Up Close with Membrane Lipid-Protein Complexes - Science Careers Blog (subscription)

		BioNMR > NMR community > Online News
Up Close with Membrane Lipid-Protein Complexes - Science Careers Blog (subscription)

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-21-2011, 09:52 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Up Close with Membrane Lipid-Protein Complexes - Science Careers Blog (subscription)

Up Close with Membrane Lipid-Protein Complexes - Science Careers Blog (subscription)

Up Close with Membrane Lipid-Protein Complexes
Science Careers Blog (subscription)
(2) report mass spectrometry of intact integral membrane protein complexes solubilized from bilayers. The results show that specific structural lipids remain bound in the gas phase and can be counted. Despite some technical hurdles, integral membrane ...

Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy wit Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy with the use of paramagnetic reagents. Related Articles Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy with the use of paramagnetic reagents. Chembiochem. 2004 Apr 2;5(4):467-73 Authors: Hilty C, Wider G, Fernández C, Wüthrich K For solution NMR studies of the structure and function of membrane proteins, these macromolecules have to be reconstituted and solubilized in detergent micelles. Detailed characterization of the mixed...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Lipid-protein interactions in DHPC micelles containing the integral membrane protein Lipid-protein interactions in DHPC micelles containing the integral membrane protein OmpX investigated by NMR spectroscopy. Related Articles Lipid-protein interactions in DHPC micelles containing the integral membrane protein OmpX investigated by NMR spectroscopy. Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13533-7 Authors: Fernández C, Hilty C, Wider G, Wüthrich K Intermolecular nuclear Overhauser effects (NOEs) between the integral outer membrane protein OmpX from Escherichia coli and dihexanoylphosphatidylcholine (DHPC) provided a detailed...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative analysis of the lipid extract of the purple membrane. Related Articles Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative analysis of the lipid extract of the purple membrane. J Lipid Res. 2002 Jan;43(1):132-40 Authors: Corcelli A, Lattanzio VM, Mascolo G, Papadia P, Fanizzi F The lipid/protein stoichiometries of a naturally crystalline biological membrane, the purple membrane (PM) of Halobacterium salinarum, have been...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
Exciting Structures - Securities Industry News (blog) (subscription) Exciting Structures - Securities Industry News (blog) (subscription) <img alt="" height="1" width="1" /> Exciting Structures Securities Industry News (blog) (subscription) ... to determine the high-resolution structure of excited proteins using conventional techniques such as nuclear magnetic resonance (NMR) spectroscopy and ... Read More	nmrlearner	Online News	0	09-10-2010 12:48 AM
[NMR paper] 13C-NMR studies of membrane lipid-protein interactions upon protein heat denaturation 13C-NMR studies of membrane lipid-protein interactions upon protein heat denaturation. Related Articles 13C-NMR studies of membrane lipid-protein interactions upon protein heat denaturation. J Biochem Biophys Methods. 1991 Oct-Nov;23(3):259-61 Authors: YashRoy RC Spinach chloroplast membranes were studied by natural abundance carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy in their normal state and after heat denaturation of membrane proteins. The membrane proteins were denatured by raising the temperature of the sample to 67...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] 13C-NMR studies of membrane lipid-protein interactions upon protein heat denaturation 13C-NMR studies of membrane lipid-protein interactions upon protein heat denaturation. Related Articles 13C-NMR studies of membrane lipid-protein interactions upon protein heat denaturation. J Biochem Biophys Methods. 1991 Oct-Nov;23(3):259-61 Authors: YashRoy RC Spinach chloroplast membranes were studied by natural abundance carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy in their normal state and after heat denaturation of membrane proteins. The membrane proteins were denatured by raising the temperature of the sample to 67...	nmrlearner	Journal club	0	08-21-2010 11:12 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off