Small Angle X-Ray Scattering and the Determination of Protein Shape - Azom.com

		BioNMR > NMR community > Online News
Small Angle X-Ray Scattering and the Determination of Protein Shape - Azom.com

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-20-2013, 06:24 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,714

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Small Angle X-Ray Scattering and the Determination of Protein Shape - Azom.com

Small Angle X-Ray Scattering and the Determination of Protein Shape - Azom.com

Azom.com

Small Angle X-Ray Scattering and the Determination of Protein Shape
Azom.com
The low resolution 3D structure obtained by ab-initio methods is an important additional data to the high resolution structures obtained by protein crystallography and NMR. This article discusses the application of SAXSess mc system from Anton Paar to ...

and more »
Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Understanding Protein Crystallization Growth at the University of ... - Azom.com Understanding Protein Crystallization Growth at the University of ... - Azom.com http://www.bionmr.com//nt2.ggpht.com/news/tbn/YvGbSkpEZpDV7M/6.jpg Azom.com <img alt="" height="1" width="1" /> Understanding Protein Crystallization Growth at the University of ... Azom.com Understanding Protein Crystallization Growth at the University of Leeds using a ... Mass Spectrometers · Nuclear Magnetic Resonance Spectroscopy (NMR) ...	nmrlearner	Online News	0	06-28-2012 07:54 AM
Impact of (15)N R(2)/R(1) Relaxation Restraints on Molecular Size, Shape, and Bond Vector Orientation for NMR Protein Structure Determination with Sparse Distance Restraints. Impact of (15)N R(2)/R(1) Relaxation Restraints on Molecular Size, Shape, and Bond Vector Orientation for NMR Protein Structure Determination with Sparse Distance Restraints. Impact of (15)N R(2)/R(1) Relaxation Restraints on Molecular Size, Shape, and Bond Vector Orientation for NMR Protein Structure Determination with Sparse Distance Restraints. J Am Chem Soc. 2011 Apr 4; Authors: Ryabov Y, Schwieters CD, Clore GM (15)N R(2)/R(1) relaxation data contain information on molecular shape and size as well as on bond vector orientations relative to...	nmrlearner	Journal club	0	04-06-2011 10:54 AM
Impact of 15N R2/R1 Relaxation Restraints on Molecular Size, Shape, and Bond Vector Orientation for NMR Protein Structure Determination with Sparse Distance Restraints Impact of 15N R2/R1 Relaxation Restraints on Molecular Size, Shape, and Bond Vector Orientation for NMR Protein Structure Determination with Sparse Distance Restraints Yaroslav Ryabov, Charles D. Schwieters and G. Marius Clore http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja201020c/aop/images/medium/ja-2011-01020c_0002.gif Journal of the American Chemical Society DOI: 10.1021/ja201020c http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/3J1IyCLkQMQ	nmrlearner	Journal club	0	04-05-2011 10:37 AM
[NMR paper] Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy. Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy. Related Articles Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy. J Am Chem Soc. 2005 Sep 21;127(37):12965-74 Authors: Andronesi OC, Becker S, Seidel K, Heise H, Young HS, Baldus M It is shown that molecular structure and dynamics of a uniformly labeled membrane protein can be studied under magic-angle-spinning conditions. For this purpose, dipolar recoupling experiments...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] HYPER: a hierarchical algorithm for automatic determination of protein dihedral-angle HYPER: a hierarchical algorithm for automatic determination of protein dihedral-angle constraints and stereospecific C beta H2 resonance assignments from NMR data. Related Articles HYPER: a hierarchical algorithm for automatic determination of protein dihedral-angle constraints and stereospecific C beta H2 resonance assignments from NMR data. J Biomol NMR. 1999 Nov;15(3):251-64 Authors: Tejero R, Monleon D, Celda B, Powers R, Montelione GT A new computer program, HYPER, has been developed for automated analysis of protein dihedral angle values...	nmrlearner	Journal club	0	11-18-2010 08:31 PM
NMR and Small Angle Scattering-based structural analysis of protein complexes in solu NMR and Small Angle Scattering-based structural analysis of protein complexes in solution. Related Articles NMR and Small Angle Scattering-based structural analysis of protein complexes in solution. J Struct Biol. 2010 Nov 10; Authors: Madl T, Gabel F, Sattler M Structural analysis of multi-domain protein complexes is a key challenge in current biology and a prerequisite for understanding the molecular basis of essential cellular processes. The use of solution techniques is important for characterizing the quaternary arrangements and dynamics of...	nmrlearner	Journal club	0	11-16-2010 04:13 PM
A structure refinement protocol combining NMR residual dipolar couplings and small angle scattering restraints A structure refinement protocol combining NMR residual dipolar couplings and small angle scattering restraints F. Gabel, B. Simon, M. Nilges, M. Petoukhov, D. Svergun and M. Sattler Journal of Biomolecular NMR; 2008; 41(4); pp 199-208 Abstract: We present the implementation of a target function based on Small Angle Scattering data (Gabel et al. Eur Biophys J 35(4):313–327, 2006) into the Crystallography and NMR Systems (CNS) and demonstrate its utility in NMR structure calculations by simultaneous application of small angle scattering (SAS) and residual dipolar coupling (RDC)...	Abe	Journal club	0	09-21-2008 11:30 PM
Multidomain Protein Structures from NMR & Solution Small-Angle X-ray Scattering http://pubs.acs.org/isubscribe/journals/jacsat/127/i47/figures/ja054342mn00001.gif Refinement of Multidomain Protein Structures by Combination of Solution Small-Angle X-ray Scattering and NMR Data Alexander Grishaev,* Justin Wu, Jill Trewhella, and Ad Bax* Contribution from the Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0520, Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, and Department of Chemistry, University of Utah, Salt Lake City, Utah 84112-0850 J. Am. Chem. Soc.; 2005; 127(47) pp 16621 -...	nmrlearner	Journal club	0	01-12-2006 08:23 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off