Protein Characterisation Techniques: How to Characterise a Protein - Labmate Online

		BioNMR > NMR community > Online News
Protein Characterisation Techniques: How to Characterise a Protein - Labmate Online

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-20-2021, 11:06 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Protein Characterisation Techniques: How to Characterise a Protein - Labmate Online

Protein Characterisation Techniques: How to Characterise a Protein - Labmate Online

Protein Characterisation Techniques: How to Characterise a Protein Labmate Online Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Deep characterisation and quantitative analysis of proteins and post-translational modifications - How TIMS has extended the capabilities of MS and shown considerable potential for improving PTM identification - Labmate Online Deep characterisation and quantitative analysis of proteins and post-translational modifications - How TIMS has extended the capabilities of MS and shown considerable potential for improving PTM identification - Labmate Online Deep characterisation and quantitative analysis of proteins and post-translational modifications - How TIMS has extended the capabilities of MS and shown considerable potential for improving PTM identification Labmate Online Read here	nmrlearner	Online News	0	08-18-2021 11:11 AM
Deep characterisation and quantitative analysis of proteins and post-translational modifications How TIMS has extended the capabilities of MS and shown considerable potential for improving PTM identification and critical understandi - Labmate Online Deep characterisation and quantitative analysis of proteins and post-translational modifications How TIMS has extended the capabilities of MS and shown considerable potential for improving PTM identification and critical understandi - Labmate Online Deep characterisation and quantitative analysis of proteins and post-translational modifications How TIMS has extended the capabilities of MS and shown considerable potential for improving PTM identification and critical understandi Labmate Online Read here	nmrlearner	Online News	0	07-09-2021 04:48 PM
[NMR paper] Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR. Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR. Related Articles Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR. Biology (Basel). 2020 Nov 12;9(11): Authors: Yeh V, Goode A, Bonev BB Abstract Biological membranes define the interface of life and its basic unit, the cell. Membrane proteins play key roles in membrane functions, yet their structure and mechanisms remain poorly understood. Breakthroughs in crystallography and electron...	nmrlearner	Journal club	0	11-19-2020 06:30 AM
[NMR paper] Sequential Protein Expression and Capsid Assembly In Cell: Towards the Study of Multi-Protein Viral Capsids Using Solid-State NMR Techniques. Sequential Protein Expression and Capsid Assembly In Cell: Towards the Study of Multi-Protein Viral Capsids Using Solid-State NMR Techniques. Related Articles Sequential Protein Expression and Capsid Assembly In Cell: Towards the Study of Multi-Protein Viral Capsids Using Solid-State NMR Techniques. Biochemistry. 2018 Feb 21;: Authors: Alphonse S, Itin B, Khayat R, Ghose R Abstract While solid-state NMR (ssNMR) has emerged as a powerful technique to study viral capsids, current studies are limited to capsids formed from single...	nmrlearner	Journal club	0	02-23-2018 03:44 AM
FEI and the National Center for Protein Sciences Beijing Launch Training ... - Labmate Online FEI and the National Center for Protein Sciences Beijing Launch Training ... - Labmate Online <img alt="" height="1" width="1"> FEI and the National Center for Protein Sciences Beijing Launch Training ... Labmate Online The adopted workflow solution opens the door to understanding the structure and function of whole classes of proteins and protein complexes that are simply beyond the capabilities of NMR and XRD alone. An integrative approach that incorporates cryo-EM ... Read here	nmrlearner	Online News	0	03-25-2015 10:15 AM
What Questions Can I Answer with Nuclear Magnetic Resonance? - Labmate Online <img alt="" height="1" width="1" /> What Questions Can I Answer with Nuclear Magnetic Resonance? Labmate Online Nuclear magnetic resonance (NMR) is a physical process that occurs in some nuclei when they are subjected to electromagnetic fields. The process is used in chemistry as NMR spectroscopy and in medicine as magnetic resonance imaging (MRI). Using NMR to study protein motion: an interview with Fabien Ferrage, Ã?cole ...News-Medical.net all 2 news articles » What Questions Can I Answer with Nuclear Magnetic Resonance? - Labmate Online More...	nmrlearner	Online News	0	08-19-2014 11:21 AM
Developing NMR Methods to Analyse Large Proteins - Labmate Online Developing NMR Methods to Analyse Large Proteins - Labmate Online <img alt="" height="1" width="1" /> Developing NMR Methods to Analyse Large Proteins Labmate Online The award will total nearly $373000 over three years to help the lab develop fast and efficient assignment methods for large proteins by NMR and make these methods accessible to the structural biology community. â??NMR spectroscopy has still a tremendous ... Read here	nmrlearner	Online News	0	12-14-2011 07:14 PM
[NMR paper] NMR techniques for identifying the interface of a larger protein-protein complex: cro NMR techniques for identifying the interface of a larger protein-protein complex: cross-saturation and transferred cross-saturation experiments. Related Articles NMR techniques for identifying the interface of a larger protein-protein complex: cross-saturation and transferred cross-saturation experiments. Methods Enzymol. 2005;394:483-506 Authors: Shimada I NMR provides detailed structural information for protein complexes with molecular weights up to 30 kDa. However, it is difficult to obtain such information on larger proteins using NMR. To...	nmrlearner	Journal club	0	11-24-2010 11:14 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off