Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review

		BioNMR > NMR community > Online News
Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

01-07-2019, 05:49 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review

Quality assessment of biologics: higher order structure analysis using NMR European Pharmaceutical ReviewThe webinar will describe how 2D NMR can offer protein biochemists unprecedented analytical precision. By assigning just one spectrum, users can obtain a ...

Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review
More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Application of 2D-NMR with room temperature NMR probes for the assessment of the higher order structure of filgrastim. Application of 2D-NMR with room temperature NMR probes for the assessment of the higher order structure of filgrastim. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Application of 2D-NMR with room temperature NMR probes for the assessment of the higher order structure of filgrastim. J Pharm Biomed Anal. 2017 Jul 15;141:229-233 Authors: Brinson RG, Ghasriani H, Hodgson DJ, Adams KM, McEwen I, Freedberg DI, Chen K, Keire DA, Aubin Y, Marino JP ...	nmrlearner	Journal club	0	01-26-2018 12:08 PM
[NMR paper] Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure. Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure. Related Articles Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure. J Pharm Biomed Anal. 2016 Jun 7;128:398-407 Authors: Chen K, Long DS, Lute SC, Levy MJ, Brorson KA, Keire DA Abstract Monoclonal antibody (mAb) drugs constitute the largest class of protein therapeutics currently on the market. Correctly folded protein higher order structure...	nmrlearner	Journal club	0	06-28-2016 06:25 PM
[NMR paper] On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods. On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods. Related Articles On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods. Anal Chem. 2015 Apr 30; Authors: Poppe L, Jordan JB, Rogers G, Schnier PD Abstract An important aspect in the analytical characterization of protein therapeutics is the comprehensive characterization of higher...	nmrlearner	Journal club	0	05-02-2015 09:41 PM
Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy From The DNP-NMR Blog: Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy Debelouchina, G.T., et al., Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy. J Am Chem Soc, 2013. 135(51): p. 19237-47. http://www.ncbi.nlm.nih.gov/pubmed/24304221	nmrlearner	News from NMR blogs	0	01-27-2014 09:59 PM
[NMR paper] Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy. Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy. Related Articles Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy. J Am Chem Soc. 2013 Dec 4; Authors: Debelouchina GT, Bayro MJ, Fitzpatrick AW, Ladizhansky V, Colvin MT, Caporini MA, Jaroniec CP, Bajaj VS, Rosay MM, Macphee CE, Vendruscolo M, Maas WE, Dobson CM, Griffin RG Abstract Protein magic angle spinning (MAS) NMR spectroscopy has generated structural models of several amyloid fibril systems, thus providing valuable information regarding the...	nmrlearner	Journal club	0	12-07-2013 01:00 PM
Journal Highlight: Assessment of higher order structure comparability in ... - spectroscopyNOW.com <img alt="" height="1" width="1" /> Journal Highlight: Assessment of higher order structure comparability in ... spectroscopyNOW.com Abstract: In this work, we applied nuclear magnetic resonance (NMR) spectroscopy to rapidly assess higher order structure (HOS) comparability in protein samples. Using a variation of the NMR fingerprinting approach described by Panjwani et al. Journal Highlight: Assessment of higher order structure comparability in ... - spectroscopyNOW.com More...	nmrlearner	Online News	0	06-03-2013 04:21 PM
Journal Highlight: Assessment of higher order structure comparability in therapeutic proteins using nuclear magnetic resonance spectroscopy Journal Highlight: Assessment of higher order structure comparability in therapeutic proteins using nuclear magnetic resonance spectroscopy http://www.spectroscopynow.com/common/images/thumbnails/13ef9b3d882.jpgNMR spectroscopy using a fingerprinting approach has been used to rapidly assess higher order structure comparability in three nonglycosylated proteins spanning a molecular weight range of 6.5–67 kDa. Read the rest at Spectroscopynow.com	nmrlearner	General	0	06-03-2013 04:21 PM
Affymetrix launches NG Class detergents for better protein analysis - Pharmaceutical Business Review <img alt="" height="1" width="1" /> Affymetrix launches NG Class detergents for better protein analysis Pharmaceutical Business Review Affymetrix chief commercial officer Andy Last said the new NG Class Detergents give biologists enhanced tools to solve the structure of membrane proteins using advanced techniques, such as nuclear magnetic resonance and X-ray diffraction. ... and more » Affymetrix launches NG Class detergents for better protein analysis - Pharmaceutical Business Review More...	nmrlearner	Online News	0	02-15-2011 09:29 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off