[NMR paper] Stabilization of a flavoprotein for solid-state photo-CIDNP MAS NMR at room temperature by embedding in a glassy sugar matrix
Stabilization of a flavoprotein for solid-state photo-CIDNP MAS NMR at room temperature by embedding in a glassy sugar matrix
Hyperpolarization via the solid-state photochemically induced dynamic nuclear polarization (photo-CIDNP) effect can be detected in frozen solutions of electron transfer proteins generating a radical-pair upon illumination. The effect has been observed in various natural photosynthetic reaction centers and in light-oxygen-voltage (LOV) sensing domains incorporating a flavin mononucleotide (FMN) as chromophore. In LOV domains, where a highly conserved cysteine is...
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06-10-2023 10:27 PM
Dynamics of 4-oxo-TEMPO-d16-(15)N nitroxide-propylene glycol system studied by ESR and ESE in liquid and glassy state in temperature range 10-295K
From The DNP-NMR Blog:
Dynamics of 4-oxo-TEMPO-d16-(15)N nitroxide-propylene glycol system studied by ESR and ESE in liquid and glassy state in temperature range 10-295K
Goslar, J., S.K. Hoffmann, and S. Lijewski, Dynamics of 4-oxo-TEMPO-d16-(15)N nitroxide-propylene glycol system studied by ESR and ESE in liquid and glassy state in temperature range 10-295K. J Magn Reson, 2016. 269: p. 162-75.
http://www.ncbi.nlm.nih.gov/pubmed/27323281
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08-24-2016 04:39 PM
[NMR paper] Characterization of photo-intermediates in the photo-reaction pathways of a bacteriorhodopsin Y185F mutant using in situ photo-irradiation solid-state NMR spectroscopy.
Characterization of photo-intermediates in the photo-reaction pathways of a bacteriorhodopsin Y185F mutant using in situ photo-irradiation solid-state NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Characterization of photo-intermediates in the photo-reaction pathways of a bacteriorhodopsin Y185F mutant using in situ photo-irradiation solid-state NMR spectroscopy.
Photochem Photobiol Sci. 2015 Sep 26;14(9):1694-702
Authors: Oshima K, Shigeta A,...
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06-01-2016 11:08 AM
Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature
From The DNP-NMR Blog:
Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature
This is an incredible article. It shows the temperature dependence of the DNP enhancement over a wide temperature regime. Most importantly it shows that at room temperature still an enhancement of 15-20 can be achieved.
Just a few years ago the common believe was that solid-state MAS-DNP experiments have to be performed at 90 K or below. This article clearly demonstrates that there is still so much room for improvements of DNP. I think the most exciting moments in DNP are...
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03-04-2016 10:23 PM
Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature
From The DNP-NMR Blog:
Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature
Lelli, M., et al., Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature. J Am Chem Soc, 2015. 137(46): p. 14558-61.
http://www.ncbi.nlm.nih.gov/pubmed/26555676
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12-07-2015 06:38 PM
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
From The DNP-NMR Blog:
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
Sakaguchi, S., et al., Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2013. 317(0): p. 679-684.
http://www.sciencedirect.com/science/article/pii/S0168583X13008872
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01-23-2014 01:37 AM
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
From The DNP-NMR Blog:
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
Sakaguchi, S., et al., Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2013(0).
http://www.sciencedirect.com/science/article/pii/S0168583X13008872
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11-21-2013 01:14 AM
Solid-state photo-CIDNP effect observed in phototropin LOV1-C57S by (13)C magic-angle spinning NMR spectroscopy.
Solid-state photo-CIDNP effect observed in phototropin LOV1-C57S by (13)C magic-angle spinning NMR spectroscopy.
Solid-state photo-CIDNP effect observed in phototropin LOV1-C57S by (13)C magic-angle spinning NMR spectroscopy.
J Am Chem Soc. 2010 Nov 10;132(44):15542-3
Authors: Thamarath SS, Heberle J, Hore PJ, Kottke T, Matysik J
Until now, the solid-state photo-CIDNP effect, discovered in 1994 by Zysmilich and McDermott, has been observed selectively in photosynthetic systems. Here we present the first observation of this effect in a...
Stabilization of a flavoprotein for solid-state photo-CIDNP MAS NMR at room temperature by embedding in a glassy ... - ScienceDirect.com
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