[NMR paper] 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
Chem Commun (Camb). 2016 Jul 29;
Authors: Pan BB, Yang F, Ye Y, Wu Q, Li C, Huber T, Su XC
Abstract
Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of...
[NMR paper] Protein dynamics in living cells studied by in-cell NMR spectroscopy.
Protein dynamics in living cells studied by in-cell NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein dynamics in living cells studied by in-cell NMR spectroscopy.
FEBS Lett. 2013 Jan 11;
Authors: Li C, Liu M
Abstract
Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques...