Systems-level Thinking Helps to Address Protein Folding - Institute for Ethics and Emerging Technologies
Systems-level Thinking Helps to Address Protein Folding - Institute for Ethics and Emerging Technologies
Systems-level Thinking Helps to Address Protein Folding Institute for Ethics and Emerging Technologies
There has been much technological advance in determining the static and dynamic features of protein structures, including in X-ray crystallography, NMR (nuclear magnetic resonance spectroscopy), cryo-electron microscopy, small-angle X-ray scattering ...
A 2D 13C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding
A 2D 13C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding
Abstract A 2D 13C Chemical Exchange Saturation Transfer (CEST) experiment is presented for studying slowly exchanging protein systems using methyl groups as probes. The utility of the method is first established through studies of protein L, a small protein, for which chemical exchange on the millisecond time-scale is not observed. Subsequently the approach is applied to a folding exchange reaction of a G48M mutant Fyn SH3 domain, for which only cross-peaks...
nmrlearner
Journal club
0
06-16-2012 06:01 AM
[NMR paper] An NMR view of the folding process of a CheY mutant at the residue level.
An NMR view of the folding process of a CheY mutant at the residue level.
Related Articles An NMR view of the folding process of a CheY mutant at the residue level.
Structure. 2002 Sep;10(9):1173-1185
Authors: Garcia P, Serrano L, Rico M, Bruix M
The folding of CheY mutant F14N/V83T was studied at 75 residues by NMR. Fluorescence, NMR, and sedimentation equilibrium studies at different urea and protein concentrations reveal that the urea-induced unfolding of this CheY mutant includes an on-pathway molten globule-like intermediate that can...
nmrlearner
Journal club
0
11-24-2010 08:58 PM
[NMR paper] Dynamic activation of protein function: a view emerging from NMR spectroscopy.
Dynamic activation of protein function: a view emerging from NMR spectroscopy.
Related Articles Dynamic activation of protein function: a view emerging from NMR spectroscopy.
Nat Struct Biol. 2001 Nov;8(11):926-31
Authors: Wand AJ
Recent developments in solution NMR methods have allowed for an unprecedented view of protein dynamics. Current insights into the nature of protein dynamics and their potential influence on protein structure, stability and function are reviewed. Particular emphasis is placed on the potential of fast side chain motion...
nmrlearner
Journal club
0
11-19-2010 08:44 PM
[NMR paper] Apoflavodoxin (un)folding followed at the residue level by NMR.
Apoflavodoxin (un)folding followed at the residue level by NMR.
Related Articles Apoflavodoxin (un)folding followed at the residue level by NMR.
Protein Sci. 2000 Jan;9(1):145-57
Authors: van Mierlo CP, van den Oever JM, Steensma E
The denaturant-induced (un)folding of apoflavodoxin from Azotobacter vinelandii has been followed at the residue level by NMR spectroscopy. NH groups of 21 residues of the protein could be followed in a series of 1H-15N heteronuclear single-quantum coherence spectra recorded at increasing concentrations of...
nmrlearner
Journal club
0
11-18-2010 09:15 PM
NMR Spectroscopy Helps to Study Sam68 Protein Structure - AZoNano.com
NMR Spectroscopy Helps to Study Sam68 Protein Structure - AZoNano.com
<img alt="" height="1" width="1" />
NMR Spectroscopy Helps to Study Sam68 Protein Structure
AZoNano.com
Using NMR spectroscopy, Professor Michael Sattler and his team elucidated the spatial structure of the Qua1 region of Sam68, which is responsible for the ...
and more »
Read here
nmrlearner
Online News
0
09-26-2010 08:07 PM
Bruker Announces 900 MHz NMR Systems Order from the National Institute of ... - Marke
Bruker Announces 900 MHz NMR Systems Order from the National Institute of ... - MarketWatch (press release)
<img alt="" height="1" width="1" />
Bruker Announces 900 MHz NMR Systems Order from the National Institute of ...
MarketWatch (press release)
... thermodynamics and kinetics of protein-protein and protein-nucleic acid interactions at the molecular level requires an NMR system with the highest ...
and more »
Read here
Systems-level Thinking Helps to Address Protein Folding - Institute for Ethics and Emerging Technologies
Linear Mode
