[NMR paper] Quantification of metabolites by NMR spectroscopy in the presence of protein.
Quantification of metabolites by NMR spectroscopy in the presence of protein.
Related Articles Quantification of metabolites by NMR spectroscopy in the presence of protein.
J Proteome Res. 2017 Mar 15;:
Authors: Wallmeier J, Samol C, Ellmann L, Zacharias HU, Vogl FC, Garcia M, Dettmer K, Oefner PJ, Gronwald W
Abstract
The high reliability of NMR spectroscopy makes it an ideal tool for large-scale metabolomic studies. However, the complexity of biofluids and, in particular, the presence of macromolecules poses a significant...
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03-16-2017 06:36 PM
[NMR paper] CH3-specific NMR assignment of alanine, isoleucine, leucine and valine methyl groups in high molecular weight proteins using a single sample.
CH3-specific NMR assignment of alanine, isoleucine, leucine and valine methyl groups in high molecular weight proteins using a single sample.
Related Articles CH3-specific NMR assignment of alanine, isoleucine, leucine and valine methyl groups in high molecular weight proteins using a single sample.
J Biomol NMR. 2015 Nov 13;
Authors: Kerfah R, Hamelin O, Boisbouvier J, Marion D
Abstract
A new strategy for the NMR assignment of aliphatic side-chains in large perdeuterated proteins is proposed. It involves an alternative isotopic...
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11-15-2015 07:55 PM
CH 3 -specific NMR assignment of alanine, isoleucine, leucine and valine methyl groups in high molecular weight proteins using a single sample
CH 3 -specific NMR assignment of alanine, isoleucine, leucine and valine methyl groups in high molecular weight proteins using a single sample
Abstract
A new strategy for the NMR assignment of aliphatic side-chains in large perdeuterated proteins is proposed. It involves an alternative isotopic labeling protocol, the use of an out-and-back 13Cā??13C TOCSY experiment ((H)C-TOCSY-C-TOCSY-(C)H) and an optimized non-uniform sampling protocol. It has long been known that the non-linearity of an aliphatic spin-system (for example Ile, Val, or Leu)...
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11-14-2015 03:37 PM
In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
From The DNP-NMR Blog:
In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
Schmidt, R., et al., In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding. J Magn Reson, 2014. 240(0): p. 8-15.
http://www.ncbi.nlm.nih.gov/pubmed/24486720
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06-02-2014 10:02 PM
Students dive into 3-D proteins - Mother Nature Network
Students dive into 3-D proteins - Mother Nature Network
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Students dive into 3-D proteins
Mother Nature Network
For decades, with funding from the National Science Foundation (NSF), Hinton has used nuclear magnetic resonance (NMR) to look at protein structure and function. But he wanted to find a way to educate and engage students about his discoveries. ...
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Systems NMR: single-sample quantification of RNA, proteins and metabolites for biomolecular network analysis - Nature.com
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