Pakistani scholar gets Doctorate degree from Oxford Daily Times
He applied Nuclear Magnetic Resonance (NMR) techniques to study the mode of drug binding to BBOX and bacterial potassium ion efflux (Kef) proteins. Human BBOX is currently a drug target for the treatment of ischemic heart diseases, while scientists at ...
Pakistani scholar gets Doctorate degree from Oxford - Daily Times More...
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[Question from NMRWiki Q&A forum] 15N 90 degree hard pulse calibration
15N 90 degree hard pulse calibration
Greetings, I want to ask for some advice in matter of 15N hard pulse calibration on Bruker machines. I'm working on Avance DRX 500 with 5mm TBI probe. This calibration is easy to made if 15N urea is avaible, but I don't have opportunity to use this compound. I have in my stable horses as follows:
methylamine hydrochloride (99% 15N) ammonium chloride (99,9% 15N) aniline (15N >99%) sodium nitrite (99% 15N)
I tried to use first two salts in DMSO-d6. Unfortunately I couldn't obtain proton spectrum with multiplet structure that is needed for pulse...
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08-19-2014 11:21 AM
[NMR paper] NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies.
NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies.
Bioorg Med Chem. 2013 Oct 1;21(19):6069-76
Authors: Hanashima S, Sato C, Tanaka H, Takahashi T, Kitajima K, Yamaguchi Y
Abstract
Oligo/polysialic acids consisting...
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04-08-2014 08:02 PM
Optimal degree of protonation for 1H detection of aliphatic sites in randomly deuterated proteins as a function of the MAS frequency
Optimal degree of protonation for 1H detection of aliphatic sites in randomly deuterated proteins as a function of the MAS frequency
Abstract The 1H dipolar network, which is the major obstacle for applying proton detection in the solid-state, can be reduced by deuteration, employing the RAP (Reduced Adjoining Protonation) labeling scheme, which yields random protonation at non-exchangeable sites. We present here a systematic study on the optimal degree of random sidechain protonation in RAP samples as a function of the MAS (magic angle spinning) frequency. In particular, we compare 1H...
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08-25-2012 08:56 AM
[U. of Ottawa NMR Facility Blog] Probe Tuning and 90 Degree Pulses
Probe Tuning and 90 Degree Pulses
In order to get meaningful results from multiple-pulse NMR pulse sequences, it is essential that the 90° and 180° pulses are calibrated at the power levels used in the sequences (see this post for example). The calibrations are usually done on a standard sample in a well tuned and matched probe. The calibrations are typically stored in a file which is called up when setting up particular NMR experiments. It is important to know that these calibrations are correct for the particular sample of interest only when the probe is well tuned and matched. For...
nmrlearner
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09-16-2011 10:02 PM
Scholar/Scientist
Scholar/Scientist
Position Title: Scholar/Scientist (CHM), Solid State Nuclear Magnetic Resonance (NMR) Spectroscopist
Appointment: 2 years renewable upon mutual agreement
Department: Chemistry and Biochemistry, Florida State University
Salary Range: $50,000 to negotiable
Qualifications: Ph.D. in chemistry with specialized training and experience in solid state NMR spectroscopy.
Requirements:
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09-27-2010 08:59 PM
[U. of Ottawa NMR Facility Blog] Fast 90 Degree Pulse Determination
Fast 90 Degree Pulse Determination
Almost all NMR measurements rely on the correct calibration of 90° pulses. This is traditionally done by collecting a series of spectra as a function of pulse duration, finding a null for the 180° or 360° pulse and calculating the 90° pulse by simple division by 2 or 4 in the case of the 180° and 360° nulls, respectively. This determination, although trivial, can be very time consuming. Wu and Otting* have presented a much faster method of determining a 90° pulse based on measuring the nutation of a magnetization vector directly. Continuous nutation is...