(July 12, 2009) -- Investigators have used nuclear magnetic resonance methods to determine the structure of the largest membrane-spanning protein to date. The group's ability to determine the NMR structure of the bacterial protein diacylglycerol kinase, reported in the journal Science, suggests that similar methods can now be used to study the stru
NMR Structural Biology and Biophysics of Protein:Protein Complexes, National Cancer Institute, Frederick, MD, United States
NMR Structural Biology and Biophysics of Protein:Protein Complexes, National Cancer Institute, Frederick, MD, United States
Postdoctoral positions are available in the Macromolecular NMR section of the Structural Biophysics Laboratory headed by Dr. R. Andrew Byrd at the National Cancer Institute. The Byrd lab combines NMR structural biology & Small Angle Xray Scattering (...
More...
nmrlearner
Job marketplace
0
11-15-2011 11:36 PM
Measurement of protein unfolding/refolding kinetics and structural characterization of hidden intermediates by NMR relaxation dispersion [Biophysics and Computational Biology]
Measurement of protein unfolding/refolding kinetics and structural characterization of hidden intermediates by NMR relaxation dispersion
Meinhold, D. W., Wright, P. E....
Date: 2011-05-31
Detailed understanding of protein function and malfunction hinges on the ability to characterize transiently populated states and the transitions between them. Here, we use 15N, , and 13CO NMR R2 relaxation dispersion to investigate spontaneous unfolding and refolding events of native apomyoglobin. Above pH 5.0, dispersion is dominated by processes involving fluctuations of the F-helix region, which...
nmrlearner
Journal club
0
05-31-2011 11:41 PM
Structural biology: Breaking the protein rules - Nature.com (subscription)
Structural biology: Breaking the protein rules - Nature.com (subscription)
http://nt3.ggpht.com/news/tbn/i9Uh06f_mN2VpM/6.jpg
Nature.com (subscription)
<img alt="" height="1" width="1" />
Structural biology: Breaking the protein rules
Nature.com (subscription)
As is Martin Blackledge, an NMR spectroscopist at the Institute of Structural Biology in Grenoble, France, who compares the excitement now to that surrounding the first crystal protein structures in the 1950s. "Every new case is fascinating at the ...
nmrlearner
Online News
0
03-10-2011 04:29 AM
[NMR paper] Protein NMR recall, precision, and F-measure scores (RPF scores): structure quality a
Protein NMR recall, precision, and F-measure scores (RPF scores): structure quality assessment measures based on information retrieval statistics.
Related Articles Protein NMR recall, precision, and F-measure scores (RPF scores): structure quality assessment measures based on information retrieval statistics.
J Am Chem Soc. 2005 Feb 16;127(6):1665-74
Authors: Huang YJ, Powers R, Montelione GT
One of the most important challenges in modern protein NMR is the development of fast and sensitive structure quality assessment measures that can be...
nmrlearner
Journal club
0
11-24-2010 11:14 PM
[NMR paper] Protein NMR extends into new fields of structural biology.
Protein NMR extends into new fields of structural biology.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein NMR extends into new fields of structural biology.
Curr Opin Chem Biol. 1997 Oct;1(3):359-64
Authors: Cooke RM
Advances in protein NMR have opened new doors for the understanding of macromolecular structure and interactions. Isotope-labelling approaches have extended the size limit for structure determination, the mapping of protein-ligand interactions is...
nmrlearner
Journal club
0
08-22-2010 05:08 PM
NMR in Structural Biology - Alessandro Pintar, Protein Structure and Bioinformatics, ICGEB, Trieste
Nuclear Magnetic Resonance in Structural Biology - Alessandro Pintar, Protein Structure and Bioinformatics, ICGEB, Trieste
Thanks to advancements both in the theory and in the instrumentation, Nuclear Magnetic Resonance (NMR) has widened its use from small organic molecules to oligosaccharides, peptides, proteins, and nucleic acids. Together with X-ray crystallography, NMR is the only technique that can provide structural information at the atomic level. However, applications of NMR are not limited to 3D structure calculation: it can be used to study flexible biomolecules (peptides,...
MCGowan
Educational web pages
0
09-16-2008 01:50 AM
Fundamentals of Protein NMR Spectroscopy (Focus on Structural Biology) - Gordon S. Rule, T. Kevin Hitchens (2005)
Fundamentals of Protein NMR Spectroscopy (Focus on Structural Biology)
By Gordon S. Rule & T. Kevin Hitchens (2005)
Amazon Book description
NMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on 15N/13C-labeled proteins. Beginning with elementary quantum mechanics, a...
JohnW
Books
0
08-19-2008 01:37 PM
NMR RPF: new NMR quality assessment scores
Protein NMR recall, precision, and F-measure scores (RPF scores): structure quality assessment measures based on information retrieval statistics.
Huang YJ, Powers R, Montelione GT.
Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, Northeast Structural Genomics Consortium, and Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5368, USA.
J Am Chem Soc. 2005 Feb 16;127(6):1665-74.
Abstract:
Structural Biology Scores With Protein Snapshot
Linear Mode
