Novel use of EPR spectroscopy to study in vivo protein structure - News-Medical.net

		BioNMR > NMR community > Online News
Novel use of EPR spectroscopy to study in vivo protein structure - News-Medical.net

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-16-2016, 12:13 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Novel use of EPR spectroscopy to study in vivo protein structure - News-Medical.net

Novel use of EPR spectroscopy to study in vivo protein structure - News-Medical.net

News-Medical.net

Novel use of EPR spectroscopy to study in vivo protein structure
News-Medical.net
More recently, increases in the sensitivity of NMR and the use of isotope labelling have enabled determinations of the atomic level structure and dynamics of proteins to be determined within living cells1. NMR has been used to determine the structure ...

Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Study reveals molecular function of protein essential for replication of H5N1 ... - News-Medical.net Study reveals molecular function of protein essential for replication of H5N1 ... - News-Medical.net http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcQqQVkN2sOlkhvYowf7yXaO0RlhCYQZ_9zOSx7m7tG2kLZe9aCik1IvkvJcZVU-_BYhJS__lMZX News-Medical.net <img alt="" height="1" width="1"> Study reveals molecular function of protein essential for replication of H5N1 ... News-Medical.net An international collaboration of scientists from the CEA, CNRS, University Joseph Fourier, the EMBL and the ILL has revealed the molecular function of a protein essential for replication of H5N1 influenza...	nmrlearner	Online News	0	12-10-2015 05:49 PM
Researchers use magic-angle-spinning NMR to unveil structure of CAP-Gly protein - News-Medical.net http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcTyM1uXTIaIw5q1INc3zkifPECmN3ZbTF8KFbDsA_fbY3BMFAuOMi8jvzcjz3gft3z24W0TdB4a News-Medical.net <img alt="" height="1" width="1"> Researchers use magic-angle-spinning NMR to unveil structure of CAP-Gly protein News-Medical.net The research team used magic-angle-spinning nuclear magnetic resonance spectrometry (NMR) in the Department of Chemistry and Biochemistry at UD to unveil the structure of the CAP-Gly protein assembled on polymerized microtubules. The CAP-Gly ... Research team finds that protein motions regulate HIV...	nmrlearner	Online News	0	11-26-2015 12:22 PM
Using NMR to study protein motion: an interview with Fabien Ferrage, Ã?cole ... - News-Medical.net Using NMR to study protein motion: an interview with Fabien Ferrage, Ã?cole ... - News-Medical.net <img alt="" height="1" width="1" /> Using NMR to study protein motion: an interview with Fabien Ferrage, Ã?cole ... News-Medical.net We have first applied the new technique to a model protein ubiquitin, which has been studied by NMR for 20 to 25 years and been very well characterized by various methods, including its structure and motions. What we have seen using this apparatus to ... and more » Read here	nmrlearner	Online News	0	08-19-2014 11:21 AM
Study could lead to better understanding of how Notch protein directs healthy ... - News-Medical.net Study could lead to better understanding of how Notch protein directs healthy ... - News-Medical.net <img alt="" height="1" width="1" /> Study could lead to better understanding of how Notch protein directs healthy ... News-Medical.net Johns Hopkins researchers report they have figured out how the aptly named protein Botch blocks the signaling protein called Notch, which helps regulate development. In a report on the discovery, to appear online April 24 in the journal Cell Reports ... Read here	nmrlearner	Online News	0	05-25-2014 06:31 PM
In vivo Imaging Fluorescent Proteins Expressed from DNA Vaccine Constructs - News-Medical.net In vivo Imaging Fluorescent Proteins Expressed from DNA Vaccine Constructs - News-Medical.net <img alt="" height="1" width="1" /> In vivo Imaging Fluorescent Proteins Expressed from DNA Vaccine Constructs News-Medical.net Nevertheless, most of the clinical trials that reported the use of these proteins have used recombinant viruses; injected cells expressing the proteins; or used recombinant protein in an artificial mouse. This article examines the technique used to ... Read here	nmrlearner	Online News	0	12-17-2013 01:46 PM
Scientists determine structure of Ltn1 protein - News-Medical.net Scientists determine structure of Ltn1 protein - News-Medical.net <img alt="" height="1" width="1" /> Scientists determine structure of Ltn1 protein News-Medical.net Ltn1 was deemed too large for its structure to be determined by current nuclear magnetic resonance (NMR) technology, and, as the scientists know now, too flexible to allow the highly regular crystalline packing needed by X-ray crystallographers. "It's ... Read here	nmrlearner	Online News	0	01-17-2013 02:09 PM
Researchers elucidate Qua1 spatial structure region of Sam68 protein - News-Medical.n Researchers elucidate Qua1 spatial structure region of Sam68 protein - News-Medical.net <img alt="" height="1" width="1" /> Researchers elucidate Qua1 spatial structure region of Sam68 protein News-Medical.net Using NMR spectroscopy, Professor Michael Sattler and his team elucidated the spatial structure of the Qua1 region of Sam68, which is responsible for the ... and more » Read here	nmrlearner	Online News	0	09-13-2010 03:33 PM
Structure of key protein for cellular signal transduction elucidated - News-Medical.n Structure of key protein for cellular signal transduction elucidated - News-Medical.net <img alt="" height="1" width="1" /> Structure of key protein for cellular signal transduction elucidated News-Medical.net Using NMR spectroscopy, Professor Michael Sattler and his team elucidated the spatial structure of the Qua1 region of Sam68, which is responsible for the ... and more » Read here	nmrlearner	Online News	0	09-10-2010 12:58 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off