[NMR paper] Rapid Protein-Ligand Affinity Determination by Photoinduced Hyperpolarized NMR
Rapid Protein-Ligand Affinity Determination by Photoinduced Hyperpolarized NMR
The binding affinity determination of protein-ligand complexes is a cornerstone of drug design. State-of-the-art techniques are limited by lengthy and expensive processes. Building upon our recently introduced novel screening method utilizing photochemically induced dynamic nuclear polarization (photo-CIDNP) NMR, we provide the methodological framework to determine binding affinities within 5-15 min using 0.1 mg of protein. The accuracy of our method is demonstrated for the affinity constants of...
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Rapid Protein–Ligand Affinity Determination by Photoinduced Hyperpolarized NMR - American Chemical Society
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