[NMR paper] 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
Chem Commun (Camb). 2016 Jul 29;
Authors: Pan BB, Yang F, Ye Y, Wu Q, Li C, Huber T, Su XC
Abstract
Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of...
Watching protein structure at work in living cells using NMR spectroscopy
Watching protein structure at work in living cells using NMR spectroscopy
December 2012
Publication year: 2012
Source:Current Opinion in Chemical Biology, Volume 16, Issues 5–6</br>
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Isotope-assisted multi-dimensional NMR spectroscopy can now be applied to proteins inside living cells. The technique, called in-cell NMR, aims to investigate the structures, interactions and dynamics of proteins under their native conditions, ideally at an atomic resolution. The application has begun with bacterial cells but has now expanded to mammalian cultured cells, such as HeLa...
In-situ measurement of 3-D protein structure inside living eukaryotic cells - Phys.org
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