[NMR paper] DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular milieu
DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular milieu
With the sensitivity enhancements conferred by dynamic nuclear polarization (DNP), magic angle spinning (MAS) solid state NMR spectroscopy experiments can attain the necessary sensitivity to detect very low concentrations of proteins. This potentially enables structural investigations of proteins at their endogenous levels in their biological contexts where their native stoichiometries with potential interactors is maintained. Yet, even with DNP, experiments are still...
nmrlearner
Journal club
0
03-24-2024 06:32 AM
[NMR paper] Visualizing Proteins in Human Cells at Near-Physiological Concentrations with*Sensitive 19F NMR Chemical Tags
Visualizing Proteins in Human Cells at Near-Physiological Concentrations with*Sensitive 19F NMR Chemical Tags
In-cell NMR spectroscopy is an effective tool for observing proteins at atomic resolution in their native cellular environment. However, its utility is limited by its low sensitivity and the extensive line broadening caused by nonspecific interactions in the cells, which is even more pronounced in human cells due to the difficulty of overexpressing or delivering high concentrations of isotopically labeled proteins. Here, we present a high-sensitivity tag (wPSP-6F) containing two...
[NMR paper] Toward protein NMR at physiological concentrations by hyperpolarized water-Finding and mapping uncharted conformational spaces
Toward protein NMR at physiological concentrations by hyperpolarized water-Finding and mapping uncharted conformational spaces
Nuclear magnetic resonance (NMR) spectroscopy is a key method for determining the structural dynamics of proteins in their native solution state. However, the low sensitivity of NMR typically necessitates nonphysiologically high sample concentrations, which often limit the relevance of the recorded data. We show how to use hyperpolarized water by dissolution dynamic nuclear polarization (DDNP) to acquire protein spectra at concentrations of 1 ?M within seconds and...
Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2) - Technology Networks
Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2) - Technology Networks
Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2) Technology NetworksMalaria is a major threat to the public health worldwide as it is effecting populations in tropical and subtropical areas globally. Among those populations are ...
Read here
nmrlearner
Online News
0
01-07-2019 05:49 AM
X-ray Laser Opens New View on Alzheimer Proteins | Technology ... - Technology Networks
X-ray Laser Opens New View on Alzheimer Proteins | Technology ... - Technology Networks
http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcTAIckjsg4w-e6jhX69ThFbA0fGgX7B6hgTB4gIH1XEJ062Skan1CEy65SL_Sna-BLt-X1HS9k
Technology Networks
<img alt="" height="1" width="1">
X-ray Laser Opens New View on Alzheimer Proteins | Technology ...
Technology Networks
Technology Networks is an internationally recognised publisher that provides access to the latest scientific news, products, research, videos and posters.
X-Ray Laser Opens New View On Alzheimer ProteinsPhotonics Online
NMR Upgrade Enables Protein Characterization at Physiological Concentrations - Technology Networks
Linear Mode
