetic resonance (NMR) spectroscopy has allowed researchers to detect the arrangements of atoms in proteins that defy study by traditional laboratory tools such as X-ray crystallography. and more » Read here	nmrlearner	Online News	0	03-20-2012 10:37 AM
Supramolecular interactions between losartan and hydroxypropyl-?-CD: ESI mass-spectrometry, NMR techniques, phase solubility, isothermal titration calorimetry and anti-hypertensive studies. Supramolecular interactions between losartan and hydroxypropyl-?-CD: ESI mass-spectrometry, NMR techniques, phase solubility, isothermal titration calorimetry and anti-hypertensive studies. Supramolecular interactions between losartan and hydroxypropyl-?-CD: ESI mass-spectrometry, NMR techniques, phase solubility, isothermal titration calorimetry and anti-hypertensive studies. Int J Pharm. 2011 Feb 14;404(1-2):116-23 Authors: de Paula WX, Denadai AM, Santoro MM, Braga AN, Santos RA, Sinisterra RD In this work, low soluble supramolecular complex...	nmrlearner	Journal club	0	04-13-2011 11:57 PM
Purifying Proteins: Researchers Use NMR To Improve Drug Development - Medical News To Purifying Proteins: Researchers Use NMR To Improve Drug Development - Medical News Today (press release) <img alt="" height="1" width="1" /> Purifying Proteins: Researchers Use NMR To Improve Drug Development Medical News Today (press release) From the NMR data, they were able to determine what part and type of the protein the ligands were binding to and how strongly they would bind. ... and more » Read here	nmrlearner	Online News	0	10-26-2010 08:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off