[NMR paper] Direct Observation of Membrane-Associated H-Ras in the Native Cellular Environment by In-Cell 19F-NMR Spectroscopy
Direct Observation of Membrane-Associated H-Ras in the Native Cellular Environment by In-Cell 19F-NMR Spectroscopy
Ras acts as a molecular switch to control intracellular signaling on the plasma membrane (PM). Elucidating how Ras associates with PM in the native cellular environment is crucial for understanding its control mechanism. Here, we used in-cell nuclear magnetic resonance (NMR) spectroscopy combined with site-specific ^(19)F-labeling to explore the membrane-associated states of H-Ras in living cells. The site-specific incorporation of p-trifluoromethoxyphenylalanine (OCF(3)Phe)...
[NMR paper] Protein environment affects the water-tryptophan binding mode. MD, QM/MM, and NMR studies of engrailed homeodomain mutants.
Protein environment affects the water-tryptophan binding mode. MD, QM/MM, and NMR studies of engrailed homeodomain mutants.
Related Articles Protein environment affects the water-tryptophan binding mode. MD, QM/MM, and NMR studies of engrailed homeodomain mutants.
Phys Chem Chem Phys. 2018 Apr 26;:
Authors: Špa?ková N, Trošanová Z, Šebesta F, Jansen S, Burda JV, Srb P, Zachrdla M, Žídek L, Kozelka J
Abstract
Water molecules can interact with aromatic moieties using either their O-H bonds or their lone-pairs of electrons. In...
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Researchers observe the switching of Ras protein in detail - Phys.Org
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Phys.Org
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Researchers observe the switching of Ras protein in detail
Phys.Org
Ras proteins are molecular switches that decide if and when cells divide inside our bodies. An impairment of their function may result in the formation of a tumour. The process of switching the proteins on and off has been observed in detail by a ...
Researchers observe the switching of Ras protein in detail - Phys.Org
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Difference between Extra- and IntracellularT1Values of Carboxylic Acids Affects the Quantitative Analysis of Cellular Kinetics by Hyperpolarized NMR
From The DNP-NMR Blog:
Difference between Extra- and IntracellularT1Values of Carboxylic Acids Affects the Quantitative Analysis of Cellular Kinetics by Hyperpolarized NMR
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Karlsson, M., et al., Difference between Extra- and IntracellularT1Values of Carboxylic Acids Affects the Quantitative Analysis of Cellular Kinetics by Hyperpolarized NMR. Angewandte Chemie, 2016. 128(43): p. 13765-13768.
http://dx.doi.org/10.1002/ange.201607535
COMPASS method points researchers to protein structures - Phys.Org
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Phys.Org
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COMPASS method points researchers to protein structures
Phys.Org
Graduate student Joseph Courtney and chemistry professor Chad Rienstra developed a method to quickly and reliably determine a proteins intricately folded structure Credit: L. Brian Stauffer. Searching for the precise, complexly folded three-dimensional ...
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COMPASS method points researchers to protein structures - Phys.Org
Researchers investigate how the cellular environment affects protein conformational dynamics - Phys.org
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