[NMR paper] Time-resolved solid state NMR of biomolecular processes with millisecond time resolution
Time-resolved solid state NMR of biomolecular processes with millisecond time resolution
We review recent efforts to develop and apply an experimental approach to the structural characterization of transient intermediate states in biomolecular processes that involve large changes in molecular conformation or assembly state. This approach depends on solid state nuclear magnetic resonance (ssNMR) measurements that are performed at very low temperatures, typically 25-30 K, with signal enhancements from dynamic nuclear polarization (DNP). This approach also involves novel technology for...
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08-24-2022 02:40 PM
[NMR paper] Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy
Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy
Formation of protein aggregates or fibrils entails the conversion of soluble native protein monomers via multiple molecular states. No spectroscopic techniques have succeeded in capturing the transient molecular-scale events of fibrillation in situ. Here we report residue- and state-specific real-time monitoring of the fibrillation of amyotrophic lateral sclerosis-related SOD1 by rheology NMR (Rheo-NMR) spectroscopy. Under moderately denaturing conditions, where NMR signals of folded...
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07-08-2021 11:44 AM
Time-resolved protein activation by proximal decaging in living systems - Nature.com
Time-resolved protein activation by proximal decaging in living systems - Nature.com
Time-resolved protein activation by proximal decaging in living systems Nature.comA universal gain-of-function approach for selective and temporal control of protein activity in living systems is crucial to understanding dynamic cellular ...
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05-09-2019 05:55 AM
[NMR paper] Time-resolved in situ MAS NMR monitoring of the nucleation and growth of zeolite BEA catalysts under hydrothermal conditions
Time-resolved in situ MAS NMR monitoring of the nucleation and growth of zeolite BEA catalysts under hydrothermal conditions
Time-resolved 13C, 23Na, 27Al and 29Si MAS NMR has been applied in situ for monitoring the hydrothermal synthesis of zeolite BEA. Isotopic labelling with 29Si and 13C isotopes has been used to follow the fate of silicious species and structure directing agent ((13CH3-CH2)4NOH). Two mechanistic pathways, namely, solution mediated and solid-solid hydrogel rearrangement have been distinguished for two synthesis procedures studied. The mechanisms of structure directing...
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09-21-2017 02:38 AM
Journal Highlight: 'NMR Crystallization': in-situ NMR techniques for time-resolved monitoring of crystallization processes
Journal Highlight: 'NMR Crystallization': in-situ NMR techniques for time-resolved monitoring of crystallization processes
http://www.spectroscopynow.com/common/images/thumbnails/15b331a8faa.jpgAn overview of the range of NMR strategies currently available for in-situ studies of crystallization processes is presented, with examples of applications that highlight their potential to deepen our understanding of crystallization phenomena.
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04-03-2017 05:37 PM
[NMR paper] Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts.
Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts.
Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts.
Nat Protoc. 2013 Jun 27;8(7):1416-1432
Authors: Theillet FX, Rose HM, Liokatis S, Binolfi A, Thongwichian R, Stuiver M, Selenko P
Abstract
We outline NMR protocols for site-specific mapping and time-resolved monitoring of...
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06-29-2013 11:49 AM
Site-Specific Mapping and Time-Resolved Monitoring of Lysine Methylation by High-Resolution NMR Spectroscopy
Site-Specific Mapping and Time-Resolved Monitoring of Lysine Methylation by High-Resolution NMR Spectroscopy
Franc?ois-Xavier Theillet, Stamatios Liokatis, Jan Oliver Jost, Beata Bekei, Honor May Rose, Andres Binolfi, Dirk Schwarzer and Philipp Selenko
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja301895f/aop/images/medium/ja-2012-01895f_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja301895f
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/tV2dPnMa4Qc
Residue-resolved monitoring of protein hyperpolarization at sub-second time resolution - Nature.com
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