[NMR paper] 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
Chem Commun (Camb). 2016 Jul 29;
Authors: Pan BB, Yang F, Ye Y, Wu Q, Li C, Huber T, Su XC
Abstract
Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of...