Probing the energy barriers and stages of membrane protein unfolding using solid-state NMR spectroscopy - Science

		BioNMR > NMR community > Online News
Probing the energy barriers and stages of membrane protein unfolding using solid-state NMR spectroscopy - Science

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-18-2024, 04:40 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Probing the energy barriers and stages of membrane protein unfolding using solid-state NMR spectroscopy - Science

Probing the energy barriers and stages of membrane protein unfolding using solid-state NMR spectroscopy - Science

Probing the energy barriers and stages of membrane protein unfolding using solid-state NMR spectroscopy Science Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solid-state NMR structure determination of a membrane protein in E. coli cellular inner membrane - Science Solid-state NMR structure determination of a membrane protein in E. coli cellular inner membrane - Science Solid-state NMR structure determination of a membrane protein in E. coli cellular inner membrane Science Read here	nmrlearner	Online News	0	07-24-2024 08:25 AM
Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway - Nature.com Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway - Nature.com Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway Nature.com Read here	nmrlearner	Online News	0	07-24-2024 08:25 AM
[NMR paper] Probing the energy barriers and stages of membrane protein unfolding using solid-state NMR spectroscopy Probing the energy barriers and stages of membrane protein unfolding using solid-state NMR spectroscopy Understanding how the amino acid sequence dictates protein structure and defines its stability is a fundamental problem in molecular biology. It is especially challenging for membrane proteins that reside in the complex environment of a lipid bilayer. Here, we obtain an atomic-level picture of the thermally induced unfolding of a membrane-embedded ?-helical protein, human aquaporin 1, using solid-state nuclear magnetic resonance spectroscopy. Our data reveal the hierarchical two-step...	nmrlearner	Journal club	0	05-17-2024 11:00 PM
Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway - Nature.com Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway - Nature.com Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway Nature.com Read here	nmrlearner	Online News	0	12-28-2023 10:50 PM
[NMR paper] Probing the Conformationally Excited States of Membrane Proteins via (1)H-detected MAS Solid-State NMR Spectroscopy. Probing the Conformationally Excited States of Membrane Proteins via (1)H-detected MAS Solid-State NMR Spectroscopy. Probing the Conformationally Excited States of Membrane Proteins via (1)H-detected MAS Solid-State NMR Spectroscopy. J Phys Chem B. 2017 Apr 13;: Authors: Gopinath T, Nelson SE, Soller KJ, Veglia G Abstract Proteins exist in ensembles of conformational states that interconvert on various motional time scales. High-energy states of proteins, often referred to as conformationally excited states, are sparsely...	nmrlearner	Journal club	0	04-14-2017 10:27 AM
Nmr Investigation of Energy Barriers for Hydrogen-Bond Breakage of Protein Side-Chain NH3+ Groups Nmr Investigation of Energy Barriers for Hydrogen-Bond Breakage of Protein Side-Chain NH3+ Groups Publication date: 27 January 2015 Source:Biophysical Journal, Volume 108, Issue 2, Supplement 1</br> Author(s): Levani Zandarashvili , Junji Iwahara</br> </br></br> </br></br> More...	nmrlearner	Journal club	0	01-28-2015 05:28 PM
[NMR paper] Probing Membrane Protein Structure Using Water Polarization Transfer Solid-State NMR Probing Membrane Protein Structure Using Water Polarization Transfer Solid-State NMR Publication date: Available online 25 August 2014 Source:Journal of Magnetic Resonance</br> Author(s): Jonathan K. Williams , Mei Hong</br> Water plays an essential role in the structure and function of proteins, lipid membranes and other biological macromolecules. Solid-state NMR heteronuclear-detected 1H polarization transfer from water to biomolecules is a versatile approach for studying water-protein, water-membrane, and water-carbohydrate interactions in biology. We review...	nmrlearner	Journal club	0	08-26-2014 01:14 AM
Probing the Membrane Bound KCNE1 Protein with Solid State NMR Spectroscopy Probing the Membrane Bound KCNE1 Protein with Solid State NMR Spectroscopy 29 January 2013 Publication year: 2013 Source:Biophysical Journal, Volume 104, Issue 2, Supplement 1</br> </br> </br> </br></br>	nmrlearner	Journal club	0	02-03-2013 10:13 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off