In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience
In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience
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In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies
SelectScience
Perdeuteration of polypeptides is a useful technique for studying large polypeptides in solution, however this can be difficult to do in vivo. This application note summarizes work using cell-free synthesis methods employing both H2O-based and 2H2O ...
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07-28-2017 08:34 AM
[U. of Ottawa NMR Facility Blog] Chemical Exchange Agents to Simplify NMR Spectra
Chemical Exchange Agents to Simplify NMR Spectra
One can simplify 1H NMR spectra by eliminating exchangeable proton signals. This is most commonly done by adding a drop or two of D2O to the NMR sample. An example of this can be seen in a previous post. The deuterium from the D2O replaces the exchangeable protons (-OH, -NH, -NH2, -COOH) of the sample and their 1H signals disappear. The disadvantage of this technique is the introduction of a strong HDO signal which may overlap with other signals in the spectrum and thereby hinder the interpretation.
An alternative of the "D2O shake"...
Simplify and accelerate protein sample preparation with automated buffer exchange on SelectScience - SelectScience
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