Unveiling invisible protein states with NMR spectroscopy - ScienceDirect

		BioNMR > NMR community > Online News
Unveiling invisible protein states with NMR spectroscopy - ScienceDirect

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-28-2023, 10:50 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,599

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Unveiling invisible protein states with NMR spectroscopy - ScienceDirect

Unveiling invisible protein states with NMR spectroscopy - ScienceDirect

Unveiling invisible protein states with NMR spectroscopy ScienceDirect Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
NMR probing of invisible excited states using selectively labeled RNAs NMR probing of invisible excited states using selectively labeled RNAs Abstract Carrâ??Purcellâ??Meiboomâ??Gill (CPMG) relaxation dispersion NMR experiments are invaluable for probing sparsely and transiently populated biomolecular states that cannot be directly detected by traditional NMR experiments and that are invisible by other biophysical approaches. A notable gap for RNA is the absence of CPMG experiments for measurement of methine base 1H and methylene C5â?² chemical shifts of ribose moieties in the excited state, partly because of...	nmrlearner	Journal club	0	06-02-2018 12:51 AM
[NMR paper] Studying "Invisible" Excited Protein States in Slow Exchange with a Major State Conformation. From Mendeley Biomolecular NMR group: Studying "Invisible" Excited Protein States in Slow Exchange with a Major State Conformation. Journal of the American Chemical Society (2012). Pramodh Vallurupalli, Guillaume Bouvignies, Lewis E Kay et al. Ever since its initial development, solution NMR spectroscopy has been used as a tool to study conformational exchange. Although many systems are amenable to relaxation dispersion approaches, cases involving highly skewed populations in slow chemical exchange have, in general, remained recalcitrant to study. Here an experiment to detect and...	nmrlearner	Journal club	0	10-17-2013 12:49 PM
[NMR paper] Studying "Invisible" Excited Protein States in Slow Exchange with a Major State Conformation. From Mendeley Biomolecular NMR group: Studying "Invisible" Excited Protein States in Slow Exchange with a Major State Conformation. Journal of the American Chemical Society (2012). Pramodh Vallurupalli, Guillaume Bouvignies, Lewis E Kay et al. Ever since its initial development, solution NMR spectroscopy has been used as a tool to study conformational exchange. Although many systems are amenable to relaxation dispersion approaches, cases involving highly skewed populations in slow chemical exchange have, in general, remained recalcitrant to study. Here an experiment to detect and...	nmrlearner	Journal club	0	11-12-2012 01:53 AM
[NMR paper] Studying "Invisible" Excited Protein States in Slow Exchange with a Major State Conformation. From Mendeley Biomolecular NMR group: Studying "Invisible" Excited Protein States in Slow Exchange with a Major State Conformation. Journal of the American Chemical Society (2012). Pramodh Vallurupalli, Guillaume Bouvignies, Lewis E Kay et al. Ever since its initial development, solution NMR spectroscopy has been used as a tool to study conformational exchange. Although many systems are amenable to relaxation dispersion approaches, cases involving highly skewed populations in slow chemical exchange have, in general, remained recalcitrant to study. Here an experiment to detect and...	nmrlearner	Journal club	0	10-12-2012 09:58 AM
Measuring (1)H (N) temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy. Measuring (1)H (N) temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy. Measuring (1)H (N) temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy. J Biomol NMR. 2011 Mar 18; Authors: Bouvignies G, Vallurupalli P, Cordes MH, Hansen DF, Kay LE A method based on the Carr-Purcell-Meiboom-Gill relaxation dispersion experiment is presented for measuring the temperature coefficients of amide proton chemical shifts of low populated 'invisible' protein states that exchange...	nmrlearner	Journal club	0	03-23-2011 05:41 PM
Measuring 1HN temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy Measuring 1HN temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy Abstract A method based on the Carr-Purcell-Meiboom-Gill relaxation dispersion experiment is presented for measuring the temperature coefficients of amide proton chemical shifts of low populated â??invisibleâ?? protein states that exchange with a â??visibleâ?? ground state on the millisecond time-scale. The utility of the approach is demonstrated with an application to an I58D mutant of the Pfl6 Cro protein that undergoes exchange between the native, folded state and a cold...	nmrlearner	Journal club	0	03-22-2011 07:32 PM
[NMR paper] Reconstructing NMR spectra of "invisible" excited protein states using HSQC and HMQC Reconstructing NMR spectra of "invisible" excited protein states using HSQC and HMQC experiments. Related Articles Reconstructing NMR spectra of "invisible" excited protein states using HSQC and HMQC experiments. J Am Chem Soc. 2002 Oct 16;124(41):12352-60 Authors: Skrynnikov NR, Dahlquist FW, Kay LE Carr-Purcell-Meiboom-Gill (CPMG) relaxation measurements employing trains of 180 degrees pulses with variable pulse spacing provide valuable information about systems undergoing millisecond-time-scale chemical exchange. Fits of the CPMG relaxation...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
Using relaxation dispersion NMR spectroscopy to determine structures of excited, invisible protein states Using relaxation dispersion NMR spectroscopy to determine structures of excited, invisible protein states D. Flemming Hansen, Pramodh Vallurupalli and Lewis E. Kay Journal of Biomolecular NMR; 2008; 41(3); pp 113 - 120 Abstract: Currently the main focus of structural biology is the determination of static three-dimensional representations of biomolecules that for the most part correspond to low energy (ground state) conformations. However, it is becoming increasingly well recognized that higher energy structures often play important roles in function as well. Because these conformers...	daniel	Journal club	0	08-03-2008 03:16 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off