[NMR paper] NMR-Based Analysis of Nanobodies to SARS-CoV-2 Nsp9 Reveals a Possible Antiviral Strategy Against COVID-19
NMR-Based Analysis of Nanobodies to SARS-CoV-2 Nsp9 Reveals a Possible Antiviral Strategy Against COVID-19
Following the entry into the host cell, SARS-CoV-2 replication is mediated by the replication transcription complex (RTC) assembled through a number of nonstructural proteins (Nsps). A monomeric form of Nsp9 is particularly important for RTC assembly and function. In the present study, 136 unique nanobodies targeting Nsp9 are generated. Several nanobodies belonging to different B-cell lineages are expressed, purified, and characterized. Results from immunoassays applied to purified...
Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach
Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach
Abstract
The insertase BamA is an essential protein of the bacterial outer membrane. Its 16-stranded transmembrane β-barrel contains a lateral gate as a key functional element. This gate is formed by the C-terminal half of the last β-strand. The BamA barrel was previously found to sample different conformations in aqueous solution, as well as different gate-open, gate-closed, and collapsed conformations in...
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NMR structure and localization of a large fragment of the SARS-CoV fusion protein: Implications in viral cell fusion
NMR structure and localization of a large fragment of the SARS-CoV fusion protein: Implications in viral cell fusion
Publication date: February 2018
Source:Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1860, Issue 2</br>
Author(s): Mukesh Mahajan, Deepak Chatterjee, Kannaian Bhuvaneswari, Shubhadra Pillay, Surajit Bhattacharjya</br>
The lethal Coronaviruses (CoVs), Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) and most recently Middle East Respiratory Syndrome Coronavirus, (MERS-CoV) are serious human health hazard. A...
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11-23-2017 08:38 AM
[NMR paper] NMR structure and localization of a large fragment of the SARS-CoV fusion protein: Implications in viral cell fusion.
NMR structure and localization of a large fragment of the SARS-CoV fusion protein: Implications in viral cell fusion.
NMR structure and localization of a large fragment of the SARS-CoV fusion protein: Implications in viral cell fusion.
Biochim Biophys Acta. 2017 Oct 05;:
Authors: Mahajan M, Chatterjee D, Bhuvaneswari K, Pillay S, Bhattacharjya S
Abstract
The lethal Coronaviruses (CoVs), Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) and most recently Middle East Respiratory Syndrome Coronavirus, (MERS-CoV)...
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10-11-2017 10:37 AM
Researchers discover protein dynamics help regulate cell division - South Bend Tribune
Researchers discover protein dynamics help regulate cell division - South Bend Tribune
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Researchers discover protein dynamics help regulate cell division
South Bend Tribune
That molecular basis for the cross-protein communication was unclear, until now. Using nuclear magnetic resonance (NMR) spectroscopy, the researchers discovered that Pin1 undergoes different changes in dynamics when it binds different shapes. ...
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NYU Abu Dhabi researchers discover new nanobodies that can disable a key SARS-CoV-2 structural protein and disrupt viral replication - ZAWYA
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