[NMR paper] Protein methylation characterization using NMR without isotopic labeling
Protein methylation characterization using NMR without isotopic labeling
Protein methylation is crucial in epigenetics, and targeting the involved methyltransferases shows great potential for therapeutic intervention with several inhibitors in clinical trials for oncology indications. Therefore, characterization of protein methylation is essential for understanding the methyltransferase function and discovering chemical inhibitors and antagonists. While NMR has been used to measure methylation rates, isotopic labeling of protein or methyl donors can be costly and...
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Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation
Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation
Abstract
Segmental isotopic labeling can facilitate NMR studies of large proteins, multi-domain proteins, and proteins with repetitive sequences by alleviating NMR signal overlaps. Segmental isotopic labeling also allows us to investigate an individual domain in the context of a full-length protein by NMR. Several established methods are available for segmental isotopic labeling such as intein-mediated ligation, but each has specific requirements and limitations. Here,...
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03-13-2018 07:39 PM
NMR characterization of a 72 kDa transcription factor using differential isotopic labeling
NMR characterization of a 72 kDa transcription factor using differential isotopic labeling
Abstract
NF-?B is a major transcription factor that mediates a number of cellular signaling pathways. Crystal structure analysis gives an incomplete picture of the behavior of the protein, particularly in the free state; free monomers or dimers of NF-?B have never been crystallized. NMR analysis gives insights into the structure and dynamics of the protein in solution, but a necessary first step is the assignment of resonances. The size of the heterodimer of the Rel homology regions of the NF-?B...
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12-28-2015 12:26 AM
[NMR paper] NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling.
NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling.
Related Articles NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling.
Protein Sci. 2015 Dec 8;
Authors: Mukherjee SP, Borin B, Quintas PO, Dyson HJ
Abstract
NF-?B is a major transcription factor that mediates a number of cellular signaling pathways. Crystal structure analysis gives an incomplete picture of the behavior of the protein, particularly in the free state; free monomers or dimers of...
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12-10-2015 05:49 PM
NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling
NMR Characterization of a 72 kDa transcription factor using differential isotopic labeling
Abstract
NF-?B is a major transcription factor that mediates a number of cellular signaling pathways. Crystal structure analysis gives an incomplete picture of the behavior of the protein, particularly in the free state; free monomers or dimers of NF-?B have never been crystallized. NMR analysis gives insights into the structure and dynamics of the protein in solution, but a necessary first step is the assignment of resonances. The size of the heterodimer of the Rel homology regions of the NF-?B...
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12-08-2015 08:28 PM
The Elusive Structure of Pd2(dba)3. Examination by Isotopic Labeling, NMR Spectroscopy, and X-rayDiffraction Analysis: Synthesis and Characterization of Pd2(dba-Z)3 Complexes
The Elusive Structure of Pd2(dba)3. Examination by Isotopic Labeling, NMR Spectroscopy, and X-rayDiffraction Analysis: Synthesis and Characterization of Pd2(dba-Z)3 Complexes
Anant R. Kapdi, Adrian C. Whitwood, David C. Williamson, Jason M. Lynam, Michael J. Burns, Thomas J. Williams, Alan J. Reay, Jordan Holmes and Ian J. S. Fairlamb
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja403259c/aop/images/medium/ja-2013-03259c_0018.gif
Journal of the American Chemical Society
DOI: 10.1021/ja403259c...
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05-24-2013 08:54 AM
[NMR paper] Recent applications of isotopic labeling for protein NMR in drug discovery.
Recent applications of isotopic labeling for protein NMR in drug discovery.
Related Articles Recent applications of isotopic labeling for protein NMR in drug discovery.
Expert Opin Drug Discov. 2013 Mar 12;
Authors: Hiroaki H
Abstract
Introduction: Nuclear magnetic resonance (NMR) applications in drug discovery are classified into two categories: ligand-based methods and protein-based methods. The latter is based on the observation of the (1)H-(15)N HSQC spectra of a protein with and without lead compounds. However, in order to take this...
Protein methylation characterization using NMR without isotopic labeling - ScienceDirect
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